Restriction map for
pFN21AE0809
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeII MamI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 117 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 898 GTGCTC G[AT]GC[AT]^C
AvaII 713 GGACC G^G[AT]CC
AvrII 882 CCTAGG C^CTAGG
BalI 167 TGGCCA TGG^CCA
BclI 293 TGATCA T^GATCA
BlnI 882 CCTAGG C^CTAGG
BmyI 898 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 898 GTGCTC G[AT]GC[AT]^C
BsmAI 119 GTCTC GTCTC(1/5)
Bsp1286I 898 GTGCTC G[AGT]GC[ACT]^C
BspDI 635 ATCGAT AT^CGAT
BspHI 347 TCATGA T^CATGA
BspMI 741 GCAGGT ACCTGC(4/8)
BsrI 730 ACTGG ACTGG(1/-1)
BstBI 596 TTCGAA TT^CGAA
BstF5I 242 GGATG GGATG(2/0)
ClaI 635 ATCGAT AT^CGAT
DdeI 990 CTCAG C^T[ACGT]AG
DraII 367 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 490 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 299 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 367 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 293 TGATCA T^GATCA
FokI 242 GGATG GGATG(9/13)
MluNI 167 TGGCCA TGG^CCA
MscI 167 TGGCCA TGG^CCA
MspA1I 266 CAGCTG C[AC]G^C[GT]G
NcoI 620 CCATGG C^CATGG
NspV 596 TTCGAA TT^CGAA
PleI 648 GACTC GAGTC(4/5)
PstI 862 CTGCAG CTGCA^G
PvuII 266 CAGCTG CAG^CTG
RcaI 347 TCATGA T^CATGA
SfcI 862 CTGCAG C^T[GA][CT]AG
SfuI 596 TTCGAA TT^CGAA
SmlI 565 CTCAAG C^T[CT][GA]AG
SpeI 69 ACTAGT A^CTAGT
Tsp509I 934 AATT ^AATT
XcmI 99 CCACAAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 308 GGTACC G^GTACC
785 GGTACC
Asp718I 308 GGTACC G^GTACC
785 GGTACC
BanI 308 GGTACC G^G[CT][GA]CC
785 GGTACC
BbsI 222 GAAGAC GAAGAC(2/6)
544 GTCTTC
BcnI 365 CCGGG CC^[GC]GG
380 CCGGG
BpuAI 222 GAAGAC GAAGAC(2/6)
544 GTCTTC
BseRI 535 CTCCTC GAGGAG(10/8)
913 CTCCTC
BsrDI 54 CATTGC GCAATG(2/0)
375 CATTGC
DsaI 620 CCATGG C^C[GA][CT]GG
643 CCACGG
EcoT14I 620 CCATGG C^C[AT][AT]GG
882 CCTAGG
HapII 365 CCGG C^CGG
380 CCGG
HpaII 365 CCGG C^CGG
380 CCGG
KpnI 308 GGTACC GGTAC^C
785 GGTACC
MspI 365 CCGG C^CGG
380 CCGG
NciI 365 CCGGG CC^[GC]GG
380 CCGGG
NspI 560 ACATGC [GA]CATG^[CT]
609 ACATGC
StyI 620 CCATGG C^C[AT][AT]GG
882 CCTAGG
TaqI 597 TCGA T^CGA
636 TCGA
TspRI 460 CAGTG CA[GC]TG[ACGT][ACGT]^
729 CACTG
TthHB8I 597 TCGA T^CGA
636 TCGA
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BpmI BsaJI BsgI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DpnII EaeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TfiI TseI
Tsp45I