Restriction map for
pFN21AE0810
(Linear) (Six-base) MAPSORT of: KIEE0810.seq Check: 4826 from: 1 to: 915
KIEE0810.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
Acc65I G'GTAC_C
Cuts at: 0 441 915
Size: 441 474
AflIII A'CryG_T
Cuts at: 0 293 915
Size: 293 622
AleI CACnn'nnGTG
Cuts at: 0 529 915
Size: 529 386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 782 843 915
Size: 782 61 72
Fragments arranged by size:
782 72 61
ApoI r'AATT_y
Cuts at: 0 24 265 373 502 915
Size: 24 241 108 129 413
Fragments arranged by size:
413 241 129 108 24
BanI G'GyrC_C
Cuts at: 0 441 915
Size: 441 474
BanII G_rGCy'C
Cuts at: 0 669 915
Size: 669 246
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 585 594 915
Size: 241 344 9 321
Fragments arranged by size:
344 321 241 9
BlpI GC'TnA_GC
Cuts at: 0 668 915
Size: 668 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 915
Size: 3 912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 915
Size: 828 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 915
Size: 1 30 884
Fragments arranged by size:
884 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 915
Size: 502 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 643 915
Size: 268 375 272
Fragments arranged by size:
375 272 268
BsiHKAI G_wGCw'C
Cuts at: 0 494 915
Size: 494 421
BsmI GAATG_Cn'
Cuts at: 0 759 915
Size: 759 156
Bsp1286I G_dGCh'C
Cuts at: 0 494 669 915
Size: 494 175 246
Fragments arranged by size:
494 246 175
BstEII G'GTnAC_C
Cuts at: 0 330 915
Size: 330 585
BstYI r'GATC_y
Cuts at: 0 734 915
Size: 734 181
BtgI C'CryG_G
Cuts at: 0 155 915
Size: 155 760
DraI TTT'AAA
Cuts at: 0 501 915
Size: 501 414
EaeI y'GGCC_r
Cuts at: 0 6 915
Size: 6 909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 915
Size: 3 912
EcoRI G'AATT_C
Cuts at: 0 24 373 915
Size: 24 349 542
Fragments arranged by size:
542 349 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 915
Size: 329 32 554
Fragments arranged by size:
554 329 32
KpnI G_GTAC'C
Cuts at: 0 445 915
Size: 445 470
MscI TGG'CCA
Cuts at: 0 8 915
Size: 8 907
MslI CAynn'nnrTG
Cuts at: 0 292 529 610 764 915
Size: 292 237 81 154 151
Fragments arranged by size:
292 237 154 151 81
MspA1I CmG'CkG
Cuts at: 0 672 915
Size: 672 243
NcoI C'CATG_G
Cuts at: 0 155 915
Size: 155 760
NdeI CA'TA_TG
Cuts at: 0 528 915
Size: 528 387
NspI r_CATG'y
Cuts at: 0 297 394 417 915
Size: 297 97 23 498
Fragments arranged by size:
498 297 97 23
PciI A'CATG_T
Cuts at: 0 293 915
Size: 293 622
PfoI T'CCnGG_A
Cuts at: 0 19 915
Size: 19 896
PsiI TTA'TAA
Cuts at: 0 794 915
Size: 794 121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 289 321 915
Size: 289 32 594
Fragments arranged by size:
594 289 32
PvuII CAG'CTG
Cuts at: 0 672 915
Size: 672 243
SfcI C'TryA_G
Cuts at: 0 41 378 915
Size: 41 337 537
Fragments arranged by size:
537 337 41
SmlI C'TyrA_G
Cuts at: 0 807 915
Size: 807 108
SphI G_CATG'C
Cuts at: 0 394 915
Size: 394 521
StyI C'CwwG_G
Cuts at: 0 155 915
Size: 155 760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 915
Size: 88 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 915
Size: 1 32 882
Fragments arranged by size:
882 32 1
Enzymes that do cut:
Acc65I AflIII AleI AlfI ApoI BanI BanII BbsI
BlpI BpmI BpuEI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BstEII BstYI BtgI DraI EaeI Eco57MI EcoRI
Hin4I KpnI MscI MslI MspA1I NcoI NdeI NspI
PciI PfoI PsiI PsrI PvuII SfcI SmlI SphI
StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI