Restriction map for
pFN21AE0782
(Circular) (Six-base) MAPSORT of: pFN21AE0782.seq Check: 6854 from: 1 to: 6064
pFN21AE0782 6064 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3140 3322 4127 5511 5779 1632
Size: 1508 182 805 1384 268 1917
Fragments arranged by size:
1917 1508 1384 805 268 182
AfeI AGC'GCT
Cuts at: 2019 3068 2019
Size: 1049 5015
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2094 2301 4963 5940 1203
Size: 891 207 2662 977 1327
Fragments arranged by size:
2662 1327 977 891 207
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 2677 4793 2677
Size: 2116 3948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2310 3101 3192 1184
Size: 1126 791 91 4056
Fragments arranged by size:
4056 1126 791 91
AlwNI CAG_nnn'CTG
Cuts at: 3807 5379 3807
Size: 1572 4492
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3159 3184 3373 3599 3751 5860 1493
Size: 1666 25 189 226 152 2109 1697
Fragments arranged by size:
2109 1697 1666 226 189 152 25
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2602 3259 3389 1870
Size: 73 112 547 657 130 4545
Fragments arranged by size:
4545 657 547 130 112 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2276 2375 3385 4840
Size: 325 217 627 284 205 99 1010 1455
Cuts at: 4840 618
Size: 1842
Fragments arranged by size:
1842 1455 1010 627 325 284 217 205
99
BanII G_rGCy'C
Cuts at: 1391 2064 2187 2481 3383 1391
Size: 673 123 294 902 4072
Fragments arranged by size:
4072 902 673 294 123
BbeI G_GCGC'C
Cuts at: 2075 2379 2075
Size: 304 5760
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5093
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2773 2807 3250 3284 4255
Size: 34 50 34 752 34 443 34 971
Cuts at: 4255 4289 1903
Size: 34 3678
Fragments arranged by size:
3678 971 752 443 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 2559 1548
Size: 1011 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2036 2045
Size: 107 122 71 631 605 337 27 9
Cuts at: 2045 2127 4675 136
Size: 82 2548 1525
Fragments arranged by size:
2548 1525 631 605 337 122 107 82
71 27 9
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 2402 3481 2402
Size: 1079 4985
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2289 2517 4125 5281 1790
Size: 349 150 228 1608 1156 2573
Fragments arranged by size:
2573 1608 1156 349 228 150
BmgBI CAC'GTC
Cuts at: 1318 2095 2440 1318
Size: 777 345 4942
Fragments arranged by size:
4942 777 345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2039
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 3326 4724 1239
Size: 1007 1080 1398 2579
Fragments arranged by size:
2579 1398 1080 1007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 2954 4189 5054 5352 2603
Size: 351 1235 865 298 3315
Fragments arranged by size:
3315 1235 865 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 4727 882
Size: 617 897 2331 2219
Fragments arranged by size:
2331 2219 897 617
BsaAI yAC'GTr
Cuts at: 493 1204 2134 2578 3855 493
Size: 711 930 444 1277 2702
Fragments arranged by size:
2702 1277 930 711 444
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2351 2376 3244
Size: 53 83 186 936 539 279 25 868
Cuts at: 3244 3871 4253 4888 275
Size: 627 382 635 1451
Fragments arranged by size:
1451 936 868 635 627 539 382 279
186 83 53 25
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 3277 3307 1178
Size: 30 165 30 1497 30 347 30 3935
Fragments arranged by size:
3935 1497 347 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3285 3323 1186
Size: 2099 38 3927
Fragments arranged by size:
3927 2099 38
BseYI C'CCAG_C
Cuts at: 2108 2734 2835 2854 5267 5763 2108
Size: 626 101 19 2413 496 2409
Fragments arranged by size:
2413 2409 626 496 101 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 1163
Size: 573 909 4582
Fragments arranged by size:
4582 909 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2748 3514 4275 5303 1540
Size: 275 186 747 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 747 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 4125 4210 5281 2481
Size: 1644 85 1071 3264
Fragments arranged by size:
3264 1644 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2139 2187 2289 2481 2517 3383
Size: 399 274 75 48 102 192 36 866
Cuts at: 3383 4125 4210 5281 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 866 742 399 274 192 102
85 75 48 36
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3419 844
Size: 1916 659 3489
Fragments arranged by size:
3489 1916 659
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 2685 4559 4733 65
Size: 1183 1182 255 1874 174 1396
Fragments arranged by size:
1874 1396 1183 1182 255 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2552 4708 4895 1700
Size: 110 742 2156 187 2869
Fragments arranged by size:
2869 2156 742 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 2084 2307 2331 1925
Size: 159 223 24 5658
Fragments arranged by size:
5658 223 159 24
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2154 794
Size: 1360 4704
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2700 3394 3761 4146 4163 5615 5701 5713
Size: 728 694 367 385 17 1452 86 12
Cuts at: 5713 6059 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 728 694 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3351 3351
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2463 513
Size: 552 1398 4114
Fragments arranged by size:
4114 1398 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3328 487
Size: 683 56 2102 3223
Fragments arranged by size:
3223 2102 683 56
BtsI GCAGTG_nn'
Cuts at: 2829 3160 3580 4374 4394 2829
Size: 331 420 794 20 4499
Fragments arranged by size:
4499 794 420 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3090 3125 529
Size: 35 2526 35 3468
Fragments arranged by size:
3468 2526 35 35
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 2269 2581 2269
Size: 312 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3227 5071 809
Size: 507 1911 1844 1802
Fragments arranged by size:
1911 1844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2037 2319
Size: 54 1425 50 275 18 198 9 282
Cuts at: 2319 2503 2745 2775 3511 4400 6029 8
Size: 184 242 30 736 889 1629 43
Fragments arranged by size:
1629 1425 889 736 282 275 242 198
184 54 50 43 30 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2745 3511 1537
Size: 275 933 766 4090
Fragments arranged by size:
4090 933 766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 3341 3994 1353
Size: 1133 855 653 3423
Fragments arranged by size:
3423 1133 855 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2479 2479
Size: 6064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 3140 3322 3326 4127 4724 5511
Size: 393 614 894 182 4 801 597 787
Cuts at: 5511 5779 1239
Size: 268 1524
Fragments arranged by size:
1524 894 801 787 614 597 393 268
182 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3305 3305
Size: 6064
EcoRI G'AATT_C
Cuts at: 3184 3373 3751 3184
Size: 189 378 5497
Fragments arranged by size:
5497 378 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 3085 2443
Size: 642 5422
FseI GG_CCGG'CC
Cuts at: 2556 2556
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2021 2051 2075 2379 3070 5211 1678
Size: 343 30 24 304 691 2141 2531
Fragments arranged by size:
2531 2141 691 343 304 30 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 3192 3224 3277
Size: 32 135 32 1091 32 497 32 53
Cuts at: 3277 3309 4705 4737 4779 4811 1373
Size: 32 1396 32 42 32 2626
Fragments arranged by size:
2626 1396 1091 497 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 3408 3654 669
Size: 988 281 1300 170 246 3079
Fragments arranged by size:
3079 1300 988 281 246 170
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2071 2375 2071
Size: 304 5760
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 2895 5178 5362 1305
Size: 1356 234 2283 184 2007
Fragments arranged by size:
2283 2007 1356 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2777 10
Size: 54 1425 343 945 3297
Fragments arranged by size:
3297 1425 945 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 3083 4022 4381 4540 518
Size: 648 1126 791 939 359 159 2042
Fragments arranged by size:
2042 1126 939 791 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2250 2256 2365 2401 2497 2526 2839
Size: 512 132 6 109 36 96 29 313
Cuts at: 2839 3480 4157 5305 5550 1606
Size: 641 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 512 313 245 132
109 96 36 29 6
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 6064
NarI GG'CG_CC
Cuts at: 2072 2376 2072
Size: 304 5760
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5512
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 3422 4967 1132
Size: 97 2193 1545 2229
Fragments arranged by size:
2229 2193 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2345 2862 2345
Size: 517 5547
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 2578 2578
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PspXI vC'TCGA_Gb
Cuts at: 3259 3259
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2316 2340 2370 3273 3416 830
Size: 1486 24 30 903 143 3478
Fragments arranged by size:
3478 1486 903 143 30 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2365 2401 2365
Size: 36 6028
SacI G_AGCT'C
Cuts at: 2481 2481
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SapI GCTCTTCn'nnn_
Cuts at: 2486 2486
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2312 2336 2366 2630 2798 3269 3412
Size: 219 1267 24 30 264 168 471 143
Cuts at: 3412 4547 5228 5419 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1267 1135 681 471 264 219 191
168 143 30 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2036 2036
Size: 6064
SfoI GGC'GCC
Cuts at: 2073 2377 2073
Size: 304 5760
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 2057 2604 3391 1872
Size: 185 547 787 4545
Fragments arranged by size:
4545 787 547 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 2969 3259 4168 5069 5331
Size: 197 926 675 351 290 909 901 262
Cuts at: 5331 820
Size: 1553
Fragments arranged by size:
1553 926 909 901 675 351 290 262
197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 3503 513
Size: 552 2438 3074
Fragments arranged by size:
3074 2438 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3302 4091 4108 4261 4446 4891 325
Size: 1255 1722 789 17 153 185 445 1498
Fragments arranged by size:
1722 1498 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2916 2948 3150 3182 6024 6056 2567
Size: 32 317 32 202 32 2842 32 2575
Fragments arranged by size:
2842 2575 317 202 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3289 1316
Size: 1973 4091
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 3259 1943
Size: 1316 4748
XmaI C'CCGG_G
Cuts at: 1870 2055 2602 3389 1870
Size: 185 547 787 4545
Fragments arranged by size:
4545 787 547 185
XmnI GAAnn'nnTTC
Cuts at: 3068 4193 3068
Size: 1125 4939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoO109I EcoRV FspAI
NruI NsiI PacI PpuMI PspOMI PsrI RsrII SacII
SanDI SexAI SrfI SwaI