Restriction map for
pFN21AE0760
(Linear) (Six-base) MAPSORT of: KIEE0760.seq Check: 5787 from: 1 to: 1296
KIEE0760.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 403 1296
Size: 403 893
AflIII A'CryG_T
Cuts at: 0 398 1296
Size: 398 898
AleI CACnn'nnGTG
Cuts at: 0 704 788 1208 1296
Size: 704 84 420 88
Fragments arranged by size:
704 420 88 84
ApoI r'AATT_y
Cuts at: 0 171 198 527 779 863 1031 1199 1283
Size: 171 27 329 252 84 168 168 84
Cuts at: 1283 1296
Size: 13
Fragments arranged by size:
329 252 171 168 168 84 84 27
13
BanII G_rGCy'C
Cuts at: 0 278 1093 1296
Size: 278 815 203
Fragments arranged by size:
815 278 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 1296
Size: 401 895
BciVI GTATCCnnnnn_n'
Cuts at: 0 99 1296
Size: 99 1197
BclI T'GATC_A
Cuts at: 0 162 768 1296
Size: 162 606 528
Fragments arranged by size:
606 528 162
BfrBI ATG'CAT
Cuts at: 0 66 951 1296
Size: 66 885 345
Fragments arranged by size:
885 345 66
BglII A'GATC_T
Cuts at: 0 318 1296
Size: 318 978
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 909 1296
Size: 119 790 387
Fragments arranged by size:
790 387 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 1296
Size: 331 965
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2 67 1296
Size: 2 65 1229
Fragments arranged by size:
1229 65 2
BsiHKAI G_wGCw'C
Cuts at: 0 1093 1296
Size: 1093 203
BsmI GAATG_Cn'
Cuts at: 0 68 154 953 1296
Size: 68 86 799 343
Fragments arranged by size:
799 343 86 68
Bsp1286I G_dGCh'C
Cuts at: 0 278 1093 1296
Size: 278 815 203
Fragments arranged by size:
815 278 203
BsrFI r'CCGG_y
Cuts at: 0 99 1296
Size: 99 1197
BstYI r'GATC_y
Cuts at: 0 318 1296
Size: 318 978
BtgI C'CryG_G
Cuts at: 0 463 1296
Size: 463 833
BtsI GCAGTG_nn'
Cuts at: 0 988 1296
Size: 988 308
DrdI GACnn_nn'nnGTC
Cuts at: 0 371 1296
Size: 371 925
EarI CTCTTCn'nnn_
Cuts at: 0 44 355 449 1296
Size: 44 311 94 847
Fragments arranged by size:
847 311 94 44
EcoICRI GAG'CTC
Cuts at: 0 1091 1296
Size: 1091 205
EcoRI G'AATT_C
Cuts at: 0 527 779 863 1031 1199 1283 1296
Size: 527 252 84 168 168 84 13
Fragments arranged by size:
527 252 168 168 84 84 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 480 512 1296
Size: 310 32 138 32 784
Fragments arranged by size:
784 310 138 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 540 1296
Size: 173 367 756
Fragments arranged by size:
756 367 173
MslI CAynn'nnrTG
Cuts at: 0 468 536 704 788 872 956 1040 1124
Size: 468 68 168 84 84 84 84 84
Cuts at: 1124 1140 1208 1296
Size: 16 68 88
Fragments arranged by size:
468 168 88 84 84 84 84 84
68 68 16
MspA1I CmG'CkG
Cuts at: 0 244 1296
Size: 244 1052
NcoI C'CATG_G
Cuts at: 0 463 1296
Size: 463 833
NdeI CA'TA_TG
Cuts at: 0 1137 1296
Size: 1137 159
NsiI A_TGCA'T
Cuts at: 0 68 953 1296
Size: 68 885 343
Fragments arranged by size:
885 343 68
NspI r_CATG'y
Cuts at: 0 402 1296
Size: 402 894
PciI A'CATG_T
Cuts at: 0 398 1296
Size: 398 898
PvuII CAG'CTG
Cuts at: 0 244 1296
Size: 244 1052
SacI G_AGCT'C
Cuts at: 0 1093 1296
Size: 1093 203
SexAI A'CCwGG_T
Cuts at: 0 209 1296
Size: 209 1087
SmlI C'TyrA_G
Cuts at: 0 134 924 1296
Size: 134 790 372
Fragments arranged by size:
790 372 134
SspI AAT'ATT
Cuts at: 0 347 1296
Size: 347 949
StuI AGG'CCT
Cuts at: 0 308 994 1078 1162 1296
Size: 308 686 84 84 134
Fragments arranged by size:
686 308 134 84 84
StyI C'CwwG_G
Cuts at: 0 92 463 1296
Size: 92 371 833
Fragments arranged by size:
833 371 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 1296
Size: 59 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 497 529 1296
Size: 316 32 149 32 767
Fragments arranged by size:
767 316 149 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1100 1296
Size: 1100 196
Enzymes that do cut:
AccI AflIII AleI ApoI BanII BbsI BciVI BclI
BfrBI BglII BpuEI BsaI BseRI BsiHKAI BsmI Bsp1286I
BsrFI BstYI BtgI BtsI DrdI EarI EcoICRI EcoRI
Hin4I MmeI MslI MspA1I NcoI NdeI NsiI NspI
PciI PvuII SacI SexAI SmlI SspI StuI StyI
TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
ZraI