(Circular) (Six-base) MAPSORT of: pFN21AE0790.seq Check: 1416 from: 1 to: 6091 pFN21AE0790 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2710 2962 4154 5538 5806 1632 Size: 1078 252 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 1078 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2099 2436 2502 2760 2844 2987 3264 3348 Size: 606 337 66 258 84 143 277 84 Cuts at: 3348 3364 3400 3626 3778 5887 1493 Size: 16 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 606 337 277 258 226 152 143 84 84 66 36 16 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3416 1870 Size: 73 1473 4545 Fragments arranged by size: 4545 1473 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3412 4867 618 Size: 325 217 627 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2250 3410 1391 Size: 859 1160 4072 Fragments arranged by size: 4072 1160 859 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2195 2716 3304 928 Size: 971 296 521 588 3715 Fragments arranged by size: 3715 971 588 521 296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4282 4316 1903 Size: 34 50 34 2261 34 3678 Fragments arranged by size: 3678 2261 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BfrBI ATG'CAT Cuts at: 3299 3299 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 4152 5308 1790 Size: 2362 1156 2573 Fragments arranged by size: 2573 2362 1156 BmgBI CAC'GTC Cuts at: 1318 2089 2158 1318 Size: 771 69 5251 Fragments arranged by size: 5251 771 69 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2874 2958 3210 3294 3378 4751 1239 Size: 1635 84 252 84 84 1373 2579 Fragments arranged by size: 2579 1635 1373 252 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 4216 5081 5379 2411 Size: 1805 865 298 3123 Fragments arranged by size: 3123 1805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3274 4754 882 Size: 617 1775 1480 2219 Fragments arranged by size: 2219 1775 1480 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2317 2347 1178 Size: 30 165 30 914 30 4922 Fragments arranged by size: 4922 914 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2341 1186 Size: 940 215 4936 Fragments arranged by size: 4936 940 215 BseYI C'CCAG_C Cuts at: 5294 5790 5294 Size: 496 5595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 3410 4152 4237 5308 1391 Size: 399 460 1160 742 85 1071 2174 Fragments arranged by size: 2174 1160 1071 742 460 399 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2353 4586 4760 65 Size: 1183 1105 2233 174 1396 Fragments arranged by size: 2233 1396 1183 1105 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2064 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3368 3607 4401 4421 3368 Size: 239 794 20 5038 Fragments arranged by size: 5038 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3538 4427 Size: 54 1425 50 275 18 261 1447 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1447 1425 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2064 2574 3133 4021 1353 Size: 671 40 510 559 888 3423 Fragments arranged by size: 3423 888 671 559 510 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3038 4678 5052 5198 387 Size: 1064 138 1449 1640 374 146 1280 Fragments arranged by size: 1640 1449 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2710 2874 2958 2962 3210 3294 3378 Size: 393 1078 164 84 4 248 84 84 Cuts at: 3378 4154 4751 5538 5806 1239 Size: 776 597 787 268 1524 Fragments arranged by size: 1524 1078 787 776 597 393 268 248 164 84 84 84 4 EcoRI G'AATT_C Cuts at: 2099 2760 2844 3264 3364 3400 3778 2099 Size: 661 84 420 100 36 378 4412 Fragments arranged by size: 4412 661 420 378 100 84 36 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 4732 4764 4806 Size: 32 135 32 533 32 2595 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 2595 533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 2889 748 Size: 2141 3950 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 5205 5389 1305 Size: 840 3060 184 2007 Fragments arranged by size: 3060 2007 840 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 10 Size: 54 1425 343 261 4008 Fragments arranged by size: 4008 1425 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 4049 4408 4567 518 Size: 648 921 1962 359 159 2042 Fragments arranged by size: 2042 1962 921 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 3507 4184 5332 5577 1606 Size: 810 1091 677 1148 245 2120 Fragments arranged by size: 2120 1148 1091 810 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NsiI A_TGCA'T Cuts at: 3301 3301 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3303 3449 4994 1132 Size: 97 2074 146 1545 2229 Fragments arranged by size: 2229 2074 1545 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2416 2416 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 2064 3133 2064 Size: 1069 5022 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 2181 2181 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2173 3439 4574 5255 5446 826 Size: 219 1128 1266 1135 681 191 1471 Fragments arranged by size: 1471 1266 1135 1128 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2390 4195 5096 5358 820 Size: 197 926 447 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 447 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 3530 513 Size: 552 2465 3074 Fragments arranged by size: 3074 2465 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4118 4135 4288 4473 4918 325 Size: 1255 2538 17 153 185 445 1498 Fragments arranged by size: 2538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2705 3104 4337 Size: 274 80 33 51 1443 728 399 1233 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1233 728 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2317 2349 6051 6083 2317 Size: 32 3702 32 2325 Fragments arranged by size: 3702 2325 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3224 1316 Size: 1908 4183 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI