Restriction map for
pFN21AE0809
(Linear) (Six-base) MAPSORT of: KIEE0809.seq Check: 6365 from: 1 to: 1005
KIEE0809.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 732 1005
Size: 732 273
Acc65I G'GTAC_C
Cuts at: 0 308 785 1005
Size: 308 477 220
Fragments arranged by size:
477 308 220
AleI CACnn'nnGTG
Cuts at: 0 70 1005
Size: 70 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 1005
Size: 832 173
AlwNI CAG_nnn'CTG
Cuts at: 0 122 1005
Size: 122 883
AvrII C'CTAG_G
Cuts at: 0 882 1005
Size: 882 123
BanI G'GyrC_C
Cuts at: 0 308 785 1005
Size: 308 477 220
Fragments arranged by size:
477 308 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 537 1005
Size: 229 308 468
Fragments arranged by size:
468 308 229
BclI T'GATC_A
Cuts at: 0 293 1005
Size: 293 712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 719 1005
Size: 719 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 647 969 1005
Size: 183 464 322 36
Fragments arranged by size:
464 322 183 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1005
Size: 550 455
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 1005
Size: 181 30 794
Fragments arranged by size:
794 181 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 904 1005
Size: 526 378 101
Fragments arranged by size:
526 378 101
BseYI C'CCAG_C
Cuts at: 0 525 652 1005
Size: 525 127 353
Fragments arranged by size:
525 353 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 483 760 1005
Size: 252 231 277 245
Fragments arranged by size:
277 252 245 231
BsiHKAI G_wGCw'C
Cuts at: 0 902 1005
Size: 902 103
Bsp1286I G_dGCh'C
Cuts at: 0 902 1005
Size: 902 103
BspHI T'CATG_A
Cuts at: 0 347 1005
Size: 347 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 732 1005
Size: 732 273
BsrDI GCAATG_nn'
Cuts at: 0 53 374 1005
Size: 53 321 631
Fragments arranged by size:
631 321 53
BstBI TT'CG_AA
Cuts at: 0 597 1005
Size: 597 408
BtgI C'CryG_G
Cuts at: 0 620 643 1005
Size: 620 23 362
Fragments arranged by size:
620 362 23
BtsI GCAGTG_nn'
Cuts at: 0 466 1005
Size: 466 539
ClaI AT'CG_AT
Cuts at: 0 636 1005
Size: 636 369
DraIII CAC_nnn'GTG
Cuts at: 0 495 1005
Size: 495 510
EaeI y'GGCC_r
Cuts at: 0 167 362 926 1005
Size: 167 195 564 79
Fragments arranged by size:
564 195 167 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 647 969 1005
Size: 183 464 322 36
Fragments arranged by size:
464 322 183 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 303 1005
Size: 303 702
EcoO109I rG'GnC_Cy
Cuts at: 0 368 1005
Size: 368 637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 1005
Size: 345 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 851 883 1005
Size: 181 32 638 32 122
Fragments arranged by size:
638 181 122 32 32
KpnI G_GTAC'C
Cuts at: 0 312 789 1005
Size: 312 477 216
Fragments arranged by size:
477 312 216
MscI TGG'CCA
Cuts at: 0 169 1005
Size: 169 836
MslI CAynn'nnrTG
Cuts at: 0 70 104 286 1005
Size: 70 34 182 719
Fragments arranged by size:
719 182 70 34
MspA1I CmG'CkG
Cuts at: 0 268 1005
Size: 268 737
NcoI C'CATG_G
Cuts at: 0 620 1005
Size: 620 385
NspI r_CATG'y
Cuts at: 0 564 613 1005
Size: 564 49 392
Fragments arranged by size:
564 392 49
PasI CC'CwG_GG
Cuts at: 0 953 1005
Size: 953 52
PfoI T'CCnGG_A
Cuts at: 0 709 1005
Size: 709 296
PstI C_TGCA'G
Cuts at: 0 866 1005
Size: 866 139
PvuII CAG'CTG
Cuts at: 0 268 1005
Size: 268 737
SfcI C'TryA_G
Cuts at: 0 862 1005
Size: 862 143
SmlI C'TyrA_G
Cuts at: 0 565 1005
Size: 565 440
SpeI A'CTAG_T
Cuts at: 0 69 1005
Size: 69 936
StyI C'CwwG_G
Cuts at: 0 620 882 1005
Size: 620 262 123
Fragments arranged by size:
620 262 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 410 442 1005
Size: 410 32 563
Fragments arranged by size:
563 410 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 1005
Size: 106 899
Enzymes that do cut:
AarI Acc65I AleI AlfI AlwNI AvrII BanI BbsI
BclI BplI BpmI BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BstBI BtgI BtsI
ClaI DraIII EaeI Eco57MI EcoNI EcoO109I FalI Hin4I
KpnI MscI MslI MspA1I NcoI NspI PasI PfoI
PstI PvuII SfcI SmlI SpeI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI XmnI ZraI