Restriction map for
pFN21AE0790
(Linear) (Six-base) MAPSORT of: KIEE0790.seq Check: 4302 from: 1 to: 1386
KIEE0790.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 957 1386
Size: 705 252 429
Fragments arranged by size:
705 429 252
ApoI r'AATT_y
Cuts at: 0 94 431 497 755 839 982 1259 1343
Size: 94 337 66 258 84 143 277 84
Cuts at: 1343 1359 1386
Size: 16 27
Fragments arranged by size:
337 277 258 143 94 84 84 66
27 16
BanII G_rGCy'C
Cuts at: 0 245 1386
Size: 245 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 190 711 1299 1386
Size: 190 521 588 87
Fragments arranged by size:
588 521 190 87
BfrBI ATG'CAT
Cuts at: 0 1294 1386
Size: 1294 92
BmgBI CAC'GTC
Cuts at: 0 84 153 1386
Size: 84 69 1233
Fragments arranged by size:
1233 84 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 953 1205 1289 1373 1386
Size: 869 84 252 84 84 13
Fragments arranged by size:
869 252 84 84 84 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1386
Size: 406 980
BsaI GGTCTCn'nnnn_
Cuts at: 0 1269 1386
Size: 1269 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 312 342 1386
Size: 312 30 1044
Fragments arranged by size:
1044 312 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 336 1386
Size: 121 215 1050
Fragments arranged by size:
1050 215 121
Bsp1286I G_dGCh'C
Cuts at: 0 245 1386
Size: 245 1141
BsrDI GCAATG_nn'
Cuts at: 0 348 1386
Size: 348 1038
Bsu36I CC'TnA_GG
Cuts at: 0 59 1386
Size: 59 1327
BtsI GCAGTG_nn'
Cuts at: 0 1363 1386
Size: 1363 23
EaeI y'GGCC_r
Cuts at: 0 86 1386
Size: 86 1300
EarI CTCTTCn'nnn_
Cuts at: 0 19 59 569 1128 1386
Size: 19 40 510 559 258
Fragments arranged by size:
559 510 258 40 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1033 1386
Size: 1033 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 869 953 957 1205 1289 1373 1386
Size: 705 164 84 4 248 84 84 13
Fragments arranged by size:
705 248 164 84 84 84 13 4
EcoRI G'AATT_C
Cuts at: 0 94 755 839 1259 1359 1386
Size: 94 661 84 420 100 27
Fragments arranged by size:
661 420 100 94 84 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1386
Size: 100 32 1254
Fragments arranged by size:
1254 100 32
HindIII A'AGCT_T
Cuts at: 0 884 1386
Size: 884 502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1386
Size: 140 1246
MscI TGG'CCA
Cuts at: 0 88 1386
Size: 88 1298
MslI CAynn'nnrTG
Cuts at: 0 82 1386
Size: 82 1304
MspA1I CmG'CkG
Cuts at: 0 411 1386
Size: 411 975
NsiI A_TGCA'T
Cuts at: 0 1296 1386
Size: 1296 90
NspI r_CATG'y
Cuts at: 0 1298 1386
Size: 1298 88
PvuII CAG'CTG
Cuts at: 0 411 1386
Size: 411 975
SapI GCTCTTCn'nnn_
Cuts at: 0 59 1128 1386
Size: 59 1069 258
Fragments arranged by size:
1069 258 59
SexAI A'CCwGG_T
Cuts at: 0 176 1386
Size: 176 1210
SfcI C'TryA_G
Cuts at: 0 168 1386
Size: 168 1218
SmlI C'TyrA_G
Cuts at: 0 385 1386
Size: 385 1001
TatI w'GTAC_w
Cuts at: 0 700 1099 1386
Size: 700 399 287
Fragments arranged by size:
700 399 287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 312 344 1386
Size: 312 32 1042
Fragments arranged by size:
1042 312 32
Tth111I GACn'n_nGTC
Cuts at: 0 1219 1386
Size: 1219 167
Enzymes that do cut:
AcuI ApoI BanII BbsI BfrBI BmgBI BpmI BpuEI
BsaI BsaXI BseRI Bsp1286I BsrDI Bsu36I BtsI EaeI
EarI EciI Eco57MI EcoRI Hin4I HindIII MmeI MscI
MslI MspA1I NsiI NspI PvuII SapI SexAI SfcI
SmlI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII XbaI XcmI XhoI XmaI XmnI ZraI