(Circular) (Six-base) MAPSORT of: pFN21AE0781.seq Check: 4772 from: 1 to: 5755 pFN21AE0781 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2780 2883 3818 5202 5470 1632 Size: 1148 103 935 1384 268 1917 Fragments arranged by size: 1917 1384 1148 935 268 103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2589 4654 5631 1203 Size: 1386 2065 977 1327 Fragments arranged by size: 2065 1386 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 2972 3812 4968 2972 Size: 840 1156 3759 Fragments arranged by size: 3759 1156 840 ApoI r'AATT_y Cuts at: 1493 2105 2327 2689 2723 3064 3290 3442 5551 Size: 612 222 362 34 341 226 152 2109 Cuts at: 5551 1493 Size: 1697 Fragments arranged by size: 2109 1697 612 362 341 226 222 152 34 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 3076 4531 618 Size: 325 217 627 597 692 1455 1842 Fragments arranged by size: 1842 1455 692 627 597 325 217 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2820 928 Size: 971 921 3863 Fragments arranged by size: 3863 971 921 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2512 3647 4857 2512 Size: 1135 1210 3410 Fragments arranged by size: 3410 1210 1135 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BfrBI ATG'CAT Cuts at: 2310 2310 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2532 4366 136 Size: 107 122 71 631 605 860 1834 1525 Fragments arranged by size: 1834 1525 860 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2976 3816 4972 1790 Size: 1186 840 1156 2573 Fragments arranged by size: 2573 1186 1156 840 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3057 3486 4444 Size: 416 53 83 399 24 1640 429 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1640 958 429 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2929 2929 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2604 4415 1239 Size: 1043 322 1811 2579 Fragments arranged by size: 2579 1811 1043 322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2827 2899 3880 4745 5043 2827 Size: 72 981 865 298 3539 Fragments arranged by size: 3539 981 865 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2064 3546 493 Size: 711 860 1482 2702 Fragments arranged by size: 2702 1482 860 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 1186 Size: 1093 4662 BseYI C'CCAG_C Cuts at: 2846 4958 5454 2846 Size: 2112 496 3147 Fragments arranged by size: 3147 2112 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 2813 1163 Size: 573 886 191 4105 Fragments arranged by size: 4105 886 573 191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 2976 3816 3901 4972 2165 Size: 811 840 85 1071 2948 Fragments arranged by size: 2948 1071 840 811 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2976 3074 3816 3901 4972 1391 Size: 399 375 811 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 811 742 399 375 98 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 2538 3644 5374 2538 Size: 1106 1730 2919 Fragments arranged by size: 2919 1730 1106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2835 3110 844 Size: 1991 275 3489 Fragments arranged by size: 3489 1991 275 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 3034 3034 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2857 3085 3452 3837 3854 5306 5392 5404 Size: 885 228 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 885 385 367 346 228 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2535 487 Size: 683 56 1309 3707 Fragments arranged by size: 3707 1309 683 56 BtsI GCAGTG_nn' Cuts at: 2464 2610 3271 4065 4085 2464 Size: 146 661 794 20 4134 Fragments arranged by size: 4134 794 661 146 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 3015 3015 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720 Size: 54 1425 50 275 18 1372 889 1629 Cuts at: 5720 8 Size: 43 Fragments arranged by size: 1629 1425 1372 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 2921 2931 3685 1353 Size: 1326 242 10 754 3423 Fragments arranged by size: 3423 1326 754 242 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2604 2780 2883 3818 4415 5202 Size: 393 650 322 176 103 935 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 935 787 650 597 393 322 268 176 103 EcoRI G'AATT_C Cuts at: 2105 2689 2723 3064 3442 2105 Size: 584 34 341 378 4418 Fragments arranged by size: 4418 584 378 341 34 EcoRV GAT'ATC Cuts at: 2290 2290 Size: 5755 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2821 2960 2821 Size: 139 5616 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 4396 4428 4470 Size: 32 135 32 874 32 1918 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 1918 874 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 4869 5053 1305 Size: 1224 2340 184 2007 Fragments arranged by size: 2340 2007 1224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2835 3032 3713 4072 4231 518 Size: 648 1669 197 681 359 159 2042 Fragments arranged by size: 2042 1669 681 648 359 197 159 MspA1I CmG'CkG Cuts at: 1606 2152 3171 3848 4996 5241 1606 Size: 546 1019 677 1148 245 2120 Fragments arranged by size: 2120 1148 1019 677 546 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NsiI A_TGCA'T Cuts at: 2312 2312 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2593 3113 4658 1132 Size: 97 1364 520 1545 2229 Fragments arranged by size: 2229 1545 1364 520 97 PasI CC'CwG_GG Cuts at: 1348 2863 1348 Size: 1515 4240 PciI A'CATG_T Cuts at: 2589 4654 2589 Size: 2065 3690 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 1108 Size: 1561 4194 PfoI T'CCnGG_A Cuts at: 2259 2631 2259 Size: 372 5383 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2577 3107 830 Size: 1747 530 3478 Fragments arranged by size: 3478 1747 530 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2573 3103 4238 4919 5110 826 Size: 219 1528 530 1135 681 191 1471 Fragments arranged by size: 1528 1471 1135 681 530 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2806 2878 3859 4760 5022 820 Size: 197 926 863 72 981 901 262 1553 Fragments arranged by size: 1553 981 926 901 863 262 197 72 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2535 1517 Size: 1018 4737 StyI C'CwwG_G Cuts at: 513 1065 2004 3000 3194 513 Size: 552 939 996 194 3074 Fragments arranged by size: 3074 996 939 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 2437 3091 2437 Size: 654 5101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 2602 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI