(Circular) (Six-base) MAPSORT of: pFN21AE0749.seq Check: 8655 from: 1 to: 5683 pFN21AE0749 5683 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2452 2452 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 2604 3026 1937 Size: 229 438 422 4594 Fragments arranged by size: 4594 438 422 229 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 2627 1166 Size: 1461 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2467 2809 3426 4998 2467 Size: 342 617 1572 3152 Fragments arranged by size: 3152 1572 617 342 ApaI G_GGCC'C Cuts at: 2049 2049 Size: 5683 ApaLI G'TGCA_C Cuts at: 2504 2929 3740 4896 2504 Size: 425 811 1156 3291 Fragments arranged by size: 3291 1156 811 425 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AscI GG'CGCG_CC Cuts at: 2444 2444 Size: 5683 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2381 2625 3008 1870 Size: 73 438 244 383 4545 Fragments arranged by size: 4545 438 383 244 73 BamHI G'GATC_C Cuts at: 2649 3013 3380 2649 Size: 364 367 4952 Fragments arranged by size: 4952 367 364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2621 2820 3004 4459 Size: 325 217 627 636 198 199 184 1455 Cuts at: 4459 618 Size: 1842 Fragments arranged by size: 1842 1455 636 627 325 217 199 198 184 BanII G_rGCy'C Cuts at: 1391 2049 2196 2559 3002 1391 Size: 658 147 363 443 4072 Fragments arranged by size: 4072 658 443 363 147 BbeI G_GCGC'C Cuts at: 2427 2427 Size: 5683 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3874 3908 1903 Size: 34 50 34 1853 34 3678 Fragments arranged by size: 3678 1853 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 4294 136 Size: 107 122 71 631 605 581 2041 1525 Fragments arranged by size: 2041 1525 631 605 581 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2049 2268 2508 2933 3744 4900 1790 Size: 259 219 240 425 811 1156 2573 Fragments arranged by size: 2573 1156 811 425 259 240 219 BmgBI CAC'GTC Cuts at: 1318 2950 2964 1318 Size: 1632 14 4037 Fragments arranged by size: 4037 1632 14 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2808 2808 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2393 2778 4343 1239 Size: 1052 102 385 1565 2579 Fragments arranged by size: 2579 1565 1052 385 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 2576 3808 4673 4971 2543 Size: 33 1232 865 298 3255 Fragments arranged by size: 3255 1232 865 298 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2794 4346 882 Size: 617 1295 1552 2219 Fragments arranged by size: 2219 1552 1295 617 BsaAI yAC'GTr Cuts at: 493 1204 2215 3474 493 Size: 711 1011 1259 2702 Fragments arranged by size: 2702 1259 1011 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 2424 2901 3490 Size: 53 83 186 936 786 105 477 589 Cuts at: 3490 3872 4507 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 786 635 589 477 382 186 105 83 53 BsaWI w'CCGG_w Cuts at: 2549 2778 2814 4116 4514 4788 4935 5346 2549 Size: 229 36 1302 398 274 147 411 2886 Fragments arranged by size: 2886 1302 411 398 274 229 147 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 1178 Size: 30 165 30 1128 30 4300 Fragments arranged by size: 4300 1128 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2765 2797 1186 Size: 1579 32 4072 Fragments arranged by size: 4072 1579 32 BseYI C'CCAG_C Cuts at: 2140 2695 2884 4886 5382 2140 Size: 555 189 2002 496 2441 Fragments arranged by size: 2441 2002 555 496 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2262 2849 3133 3894 4922 1540 Size: 275 186 261 587 284 761 1028 2301 Fragments arranged by size: 2301 1028 761 587 284 275 261 186 BsiHKAI G_wGCw'C Cuts at: 2508 2559 2933 3744 3829 4900 2508 Size: 51 374 811 85 1071 3291 Fragments arranged by size: 3291 1071 811 374 85 51 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2196 2268 2508 2559 2933 3002 Size: 399 259 147 72 240 51 374 69 Cuts at: 3002 3744 3829 4900 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 259 240 147 85 72 69 51 BspEI T'CCGG_A Cuts at: 2549 2778 5346 2549 Size: 229 2568 2886 Fragments arranged by size: 2886 2568 229 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 2550 2928 3038 844 Size: 1608 98 378 110 3489 Fragments arranged by size: 3489 1608 378 110 98 BsrBI CCG'CTC Cuts at: 1394 1823 2227 2347 2377 2845 3570 1394 Size: 429 404 120 30 468 725 3507 Fragments arranged by size: 3507 725 468 429 404 120 30 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2386 4327 4514 1700 Size: 110 576 1941 187 2869 Fragments arranged by size: 2869 1941 576 187 110 BsrGI T'GTAC_A Cuts at: 96 2631 96 Size: 2535 3148 BssHII G'CGCG_C Cuts at: 1925 2310 2312 2444 1925 Size: 385 2 132 5164 Fragments arranged by size: 5164 385 132 2 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 4637 BstYI r'GATC_y Cuts at: 1972 2076 2649 2754 3013 3380 3765 3782 5234 Size: 104 573 105 259 367 385 17 1452 Cuts at: 5234 5320 5332 5678 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 573 385 367 346 259 105 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2668 513 Size: 552 1603 3528 Fragments arranged by size: 3528 1603 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2167 487 Size: 683 56 941 4003 Fragments arranged by size: 4003 941 683 56 BtsI GCAGTG_nn' Cuts at: 2142 2482 3199 3993 4013 2142 Size: 340 717 794 20 3812 Fragments arranged by size: 3812 794 717 340 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DraIII CAC_nnn'GTG Cuts at: 2218 2688 2218 Size: 470 5213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2900 4690 809 Size: 507 1584 1790 1802 Fragments arranged by size: 1802 1790 1584 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 2679 2846 Size: 54 1425 50 275 18 639 210 167 Cuts at: 2846 2952 3130 4019 5648 8 Size: 106 178 889 1629 43 Fragments arranged by size: 1629 1425 889 639 275 210 178 167 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2846 3130 1537 Size: 275 1034 284 4090 Fragments arranged by size: 4090 1034 284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2780 3613 1353 Size: 1052 375 833 3423 Fragments arranged by size: 3423 1052 833 375 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 2315 4270 4644 4790 387 Size: 1064 138 522 204 1955 374 146 1280 Fragments arranged by size: 1955 1280 1064 522 374 204 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2393 2778 3746 4343 5130 5398 Size: 393 659 102 385 968 597 787 268 Cuts at: 5398 1239 Size: 1524 Fragments arranged by size: 1524 968 787 659 597 393 385 268 102 EcoO109I rG'GnC_Cy Cuts at: 2395 2395 Size: 5683 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2125 Size: 5683 FspI TGC'GCA Cuts at: 2288 4189 2288 Size: 1901 3782 HaeII r_GCGC'y Cuts at: 1678 2427 2677 4830 1678 Size: 749 250 2153 2531 Fragments arranged by size: 2531 2153 749 250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 2834 2866 4324 Size: 32 135 32 959 32 271 32 1458 Cuts at: 4324 4356 4398 4430 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1458 959 271 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KasI G'GCGC_C Cuts at: 2423 2423 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 10 Size: 54 1425 343 1122 2739 Fragments arranged by size: 2739 1425 1122 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2627 3641 4000 4159 518 Size: 648 1461 1014 359 159 2042 Fragments arranged by size: 2042 1461 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2440 2493 2809 3099 3776 4924 5169 1606 Size: 834 53 316 290 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 677 316 290 245 53 NaeI GCC'GGC Cuts at: 2388 2388 Size: 5683 NarI GG'CG_CC Cuts at: 2424 2424 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5131 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2386 2386 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 2688 1108 Size: 1580 4103 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PmlI CAC'GTG Cuts at: 2215 2215 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2395 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2045 2045 Size: 5683 PstI C_TGCA'G Cuts at: 830 2085 3035 830 Size: 1255 950 3478 Fragments arranged by size: 3478 1255 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2493 2809 2493 Size: 316 5367 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SapI GCTCTTCn'nnn_ Cuts at: 2780 2780 Size: 5683 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SexAI A'CCwGG_T Cuts at: 2124 2124 Size: 5683 SfcI C'TryA_G Cuts at: 826 1045 2081 3031 4166 4847 5038 826 Size: 219 1036 950 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 950 681 219 191 SfoI GGC'GCC Cuts at: 2425 2425 Size: 5683 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 2591 3787 4688 4950 820 Size: 197 926 615 33 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 615 262 197 33 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 2091 3122 513 Size: 552 1026 1031 3074 Fragments arranged by size: 3074 1031 1026 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 3710 3727 3880 4065 4510 325 Size: 1255 669 1461 17 153 185 445 1498 Fragments arranged by size: 1498 1461 1255 669 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 3929 5641 Size: 274 80 33 51 1443 654 1298 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1443 1298 654 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 2600 1316 Size: 1284 4399 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI