Restriction map for
pFN21AE0742
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 1042 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 28 CCCGAG C^[CT]CG[GA]G
BbrPI 275 CACGTG CAC^GTG
BbsI 762 GAAGAC GAAGAC(2/6)
BfaI 965 CTAG C^TAG
BpmI 1019 CTGGAG CTGGAG(16/14)
BpuAI 762 GAAGAC GAAGAC(2/6)
BsaBI 702 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 617 TCCGGT [AT]^CCGG[AT]
BseRI 1137 CTCCTC GAGGAG(10/8)
BsoBI 28 CCCGAG C^[CT]CG[GA]G
BspLU11I 836 ACATGT A^CATGT
BsrFI 917 ACCGGC [GA]^CCGG[CT]
BstAPI 597 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 824 CCAACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 917 ACCGGC [GA]^CCGG[CT]
DraIII 1208 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1057 CCGTGG C^C[GA][CT]GG
EarI 1080 CTCTTC CTCTTC(1/4)
EcoNI 978 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 843 GGCGCT [GA]GCGC^[CT]
Ksp632I 1080 CTCTTC CTCTTC(1/4)
MaeI 965 CTAG C^TAG
MamI 702 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1174 ACGCGT A^CGCGT
MseI 1127 TTAA T^TAA
PflMI 468 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 275 CACGTG CAC^GTG
PmlI 275 CACGTG CAC^GTG
PpuMI 20 AGGTCCT [GA]G^G[AT]CC[CT]
SphI 627 GCATGC GCATG^C
TaqI 485 TCGA T^CGA
TfiI 666 GAATC G^A[AT]TC
Tru9I 1127 TTAA T^TAA
Tsp509I 309 AATT ^AATT
TthHB8I 485 TCGA T^CGA
Van91I 468 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 924 GATCC GGATC(4/5)
54 GGATC
AocI 927 CCTCAGG CC^T[ACGT]AGG
1047 CCTGAGG
BanI 877 GGCACC G^G[CT][GA]CC
1052 GGCACC
BsaAI 275 CACGTG [CT]AC^GT[GA]
338 TACGTG
BsaI 388 GAGACC GGTCTC(1/5)
650 GAGACC
BsgI 1034 CTGCAC GTGCAG(16/14)
1099 CTGCAC
BsmFI 10 GGGAC GGGAC(10/14)
191 GTCCC
Bsp1407I 101 TGTACA T^GTACA
503 TGTACA
BspMI 1097 ACCTGC ACCTGC(4/8)
1244 ACCTGC
BsrGI 101 TGTACA T^GTACA
503 TGTACA
BstYI 54 GGATCT [GA]^GATC[CT]
923 AGATCC
Bsu36I 927 CCTCAGG CC^T[ACGT]AGG
1047 CCTGAGG
Eco81I 927 CCTCAGG CC^T[ACGT]AGG
1047 CCTGAGG
MflI 54 GGATCT [GA]^GATC[CT]
923 AGATCC
MspA1I 49 CAGCTG C[AC]G^C[GT]G
469 CAGCTG
NspI 627 GCATGC [GA]CATG^[CT]
836 ACATGT
PleI 694 GACTC GAGTC(4/5)
421 GAGTC
PstI 595 CTGCAG CTGCA^G
1039 CTGCAG
PvuII 49 CAGCTG CAG^CTG
469 CAGCTG
SacI 535 GAGCTC GAGCT^C
1237 GAGCTC
SfaNI 548 GCATC GCATC(5/9)
638 GCATC
SfcI 595 CTGCAG C^T[GA][CT]AG
1039 CTGCAG
SspBI 101 TGTACA T^GTACA
503 TGTACA
XhoII 54 GGATCT [GA]^GATC[CT]
923 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AspHI AvaII BalI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI