Restriction map for
pFN21AE0759
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 130 AAATTT [GA]^AATT[CT]
AfaI 801 GTAC GT^AC
AflIII 419 ACGTGT A^C[GA][CT]GT
Alw44I 887 GTGCAC G^TGCAC
ApaLI 887 GTGCAC G^TGCAC
ApoI 130 AAATTT [GA]^AATT[CT]
AseI 708 ATTAAT AT^TAAT
AsnI 708 ATTAAT AT^TAAT
Asp700I 127 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 887 GTGCAC G[AT]GC[AT]^C
AspI 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 215 CCCGAG C^[CT]CG[GA]G
BalI 683 TGGCCA TGG^CCA
BanII 367 GAGCCC G[GA]GC[CT]^C
BbsI 267 GAAGAC GAAGAC(2/6)
BcnI 734 CCCGG CC^[GC]GG
BlpI 691 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 691 GCTGAGC GC^T[ACGT]AGC
BpuAI 267 GAAGAC GAAGAC(2/6)
BsaAI 37 TACGTG [CT]AC^GT[GA]
BsiEI 561 CGGTCG CG[GA][CT]^CG
BsiHKAI 887 GTGCAC G[AT]GC[AT]^C
BsmI 460 GAATGC GAATGC(1/-1)
BsoBI 215 CCCGAG C^[CT]CG[GA]G
BspHI 480 TCATGA T^CATGA
BssSI 884 CTCGTG CACGAG(-5/-1)
BstF5I 987 CATCC GGATG(2/0)
CelII 691 GCTGAGC GC^T[ACGT]AGC
CfoI 797 GCGC GCG^C
DraII 111 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 111 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 502 GATATC GAT^ATC
FauI 396 GCGGG CCCGC(4/6)
FokI 987 CATCC GGATG(9/13)
HapII 735 CCGG C^CGG
HhaI 797 GCGC GCG^C
HinP1I 797 GCGC G^CGC
HpaII 735 CCGG C^CGG
MluNI 683 TGGCCA TGG^CCA
MscI 683 TGGCCA TGG^CCA
MspA1I 394 CAGCGG C[AC]G^C[GT]G
MspI 735 CCGG C^CGG
NciI 734 CCCGG CC^[GC]GG
NspI 231 GCATGC [GA]CATG^[CT]
PflFI 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 111 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 708 ATTAAT AT^TAAT
RcaI 480 TCATGA T^CATGA
RsaI 801 GTAC GT^AC
SphI 231 GCATGC GCATG^C
TaqI 265 TCGA T^CGA
Tsp45I 605 GTGAC ^GT[GC]AC
Tsp509I 131 AATT ^AATT
Tth111I 19 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 265 TCGA T^CGA
XcmI 515 CCAAGGTCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 127 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 798 CGCG CG^CG
947 CGCG
BfaI 104 CTAG C^TAG
715 CTAG
BmyI 367 GAGCCC G[AGT]GC[ACT]^C
887 GTGCAC
BpmI 978 CTCCAG CTGGAG(16/14)
292 CTGGAG
BseRI 301 GAGGAG GAGGAG(10/8)
552 GAGGAG
BsgI 184 CTGCAC GTGCAG(16/14)
391 GTGCAG
BsiYI 299 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCTCTGCATGG
BslI 299 CCGAGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
318 CCTCTGCATGG
BsmAI 220 GAGAC GTCTC(1/5)
882 GTCTC
BsmFI 337 GGGAC GGGAC(10/14)
566 GGGAC
Bsp1286I 367 GAGCCC G[AGT]GC[ACT]^C
887 GTGCAC
BstNI 341 CCAGG CC^[AT]GG
444 CCTGG
BstUI 798 CGCG CG^CG
947 CGCG
Cac8I 231 GCATGC GC[ACGT]^[ACGT]GC
311 GCAAGC
DpnII 474 GATC ^GATC
654 GATC
EaeI 380 TGGCCG [CT]^GGCC[GA]
683 TGGCCA
EarI 785 CTCTTC CTCTTC(1/4)
925 GAAGAG
EcoRII 341 CCAGG ^CC[AT]GG
444 CCTGG
EcoT14I 360 CCTTGG C^C[AT][AT]GG
515 CCAAGG
HindIII 58 AAGCTT A^AGCTT
747 AAGCTT
Ksp632I 785 CTCTTC CTCTTC(1/4)
925 GAAGAG
MaeI 104 CTAG C^TAG
715 CTAG
MaeIII 175 GTAAC ^GT[ACGT]AC
605 GTGAC
MboI 474 GATC ^GATC
654 GATC
MseI 14 TTAA T^TAA
709 TTAA
MvaI 341 CCAGG CC^[AT]GG
444 CCTGG
MvnI 798 CGCG CG^CG
947 CGCG
MwoI 676 GCGACACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
939 GCCGTTGTCGC
NdeII 474 GATC ^GATC
654 GATC
Sau3AI 474 GATC ^GATC
654 GATC
SfaNI 77 GATGC GCATC(5/9)
807 GCATC
SfcI 843 CTACAG C^T[GA][CT]AG
972 CTACAG
StyI 360 CCTTGG C^C[AT][AT]GG
515 CCAAGG
TfiI 413 GATTC G^A[AT]TC
965 GATTC
Tru9I 14 TTAA T^TAA
709 TTAA
TspRI 548 CACTG CA[GC]TG[ACGT][ACGT]^
680 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsrI BssKI Cfr13I DdeI Fnu4HI
HaeIII HinfI HphI ItaI MaeII
MboII MnlI NlaIII NlaIV PleI
Sau96I ScrFI TseI