Restriction map for
pFN21AE0760
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI Fnu4HI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI TseI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 402 GTCTAC GT^[AC][GT]AC
AflIII 398 ACATGT A^C[GA][CT]GT
Asp700I 1096 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1089 GAGCTC G[AT]GC[AT]^C
BbsI 394 GAAGAC GAAGAC(2/6)
BciVI 88 GTATCC GTATCC(6/5)
BfaI 302 CTAG C^TAG
BglII 318 AGATCT A^GATCT
BpuAI 394 GAAGAC GAAGAC(2/6)
BsaI 337 GAGACC GGTCTC(1/5)
BsiHKAI 1089 GAGCTC G[AT]GC[AT]^C
BsiYI 45 CCCAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 45 CCCAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 222 GGGAC GGGAC(10/14)
BspLU11I 398 ACATGT A^CATGT
BsrFI 99 GCCGGT [GA]^CCGG[CT]
BstYI 318 AGATCT [GA]^GATC[CT]
Cac8I 240 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 99 GCCGGT [GA]^CCGG[CT]
Cfr13I 97 GGGCC G^G[ACGT]CC
DrdI 365 GACATTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 463 CCATGG C^C[GA][CT]GG
HapII 100 CCGG C^CGG
HgaI 157 GACGC GACGC(5/10)
HpaII 100 CCGG C^CGG
MaeI 302 CTAG C^TAG
MflI 318 AGATCT [GA]^GATC[CT]
MspA1I 242 CAGCTG C[AC]G^C[GT]G
MspI 100 CCGG C^CGG
MwoI 930 GCTTATTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 463 CCATGG C^CATGG
NdeI 1136 CATATG CA^TATG
NlaIV 96 GGGGCC GG[ACGT]^[ACGT]CC
NspI 398 ACATGT [GA]CATG^[CT]
PvuII 242 CAGCTG CAG^CTG
SacI 1089 GAGCTC GAGCT^C
Sau96I 97 GGGCC G^G[ACGT]CC
SexAI 209 ACCTGGT A^CC[AT]GGT
SfaNI 939 GCATC GCATC(5/9)
SspI 345 AATATT AAT^ATT
Tsp45I 41 GTCAC ^GT[GC]AC
XhoII 318 AGATCT [GA]^GATC[CT]
XmnI 1096 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanII 274 GAGCCC G[GA]GC[CT]^C
1089 GAGCTC
BclI 162 TGATCA T^GATCA
768 TGATCA
BmyI 274 GAGCCC G[AGT]GC[ACT]^C
1089 GAGCTC
BseRI 11 CTCCTC GAGGAG(10/8)
52 GAGGAG
BsmAI 337 GAGAC GTCTC(1/5)
363 GAGAC
Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C
1089 GAGCTC
BstF5I 114 GGATG GGATG(2/0)
281 GGATG
EcoT14I 92 CCAAGG C^C[AT][AT]GG
463 CCATGG
EcoT22I 64 ATGCAT ATGCA^T
949 ATGCAT
FbaI 162 TGATCA T^GATCA
768 TGATCA
FokI 114 GGATG GGATG(9/13)
281 GGATG
MaeIII 41 GTCAC ^GT[ACGT]AC
595 GTTAC
NsiI 64 ATGCAT ATGCA^T
949 ATGCAT
SmlI 134 CTCAAG C^T[CT][GA]AG
924 CTCAAG
StyI 92 CCAAGG C^C[AT][AT]GG
463 CCATGG
TaqI 600 TCGA T^CGA
1188 TCGA
TthHB8I 600 TCGA T^CGA
1188 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsmI
BsrI BssKI BstNI DdeI DpnII
EarI EcoRI EcoRII HaeIII HinfI
HphI Ksp632I MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
Sau3AI ScrFI StuI TfiI Tru9I
Tsp509I TspRI