(Circular) (Six-base) MAPSORT of: pFN21AE0793.seq Check: 8288 from: 1 to: 5641 pFN21AE0793 5641 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 2874 2984 1937 Size: 667 270 110 4594 Fragments arranged by size: 4594 667 270 110 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2308 4540 5517 1203 Size: 1105 2232 977 1327 Fragments arranged by size: 2232 1327 1105 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2456 3384 4956 2456 Size: 928 1572 3141 Fragments arranged by size: 3141 1572 928 ApaI G_GGCC'C Cuts at: 2913 2913 Size: 5641 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2376 2615 2950 3176 3328 5437 1493 Size: 883 239 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 335 239 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2466 2529 2966 1870 Size: 73 225 298 63 437 4545 Fragments arranged by size: 4545 437 298 225 73 63 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2414 2467 2913 2960 1391 Size: 1023 53 446 47 4072 Fragments arranged by size: 4072 1023 446 53 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2555 928 Size: 971 656 4014 Fragments arranged by size: 4014 971 656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2302 4252 136 Size: 107 122 71 631 605 630 1950 1525 Fragments arranged by size: 1950 1525 631 630 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2826 3058 2826 Size: 232 5409 Bme1580I G_kGCm'C Cuts at: 1790 2913 3702 4858 1790 Size: 1123 789 1156 2573 Fragments arranged by size: 2573 1156 1123 789 BmgBI CAC'GTC Cuts at: 1318 2311 2386 1318 Size: 993 75 4573 Fragments arranged by size: 4573 993 75 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 3372 4330 Size: 416 53 83 399 24 606 1349 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1349 958 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2328 4074 4472 4746 4893 5304 2328 Size: 1746 398 274 147 411 2665 Fragments arranged by size: 2665 1746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2748 2778 1178 Size: 30 165 30 1345 30 4041 Fragments arranged by size: 4041 1345 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5641 BseYI C'CCAG_C Cuts at: 2010 4844 5340 2010 Size: 2834 496 2311 Fragments arranged by size: 2834 2311 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2490 2763 3091 3852 4880 1540 Size: 275 186 489 273 328 761 1028 2301 Fragments arranged by size: 2301 1028 761 489 328 275 273 186 BsiHKAI G_wGCw'C Cuts at: 3702 3787 4858 3702 Size: 85 1071 4485 Fragments arranged by size: 4485 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 BsmBI CGTCTCn'nnnn_ Cuts at: 2317 2317 Size: 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2414 2467 2913 2960 3702 3787 4858 Size: 399 624 53 446 47 742 85 1071 Cuts at: 4858 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 624 446 399 85 53 47 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2996 844 Size: 2152 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2263 2407 2759 3528 1394 Size: 429 440 144 352 769 3507 Fragments arranged by size: 3507 769 440 429 352 144 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 4285 4472 1700 Size: 110 841 1634 187 2869 Fragments arranged by size: 2869 1634 841 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 3592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636 Size: 999 367 385 17 1452 86 12 346 Cuts at: 5636 1972 Size: 1977 Fragments arranged by size: 1977 1452 999 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 2913 2937 513 Size: 552 939 519 390 24 3217 Fragments arranged by size: 3217 939 552 519 390 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2188 487 Size: 683 56 962 3940 Fragments arranged by size: 3940 962 683 56 BtsI GCAGTG_nn' Cuts at: 2631 3157 3951 3971 2631 Size: 526 794 20 4301 Fragments arranged by size: 4301 794 526 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 3088 3977 Size: 54 1425 50 275 18 277 981 889 Cuts at: 3977 5606 8 Size: 1629 43 Fragments arranged by size: 1629 1425 981 889 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2031 4228 4602 4748 387 Size: 1064 138 442 2197 374 146 1280 Fragments arranged by size: 2197 1280 1064 442 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3704 4301 5088 5356 1239 Size: 393 2072 597 787 268 1524 Fragments arranged by size: 2072 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2211 2338 2211 Size: 127 5514 EcoRI G'AATT_C Cuts at: 2615 2950 3328 2615 Size: 335 378 4928 Fragments arranged by size: 4928 378 335 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 2340 4755 4939 1305 Size: 993 42 2415 184 2007 Fragments arranged by size: 2415 2007 993 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3599 3958 4117 518 Size: 648 2433 359 159 2042 Fragments arranged by size: 2433 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2834 2840 2882 2915 3057 3734 4882 5127 Size: 1228 6 42 33 142 677 1148 245 Cuts at: 5127 1606 Size: 2120 Fragments arranged by size: 2120 1228 1148 677 245 142 42 33 6 NcoI C'CATG_G Cuts at: 513 1065 2004 2937 513 Size: 552 939 933 3217 Fragments arranged by size: 3217 939 933 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2574 1108 Size: 1466 4175 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2211 2338 2211 Size: 127 5514 PshAI GACnn'nnGTC Cuts at: 1613 2117 1613 Size: 504 5137 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2909 2909 Size: 5641 PstI C_TGCA'G Cuts at: 830 2839 2993 830 Size: 2009 154 3478 Fragments arranged by size: 3478 2009 154 PvuI CG_AT'CG Cuts at: 2001 2490 2001 Size: 489 5152 PvuII CAG'CTG Cuts at: 2882 2882 Size: 5641 SacII CC_GC'GG Cuts at: 2916 2916 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SanDI GG'GwC_CC Cuts at: 2211 2338 2211 Size: 127 5514 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2280 2815 2835 2851 2923 2989 4124 Size: 219 1235 535 20 16 72 66 1135 Cuts at: 4124 4805 4996 826 Size: 681 191 1471 Fragments arranged by size: 1471 1235 1135 681 535 219 191 72 66 20 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2468 2968 1872 Size: 596 500 4545 Fragments arranged by size: 4545 596 500 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2415 2937 3080 513 Size: 552 939 411 522 143 3074 Fragments arranged by size: 3074 939 552 522 411 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2475 3668 3685 3838 4023 4468 325 Size: 1255 895 1193 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1193 895 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3887 5599 Size: 274 80 33 51 1443 168 1742 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1742 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2727 2748 2759 2780 5601 5633 2727 Size: 21 11 21 2821 32 2735 Fragments arranged by size: 2821 2735 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2466 2966 1870 Size: 596 500 4545 Fragments arranged by size: 4545 596 500 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI