Restriction map for
pFN21AE0742
(Circular) (Six-base) MAPSORT of: pFN21AE0742.seq Check: 9450 from: 1 to: 5977
pFN21AE0742 5977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2314 4040 5424 5692 1632
Size: 436 246 1726 1384 268 1917
Fragments arranged by size:
1917 1726 1384 436 268 246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2103 2281 2344 2841 3179 4876 5853 1203
Size: 900 178 63 497 338 1697 977 1327
Fragments arranged by size:
1697 1327 977 900 497 338 178 63
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2126 2594 1166
Size: 960 468 4549
Fragments arranged by size:
4549 960 468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2203 2391 1184
Size: 1019 188 4770
Fragments arranged by size:
4770 1019 188
AlwNI CAG_nnn'CTG
Cuts at: 3052 3720 5292 3052
Size: 668 1572 3737
Fragments arranged by size:
3737 1572 668
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 3302 1870
Size: 73 90 1269 4545
Fragments arranged by size:
4545 1269 90 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2893 2926 2893
Size: 33 5944
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2882 3057 3298 4753 618
Size: 325 217 627 1095 175 241 1455 1842
Fragments arranged by size:
1842 1455 1095 627 325 241 217 175
BanII G_rGCy'C
Cuts at: 1391 2082 2544 2570 2964 3031 3246 3296 1391
Size: 691 462 26 394 67 215 50 4072
Fragments arranged by size:
4072 691 462 394 215 67 50 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2774 928
Size: 971 875 4131
Fragments arranged by size:
4131 971 875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2696 4038 5194 1790
Size: 906 1342 1156 2573
Fragments arranged by size:
2573 1342 1156 906
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 5191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 3166 3708
Size: 416 53 83 399 24 1231 518 542
Cuts at: 3708 4666 442
Size: 958 1753
Fragments arranged by size:
1753 1231 958 542 518 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3045 4637 1239
Size: 1806 1592 2579
Fragments arranged by size:
2579 1806 1592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 2649 4640 882
Size: 617 888 262 1991 2219
Fragments arranged by size:
2219 1991 888 617 262
BsaAI yAC'GTr
Cuts at: 493 1204 2282 2345 3768 493
Size: 711 1078 63 1423 2702
Fragments arranged by size:
2702 1423 1078 711 63
BsaBI GATnn'nnATC
Cuts at: 2711 5971 2711
Size: 3260 2717
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 2619 2856 3784
Size: 53 83 186 936 1053 33 237 928
Cuts at: 3784 4166 4801 275
Size: 382 635 1451
Fragments arranged by size:
1451 1053 936 928 635 382 237 186
83 53 33
BsaWI w'CCGG_w
Cuts at: 2622 4410 4808 5082 5229 5640 2622
Size: 1788 398 274 147 411 2959
Fragments arranged by size:
2959 1788 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2206 2236 2492 2522 1178
Size: 30 165 30 803 30 256 30 4633
Fragments arranged by size:
4633 803 256 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3133 1186
Size: 1947 4030
BseYI C'CCAG_C
Cuts at: 2161 2851 5180 5676 2161
Size: 690 2329 496 2462
Fragments arranged by size:
2462 2329 690 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3024 3089 1163
Size: 573 1288 65 4051
Fragments arranged by size:
4051 1288 573 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 2544 2740 3161 3246 4038 4123 5194 2355
Size: 189 196 421 85 792 85 1071 3138
Fragments arranged by size:
3138 1071 792 421 196 189 85 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2355 2544 2570 2696 2740 2964
Size: 399 292 273 189 26 126 44 224
Cuts at: 2964 3031 3161 3246 3296 4038 4123 5194 1391
Size: 67 130 85 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 292 273 224 189
130 126 85 85 67 50 44 26
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3111 3258 3332 844
Size: 2267 147 74 3489
Fragments arranged by size:
3489 2267 147 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2503 3111 3192 3864 1394
Size: 429 680 608 81 672 3507
Fragments arranged by size:
3507 680 672 608 429 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 2556 2793 2859 4472 4646 65
Size: 1183 1308 237 66 1613 174 1396
Fragments arranged by size:
1613 1396 1308 1183 237 174 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 2922 4621 4808 1700
Size: 110 1112 1699 187 2869
Fragments arranged by size:
2869 1699 1112 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 2508 96
Size: 2010 402 3565
Fragments arranged by size:
3565 2010 402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2608 794
Size: 1814 4163
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 2836
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2059 2928 3307 3674 4059 4076 5528 5614
Size: 87 869 379 367 385 17 1452 86
Cuts at: 5614 5626 5972 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 869 385 379 367 346 87
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2933 3053 2933
Size: 120 5857
BtgI C'CryG_G
Cuts at: 513 1065 2004 3062 513
Size: 552 939 1058 3428
Fragments arranged by size:
3428 1058 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3041 3493 4287 4307 3041
Size: 452 794 20 4711
Fragments arranged by size:
4711 794 452 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 3218 3218
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2317 2526
Size: 54 1425 50 275 18 379 108 209
Cuts at: 2526 2730 2985 3424 4313 5942 8
Size: 204 255 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 379 275 255 209
204 108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3091 3907 1353
Size: 1738 816 3423
Fragments arranged by size:
3423 1738 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 3244 2542
Size: 702 5275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2314 3045 4040 4637 5424 5692
Size: 393 436 246 731 995 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 995 787 731 597 436 393 268
246
EcoNI CCTnn'n_nnAGG
Cuts at: 2987 2987
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 2026 2308 3049 2026
Size: 282 741 4954
Fragments arranged by size:
4954 741 282
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2852 5124 1678
Size: 1174 2272 2531
Fragments arranged by size:
2531 2272 1174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4618 4650 4692 4724 1373
Size: 32 135 32 3046 32 42 32 2626
Fragments arranged by size:
3046 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 3179 5853 3179
Size: 2674 3303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 3003 5091 5275 1305
Size: 1104 594 2088 184 2007
Fragments arranged by size:
2088 2007 1104 594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2211 2528 2732 2987 10
Size: 54 1425 343 379 317 204 255 3000
Fragments arranged by size:
3000 1425 379 343 317 255 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2384 2516 2594 3935 4294 4453
Size: 648 960 258 132 78 1341 359 159
Cuts at: 4453 518
Size: 2042
Fragments arranged by size:
2042 1341 960 648 359 258 159 132
78
MspA1I CmG'CkG
Cuts at: 1606 2056 2476 3393 4070 5218 5463 1606
Size: 450 420 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 450 420 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2636 2845 3335 4880 1132
Size: 97 1407 209 490 1545 2229
Fragments arranged by size:
2229 1545 1407 490 209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2841 4876 2841
Size: 2035 3942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4606
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 2282 2282
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2026
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2604 3048 3329 830
Size: 1774 444 281 3478
Fragments arranged by size:
3478 1774 444 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2056 2476 2056
Size: 420 5557
SacI G_AGCT'C
Cuts at: 2544 3246 2544
Size: 702 5275
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2600 3044 3325 4460 5141 5332 826
Size: 219 1555 444 281 1135 681 191 1471
Fragments arranged by size:
1555 1471 1135 681 444 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 2636 3335 1132
Size: 1504 699 3774
Fragments arranged by size:
3774 1504 699
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2562 2988 3416 513
Size: 552 939 39 519 426 428 3074
Fragments arranged by size:
3074 939 552 519 428 426 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2056 3055 4004 4021 4174 4359 4804
Size: 1255 476 999 949 17 153 185 445
Cuts at: 4804 325
Size: 1498
Fragments arranged by size:
1498 1255 999 949 476 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2259 2508
Size: 274 80 33 51 1443 129 153 249
Cuts at: 2508 2790 2943 4223 5935 96
Size: 282 153 1280 1712 138
Fragments arranged by size:
1712 1443 1280 282 274 249 153 153
138 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 5937 5969 2206
Size: 32 3699 32 2214
Fragments arranged by size:
3699 2214 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI