Restriction map for
pFN21AE0759
(Linear) (Six-base) MAPSORT of: KIEE0759.seq Check: 4868 from: 1 to: 993
KIEE0759.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 993
Size: 724 269
AflIII A'CryG_T
Cuts at: 0 419 993
Size: 419 574
ApaLI G'TGCA_C
Cuts at: 0 887 993
Size: 887 106
ApoI r'AATT_y
Cuts at: 0 130 993
Size: 130 863
AseI AT'TA_AT
Cuts at: 0 709 993
Size: 709 284
AvaI C'yCGr_G
Cuts at: 0 215 993
Size: 215 778
BanII G_rGCy'C
Cuts at: 0 371 993
Size: 371 622
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 993
Size: 274 719
BlpI GC'TnA_GC
Cuts at: 0 692 993
Size: 692 301
Bme1580I G_kGCm'C
Cuts at: 0 891 993
Size: 891 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 963 993
Size: 313 650 30
Fragments arranged by size:
650 313 30
BsaAI yAC'GTr
Cuts at: 0 39 993
Size: 39 954
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 546 576 993
Size: 546 30 417
Fragments arranged by size:
546 417 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 567 993
Size: 316 251 426
Fragments arranged by size:
426 316 251
BseYI C'CCAG_C
Cuts at: 0 901 932 993
Size: 901 31 61
Fragments arranged by size:
901 61 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 412 993
Size: 169 243 581
Fragments arranged by size:
581 243 169
BsiEI CG_ry'CG
Cuts at: 0 564 993
Size: 564 429
BsiHKAI G_wGCw'C
Cuts at: 0 891 993
Size: 891 102
BsmI GAATG_Cn'
Cuts at: 0 466 993
Size: 466 527
Bsp1286I G_dGCh'C
Cuts at: 0 371 891 993
Size: 371 520 102
Fragments arranged by size:
520 371 102
BspHI T'CATG_A
Cuts at: 0 480 993
Size: 480 513
BssSI C'ACGA_G
Cuts at: 0 884 993
Size: 884 109
EaeI y'GGCC_r
Cuts at: 0 380 683 993
Size: 380 303 310
Fragments arranged by size:
380 310 303
EarI CTCTTCn'nnn_
Cuts at: 0 791 920 993
Size: 791 129 73
Fragments arranged by size:
791 129 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 724 963 993
Size: 313 411 239 30
Fragments arranged by size:
411 313 239 30
EcoO109I rG'GnC_Cy
Cuts at: 0 112 993
Size: 112 881
EcoRV GAT'ATC
Cuts at: 0 504 993
Size: 504 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 522 546 554 578 638 670
Size: 247 32 243 24 8 24 60 32
Cuts at: 670 993
Size: 323
Fragments arranged by size:
323 247 243 60 32 32 24 24
8
HindIII A'AGCT_T
Cuts at: 0 58 747 993
Size: 58 689 246
Fragments arranged by size:
689 246 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 993
Size: 32 961
MscI TGG'CCA
Cuts at: 0 685 993
Size: 685 308
MspA1I CmG'CkG
Cuts at: 0 396 993
Size: 396 597
NspI r_CATG'y
Cuts at: 0 235 993
Size: 235 758
PpuMI rG'GwC_Cy
Cuts at: 0 112 993
Size: 112 881
SfcI C'TryA_G
Cuts at: 0 843 972 993
Size: 843 129 21
Fragments arranged by size:
843 129 21
SphI G_CATG'C
Cuts at: 0 235 993
Size: 235 758
StyI C'CwwG_G
Cuts at: 0 360 515 993
Size: 360 155 478
Fragments arranged by size:
478 360 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 993
Size: 559 32 402
Fragments arranged by size:
559 402 32
Tth111I GACn'n_nGTC
Cuts at: 0 22 993
Size: 22 971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 993
Size: 522 471
XmnI GAAnn'nnTTC
Cuts at: 0 131 993
Size: 131 862
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AseI AvaI BanII BbsI
BlpI Bme1580I BpmI BsaAI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BssSI EaeI EarI
Eco57MI EcoO109I EcoRV Hin4I HindIII MmeI MscI MspA1I
NspI PpuMI SfcI SphI StyI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI XbaI
XhoI XmaI ZraI