Restriction map for
pFN21AE0810
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 441 GGTACC G^GTACC
AflIII 293 ACATGT A^C[GA][CT]GT
Asp718I 441 GGTACC G^GTACC
AspHI 490 GAGCAC G[AT]GC[AT]^C
AvaII 180 GGTCC G^G[AT]CC
BalI 6 TGGCCA TGG^CCA
BanI 441 GGTACC G^G[CT][GA]CC
BanII 665 GGGCTC G[GA]GC[CT]^C
BlpI 667 GCTCAGC GC^T[ACGT]AGC
BpmI 18 CTCCAG CTGGAG(16/14)
Bpu1102I 667 GCTCAGC GC^T[ACGT]AGC
BseRI 487 GAGGAG GAGGAG(10/8)
BsiHKAI 490 GAGCAC G[AT]GC[AT]^C
BsmAI 454 GTCTC GTCTC(1/5)
BsmI 759 GCATTC GAATGC(1/-1)
BspLU11I 293 ACATGT A^CATGT
BstEII 330 GGTCACC G^GT[ACGT]ACC
BstPI 330 GGTCACC G^GT[ACGT]ACC
BstYI 734 GGATCT [GA]^GATC[CT]
CelII 667 GCTCAGC GC^T[ACGT]AGC
Cfr13I 180 GGTCC G^G[ACGT]CC
DdeI 668 CTCAG C^T[ACGT]AG
DraI 499 TTTAAA TTT^AAA
DsaI 155 CCATGG C^C[GA][CT]GG
EaeI 6 TGGCCA [CT]^GGCC[GA]
EcoO65I 330 GGTCACC G^GT[ACGT]ACC
EcoT14I 155 CCATGG C^C[AT][AT]GG
FauI 89 GCGGG CCCGC(4/6)
HaeIII 7 GGCC GG^CC
KpnI 441 GGTACC GGTAC^C
MaeII 304 ACGT A^CGT
MflI 734 GGATCT [GA]^GATC[CT]
MluNI 6 TGGCCA TGG^CCA
MscI 6 TGGCCA TGG^CCA
MspA1I 670 CAGCTG C[AC]G^C[GT]G
NcoI 155 CCATGG C^CATGG
NdeI 527 CATATG CA^TATG
NlaIV 441 GGTACC GG[ACGT]^[ACGT]CC
PvuII 670 CAGCTG CAG^CTG
Sau96I 180 GGTCC G^G[ACGT]CC
SmlI 807 CTTGAG C^T[CT][GA]AG
SphI 390 GCATGC GCATG^C
StyI 155 CCATGG C^C[AT][AT]GG
XhoII 734 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 89 GCGG CCGC(-3/-1)
160 GCGG
AfaI 297 GTAC GT^AC
442 GTAC
BcnI 140 CCCGG CC^[GC]GG
458 CCCGG
BmyI 490 GAGCAC G[AGT]GC[ACT]^C
665 GGGCTC
BsaJI 155 CCATGG C^C[ACGT][ACGT]GG
446 CCCTGG
BsgI 283 CTGCAC GTGCAG(16/14)
622 GTGCAG
Bsp1286I 490 GAGCAC G[AGT]GC[ACT]^C
665 GGGCTC
BsrI 404 CCAGT ACTGG(1/-1)
723 CCAGT
EcoRI 24 GAATTC G^AATTC
373 GAATTC
HapII 141 CCGG C^CGG
459 CCGG
HgaI 420 GACGC GACGC(5/10)
756 GACGC
HinfI 467 GAATC G^A[ACGT]TC
582 GATTC
HpaII 141 CCGG C^CGG
459 CCGG
MspI 141 CCGG C^CGG
459 CCGG
NciI 140 CCCGG CC^[GC]GG
458 CCCGG
RsaI 297 GTAC GT^AC
442 GTAC
SfcI 41 CTATAG C^T[GA][CT]AG
378 CTACAG
TfiI 467 GAATC G^A[AT]TC
582 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BbvI BpuAI BssKI BstF5I BstNI
Cac8I DpnII EcoRII Fnu4HI FokI
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI Sau3AI ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI