(Circular) (Six-base) MAPSORT of: pFN21AE0769.seq Check: 411 from: 1 to: 5722 pFN21AE0769 5722 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2166 2832 2166 Size: 666 5056 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 3785 5169 5437 1632 Size: 647 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 647 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 2453 1166 Size: 1287 4435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 2152 2664 3016 3465 5037 2152 Size: 512 352 449 1572 2837 Fragments arranged by size: 2837 1572 512 449 352 ApaI G_GGCC'C Cuts at: 2241 2949 2241 Size: 708 5014 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 3031 3257 3409 5518 1493 Size: 1538 226 152 2109 1697 Fragments arranged by size: 2109 1697 1538 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2491 2541 2577 3047 1870 Size: 73 548 50 36 470 4545 Fragments arranged by size: 4545 548 470 73 50 36 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2251 2773 3043 4498 Size: 325 217 627 256 208 522 270 1455 Cuts at: 4498 618 Size: 1842 Fragments arranged by size: 1842 1455 627 522 325 270 256 217 208 BanII G_rGCy'C Cuts at: 1391 2093 2229 2241 2298 2949 3041 1391 Size: 702 136 12 57 651 92 4072 Fragments arranged by size: 4072 702 651 136 92 57 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2779 928 Size: 971 880 3871 Fragments arranged by size: 3871 971 880 BbvCI CC'TCA_GC Cuts at: 2981 2981 Size: 5722 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 2308 1548 Size: 760 4962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 4333 136 Size: 107 122 71 631 605 400 2261 1525 Fragments arranged by size: 2261 1525 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2048 2218 2241 2256 2413 2671 2778 2949 Size: 258 170 23 15 157 258 107 171 Cuts at: 2949 3783 4939 1790 Size: 834 1156 2573 Fragments arranged by size: 2573 1156 834 258 258 171 170 157 107 23 15 BmgBI CAC'GTC Cuts at: 1318 2692 1318 Size: 1374 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3453 4411 442 Size: 416 53 83 399 24 2036 958 1753 Fragments arranged by size: 2036 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2733 1056 Size: 1677 4045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2641 2867 4382 1239 Size: 1402 226 1515 2579 Fragments arranged by size: 2579 1515 1402 226 Bpu10I CC'TnA_GC Cuts at: 2718 2981 2718 Size: 263 5459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2344 2513 4385 882 Size: 617 845 169 1872 2219 Fragments arranged by size: 2219 1872 845 617 169 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2790 4155 4553 4827 4974 5385 2790 Size: 1365 398 274 147 411 3127 Fragments arranged by size: 3127 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5497 Fragments arranged by size: 5497 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2945 1186 Size: 1036 723 3963 Fragments arranged by size: 3963 1036 723 BseYI C'CCAG_C Cuts at: 2092 2523 2695 2722 2840 4925 5421 2092 Size: 431 172 27 118 2085 496 2393 Fragments arranged by size: 2393 2085 496 431 172 118 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2494 1163 Size: 573 402 356 4391 Fragments arranged by size: 4391 573 402 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 3172 3933 4961 1540 Size: 275 186 304 867 761 1028 2301 Fragments arranged by size: 2301 1028 867 761 304 275 186 BsiHKAI G_wGCw'C Cuts at: 2823 3783 3868 4939 2823 Size: 960 85 1071 3606 Fragments arranged by size: 3606 1071 960 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 BsmBI CGTCTCn'nnnn_ Cuts at: 2682 2682 Size: 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2093 2218 2229 2241 2256 2298 Size: 399 258 45 125 11 12 15 42 Cuts at: 2298 2413 2671 2778 2823 2949 3041 3783 3868 Size: 115 258 107 45 126 92 742 85 Cuts at: 3868 4939 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 258 258 126 125 115 107 92 85 45 45 42 15 12 11 BspEI T'CCGG_A Cuts at: 2790 5385 2790 Size: 2595 3127 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2832 3077 844 Size: 1322 666 245 3489 Fragments arranged by size: 3489 1322 666 245 BsrBI CCG'CTC Cuts at: 1394 1823 2172 2212 2490 3609 1394 Size: 429 349 40 278 1119 3507 Fragments arranged by size: 3507 1119 429 349 278 40 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2827 4366 4553 1700 Size: 110 1017 1539 187 2869 Fragments arranged by size: 2869 1539 1017 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 2596 3782 4794 2596 Size: 1186 1012 3524 Fragments arranged by size: 3524 1186 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 2763 794 Size: 1367 602 3753 Fragments arranged by size: 3753 1367 602 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2148 3052 3419 3804 3821 5273 5359 5371 Size: 176 904 367 385 17 1452 86 12 Cuts at: 5371 5717 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 904 385 367 346 176 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2018 2205 2018 Size: 187 5535 BtgI C'CryG_G Cuts at: 513 1065 2004 2084 2503 513 Size: 552 939 80 419 3732 Fragments arranged by size: 3732 939 552 419 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2383 2768 3238 4032 4052 2383 Size: 385 470 794 20 4053 Fragments arranged by size: 4053 794 470 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DraIII CAC_nnn'GTG Cuts at: 2043 2763 2043 Size: 720 5002 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2590 2734 Size: 54 1425 50 275 18 472 288 144 Cuts at: 2734 3169 4058 5687 8 Size: 435 889 1629 43 Fragments arranged by size: 1629 1425 889 472 435 288 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 3169 1537 Size: 275 490 867 4090 Fragments arranged by size: 4090 867 490 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2894 3652 1353 Size: 758 783 758 3423 Fragments arranged by size: 3423 783 758 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2641 2867 3785 4382 5169 5437 Size: 393 647 362 226 918 597 787 268 Cuts at: 5437 1239 Size: 1524 Fragments arranged by size: 1524 918 787 647 597 393 362 268 226 EcoO109I rG'GnC_Cy Cuts at: 2237 2281 2496 2582 2661 2700 2946 3013 2237 Size: 44 215 86 79 39 246 67 4946 Fragments arranged by size: 4946 246 215 86 79 67 44 39 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 FspI TGC'GCA Cuts at: 2459 2756 4228 2459 Size: 297 1472 3953 Fragments arranged by size: 3953 1472 297 FspAI rTGC'GCAy Cuts at: 2756 2756 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 4363 4395 4437 Size: 32 135 32 728 32 2031 32 42 Cuts at: 4437 4469 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 728 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 4836 5020 1305 Size: 1074 2457 184 2007 Fragments arranged by size: 2457 2007 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 10 Size: 54 1425 343 760 3140 Fragments arranged by size: 3140 1425 760 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3680 4039 4198 518 Size: 648 1287 1227 359 159 2042 Fragments arranged by size: 2042 1287 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2505 2527 2533 3138 3815 4963 5208 Size: 576 323 22 6 605 677 1148 245 Cuts at: 5208 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 605 576 323 245 22 6 NaeI GCC'GGC Cuts at: 2829 2829 Size: 5722 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4231 Fragments arranged by size: 4231 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NgoMIV G'CCGG_C Cuts at: 2827 2827 Size: 5722 NheI G'CTAG_C Cuts at: 1052 2729 1052 Size: 1677 4045 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 3080 4625 1132 Size: 97 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 97 PasI CC'CwG_GG Cuts at: 1348 2234 1348 Size: 886 4836 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 1108 Size: 1227 4495 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2871 2903 3791 3823 5304 5336 2871 Size: 32 888 32 1481 32 3257 Fragments arranged by size: 3257 1481 888 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2582 2582 Size: 5722 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PspOMI G'GGCC_C Cuts at: 2237 2945 2237 Size: 708 5014 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2909 2941 2909 Size: 32 5690 PstI C_TGCA'G Cuts at: 830 2532 3012 3074 830 Size: 1702 480 62 3478 Fragments arranged by size: 3478 1702 480 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2182 2527 2533 2182 Size: 345 6 5371 Fragments arranged by size: 5371 345 6 SacII CC_GC'GG Cuts at: 2506 2506 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SapI GCTCTTCn'nnn_ Cuts at: 2111 2111 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SexAI A'CCwGG_T Cuts at: 2039 2781 2039 Size: 742 4980 SfcI C'TryA_G Cuts at: 826 1045 2528 3008 3070 4205 4886 5077 826 Size: 219 1483 480 62 1135 681 191 1471 Fragments arranged by size: 1483 1471 1135 681 480 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 2579 3049 1872 Size: 707 470 4545 Fragments arranged by size: 4545 707 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 2549 1517 Size: 1032 4690 StyI C'CwwG_G Cuts at: 513 1065 2004 3161 513 Size: 552 939 1157 3074 Fragments arranged by size: 3074 1157 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 3749 3766 3919 4104 4549 325 Size: 1255 1134 1035 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1134 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3968 5680 96 Size: 274 80 33 51 1443 1991 1712 138 Fragments arranged by size: 1991 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 2577 3047 1870 Size: 707 470 4545 Fragments arranged by size: 4545 707 470 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI