Restriction map for
pFN21AE0793
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 909 CGCG CG^CG
AflIII 303 ACACGT A^C[GA][CT]GT
AlwNI 446 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 904 GGGCCC GGGCC^C
BbsI 543 GAAGAC GAAGAC(2/6)
BfaI 440 CTAG C^TAG
BglI 291 GCCCAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 820 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 820 GCTCAGC GC^T[ACGT]AGC
BpuAI 543 GAAGAC GAAGAC(2/6)
BsaWI 323 TCCGGT [AT]^CCGG[AT]
BsmAI 307 GTCTC GTCTC(1/5)
BsmBI 306 CGTCTC CGTCTC(1/5)
Bsp1407I 140 TGTACA T^GTACA
BsrFI 646 ACCGGC [GA]^CCGG[CT]
BsrGI 140 TGTACA T^GTACA
BstNI 172 CCAGG CC^[AT]GG
BstUI 909 CGCG CG^CG
CelII 820 GCTCAGC GC^T[ACGT]AGC
Cfr10I 646 ACCGGC [GA]^CCGG[CT]
EaeI 102 CGGCCA [CT]^GGCC[GA]
EcoRI 610 GAATTC G^AATTC
EcoRII 172 CCAGG ^CC[AT]GG
EcoT14I 410 CCAAGG C^C[AT][AT]GG
HinfI 866 GAGTC G^A[ACGT]TC
KspI 908 CCGCGG CCGC^GG
MaeI 440 CTAG C^TAG
MseI 395 TTAA T^TAA
MvaI 172 CCAGG CC^[AT]GG
MvnI 909 CGCG CG^CG
PflMI 563 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 866 GAGTC GAGTC(4/5)
PshAI 108 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 830 CTGCAG CTGCA^G
PvuI 482 CGATCG CGAT^CG
PvuII 875 CAGCTG CAG^CTG
SacII 908 CCGCGG CCGC^GG
SmaI 461 CCCGGG CCC^GGG
SspBI 140 TGTACA T^GTACA
StyI 410 CCAAGG C^C[AT][AT]GG
Tru9I 395 TTAA T^TAA
Tsp45I 622 GTGAC ^GT[GC]AC
Van91I 563 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 461 CCCGGG C^CCGGG
XmaI 461 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 598 GTCTAC GT^[AC][GT]AC
868 GTCTAC
AcsI 371 AAATTT [GA]^AATT[CT]
610 GAATTC
ApoI 371 AAATTT [GA]^AATT[CT]
610 GAATTC
BsiEI 482 CGATCG CG[GA][CT]^CG
755 CGGTCG
BsrI 24 CCAGT ACTGG(1/-1)
583 CCAGT
DpnII 387 GATC ^GATC
483 GATC
DraII 205 GGGACCC [GA]G^G[ACGT]CC[CT]
332 GGGACCC
DsaI 518 CCACGG C^C[GA][CT]GG
908 CCGCGG
EcoO109I 205 GGGACCC [GA]G^G[ACGT]CC[CT]
332 GGGACCC
MaeII 305 ACGT A^CGT
380 ACGT
MboI 387 GATC ^GATC
483 GATC
MboII 543 GAAGA GAAGA(8/7)
678 TCTTC
NdeII 387 GATC ^GATC
483 GATC
PpuMI 205 GGGACCC [GA]G^G[AT]CC[CT]
332 GGGACCC
Sau3AI 387 GATC ^GATC
483 GATC
SfaNI 448 GATGC GCATC(5/9)
50 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BssKI BstF5I Cac8I CfoI
Cfr13I DdeI FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MnlI MspA1I MspI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfcI TseI Tsp509I
TspRI