(Circular) (Six-base) MAPSORT of: pFN21AE0810.seq Check: 6774 from: 1 to: 5620 pFN21AE0810 5620 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2446 2941 2446 Size: 495 5125 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3683 5067 5335 1632 Size: 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 2298 4519 5496 1203 Size: 1095 2221 977 1327 Fragments arranged by size: 2221 1327 1095 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2534 1166 Size: 1368 4252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2787 2848 1184 Size: 1603 61 3956 Fragments arranged by size: 3956 1603 61 AlwNI CAG_nnn'CTG Cuts at: 3363 4935 3363 Size: 1572 4048 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2029 2270 2378 2507 2929 3155 3307 5416 Size: 536 241 108 129 422 226 152 2109 Cuts at: 5416 1493 Size: 1697 Fragments arranged by size: 2109 1697 536 422 241 226 152 129 108 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2446 2941 4396 618 Size: 325 217 627 659 495 1455 1842 Fragments arranged by size: 1842 1455 659 627 495 325 217 BanII G_rGCy'C Cuts at: 1391 2674 2939 1391 Size: 1283 265 4072 Fragments arranged by size: 4072 1283 265 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2590 2599 928 Size: 971 347 344 9 3949 Fragments arranged by size: 3949 971 347 344 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3811 3845 1903 Size: 34 50 34 1790 34 3678 Fragments arranged by size: 3678 1790 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 2673 3037 2673 Size: 364 5256 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 4280 1239 Size: 769 2272 2579 Fragments arranged by size: 2579 2272 769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2833 3745 4610 4908 2833 Size: 912 865 298 3545 Fragments arranged by size: 3545 912 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 1178 Size: 30 165 30 603 30 4762 Fragments arranged by size: 4762 603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 1186 Size: 1321 4299 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 2648 1163 Size: 573 537 375 4135 Fragments arranged by size: 4135 573 537 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 3681 3766 4837 2499 Size: 1182 85 1071 3282 Fragments arranged by size: 3282 1182 1071 85 BsmI GAATG_Cn' Cuts at: 2764 3131 3224 2764 Size: 367 93 5160 Fragments arranged by size: 5160 367 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2674 2939 3681 3766 4837 1391 Size: 399 709 175 265 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 709 399 265 175 85 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 2335 1184 Size: 1151 4469 BstYI r'GATC_y Cuts at: 1972 2739 2950 3317 3702 3719 5171 5257 5269 Size: 767 211 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 767 385 367 346 211 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 513 Size: 552 1095 3973 Fragments arranged by size: 3973 1095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2506 2924 2506 Size: 418 5202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 3067 3956 Size: 54 1425 50 275 18 181 1056 889 Cuts at: 3956 5585 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1056 889 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3550 1353 Size: 2197 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 3683 4280 5067 5335 1239 Size: 393 376 1675 597 787 268 1524 Fragments arranged by size: 1675 1524 787 597 393 376 268 EcoRI G'AATT_C Cuts at: 2029 2378 2929 3307 2029 Size: 349 551 378 4342 Fragments arranged by size: 4342 551 378 349 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2334 2366 4261 4293 4335 Size: 32 135 32 762 32 1895 32 42 Cuts at: 4335 4367 1373 Size: 32 2626 Fragments arranged by size: 2626 1895 762 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2450 2945 2450 Size: 495 5125 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2013 10 Size: 54 1425 343 181 3617 Fragments arranged by size: 3617 1425 343 181 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2534 2615 2769 3578 3937 4096 Size: 648 1131 237 81 154 809 359 159 Cuts at: 4096 518 Size: 2042 Fragments arranged by size: 2042 1131 809 648 359 237 159 154 81 MspA1I CmG'CkG Cuts at: 1606 2677 3036 3713 4861 5106 1606 Size: 1071 359 677 1148 245 2120 Fragments arranged by size: 2120 1148 1071 677 359 245 NcoI C'CATG_G Cuts at: 513 1065 2160 513 Size: 552 1095 3973 Fragments arranged by size: 3973 1095 552 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 3474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2302 2399 2422 2978 4523 1132 Size: 97 1073 97 23 556 1545 2229 Fragments arranged by size: 2229 1545 1073 556 97 97 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 2298 4519 2298 Size: 2221 3399 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PfoI T'CCnGG_A Cuts at: 2024 2024 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 2799 3190 2799 Size: 391 5229 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2294 2326 2294 Size: 32 5588 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 PvuII CAG'CTG Cuts at: 2677 2677 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2046 2383 2968 4103 4784 4975 826 Size: 219 1001 337 585 1135 681 191 1471 Fragments arranged by size: 1471 1135 1001 681 585 337 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2812 3724 4625 4887 820 Size: 197 926 869 912 901 262 1553 Fragments arranged by size: 1553 926 912 901 869 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2399 2978 1132 Size: 1267 579 3774 Fragments arranged by size: 3774 1267 579 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2160 3059 513 Size: 552 1095 899 3074 Fragments arranged by size: 3074 1095 899 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 3647 3664 3817 4002 4447 325 Size: 1255 513 1554 17 153 185 445 1498 Fragments arranged by size: 1554 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 5580 5612 2006 Size: 32 3542 32 2014 Fragments arranged by size: 3542 2014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI