Restriction map for
pFN21AE0813
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssKI BssSI BstAPI BstBI
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MroI MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 219 GTATAC GT^[AC][GT]AC
AlwI 669 GATCC GGATC(4/5)
AseI 286 ATTAAT AT^TAAT
AsnI 286 ATTAAT AT^TAAT
BalI 197 TGGCCA TGG^CCA
BbsI 427 GTCTTC GAAGAC(2/6)
BbvCI 517 GCTGAGG CCTCAGC(-5/-2)
BfaI 70 CTAG C^TAG
BpuAI 427 GTCTTC GAAGAC(2/6)
BsaAI 193 TACGTG [CT]AC^GT[GA]
BseRI 371 CTCCTC GAGGAG(10/8)
BsmAI 550 GTCTC GTCTC(1/5)
Bst1107I 219 GTATAC GTA^TAC
BstEII 567 GGTGACC G^GT[ACGT]ACC
BstPI 567 GGTGACC G^GT[ACGT]ACC
BstZ17I 219 GTATAC GTA^TAC
DpnII 669 GATC ^GATC
DsaI 563 CCACGG C^C[GA][CT]GG
EaeI 197 TGGCCA [CT]^GGCC[GA]
EarI 374 CTCTTC CTCTTC(1/4)
EcoO65I 567 GGTGACC G^GT[ACGT]ACC
EcoRV 34 GATATC GAT^ATC
Ksp632I 374 CTCTTC CTCTTC(1/4)
MaeI 70 CTAG C^TAG
MaeII 194 ACGT A^CGT
MboI 669 GATC ^GATC
MluNI 197 TGGCCA TGG^CCA
MscI 197 TGGCCA TGG^CCA
MseI 287 TTAA T^TAA
MspA1I 515 CAGCTG C[AC]G^C[GT]G
NdeII 669 GATC ^GATC
PflMI 189 CCATTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 286 ATTAAT AT^TAAT
PvuII 515 CAGCTG CAG^CTG
Sau3AI 669 GATC ^GATC
SfcI 121 CTACAG C^T[GA][CT]AG
SspI 340 AATATT AAT^ATT
Tru9I 287 TTAA T^TAA
Tsp45I 568 GTGAC ^GT[GC]AC
TspRI 662 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 189 CCATTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AluI 204 AGCT AG^CT
516 AGCT
AvaII 258 GGTCC G^G[AT]CC
334 GGTCC
BanII 280 GGGCTC G[GA]GC[CT]^C
436 GGGCTC
BmyI 280 GGGCTC G[AGT]GC[ACT]^C
436 GGGCTC
BsiYI 189 CCATTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTAACCACGG
BslI 189 CCATTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTAACCACGG
Bsp1286I 280 GGGCTC G[AGT]GC[ACT]^C
436 GGGCTC
BsrI 663 ACTGG ACTGG(1/-1)
98 CCAGT
BstF5I 498 CATCC GGATG(2/0)
711 GGATG
FokI 498 CATCC GGATG(9/13)
711 GGATG
HaeIII 198 GGCC GG^CC
234 GGCC
HinfI 348 GAATC G^A[ACGT]TC
506 GAATC
HphI 567 GGTGA GGTGA(8/7)
666 GGTGA
MaeIII 148 GTAAC ^GT[ACGT]AC
568 GTGAC
TfiI 348 GAATC G^A[AT]TC
506 GAATC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AfaI BbvI BsaJI BsrDI Cfr13I
DdeI EcoT14I Fnu4HI ItaI MboII
MnlI MslI NlaIII RsaI Sau96I
SfaNI StyI TseI Tsp509I