Restriction map for
pFN21AE0742
(Linear) (Six-base) MAPSORT of: KIEE0742.seq Check: 7802 from: 1 to: 1272
KIEE0742.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 309 1272
Size: 63 246 963
Fragments arranged by size:
963 246 63
AflIII A'CryG_T
Cuts at: 0 98 276 339 836 1174 1272
Size: 98 178 63 497 338 98
Fragments arranged by size:
497 338 178 98 98 63
AleI CACnn'nnGTG
Cuts at: 0 121 589 1272
Size: 121 468 683
Fragments arranged by size:
683 468 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 386 1272
Size: 198 188 886
Fragments arranged by size:
886 198 188
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1272
Size: 1047 225
AvaI C'yCGr_G
Cuts at: 0 28 1272
Size: 28 1244
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 888 921 1272
Size: 888 33 351
Fragments arranged by size:
888 351 33
BanI G'GyrC_C
Cuts at: 0 877 1052 1272
Size: 877 175 220
Fragments arranged by size:
877 220 175
BanII G_rGCy'C
Cuts at: 0 77 539 565 959 1026 1241 1272
Size: 77 462 26 394 67 215 31
Fragments arranged by size:
462 394 215 77 67 31 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 769 1272
Size: 769 503
Bme1580I G_kGCm'C
Cuts at: 0 691 1272
Size: 691 581
BmgBI CAC'GTC
Cuts at: 0 99 1272
Size: 99 1173
BmrI ACTGGGnnnn_n'
Cuts at: 0 643 1161 1272
Size: 643 518 111
Fragments arranged by size:
643 518 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 1272
Size: 237 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1272
Size: 1040 232
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 644 1272
Size: 382 262 628
Fragments arranged by size:
628 382 262
BsaAI yAC'GTr
Cuts at: 0 277 340 1272
Size: 277 63 932
Fragments arranged by size:
932 277 63
BsaBI GATnn'nnATC
Cuts at: 0 706 1272
Size: 706 566
BsaHI Gr'CG_yC
Cuts at: 0 581 614 851 1272
Size: 581 33 237 421
Fragments arranged by size:
581 421 237 33
BsaWI w'CCGG_w
Cuts at: 0 617 1272
Size: 617 655
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 201 231 487 517 1272
Size: 201 30 256 30 755
Fragments arranged by size:
755 256 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1128 1272
Size: 1128 144
BseYI C'CCAG_C
Cuts at: 0 156 846 1272
Size: 156 690 426
Fragments arranged by size:
690 426 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1084 1272
Size: 1019 65 188
Fragments arranged by size:
1019 188 65
BsiHKAI G_wGCw'C
Cuts at: 0 350 539 735 1156 1241 1272
Size: 350 189 196 421 85 31
Fragments arranged by size:
421 350 196 189 85 31
Bsp1286I G_dGCh'C
Cuts at: 0 77 350 539 565 691 735 959 1026
Size: 77 273 189 26 126 44 224 67
Cuts at: 1026 1156 1241 1272
Size: 130 85 31
Fragments arranged by size:
273 224 189 130 126 85 77 67
44 31 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1106 1253 1272
Size: 1106 147 19
Fragments arranged by size:
1106 147 19
BsrBI CCG'CTC
Cuts at: 0 498 1106 1187 1272
Size: 498 608 81 85
Fragments arranged by size:
608 498 85 81
BsrDI GCAATG_nn'
Cuts at: 0 551 788 854 1272
Size: 551 237 66 418
Fragments arranged by size:
551 418 237 66
BsrFI r'CCGG_y
Cuts at: 0 917 1272
Size: 917 355
BsrGI T'GTAC_A
Cuts at: 0 101 503 1272
Size: 101 402 769
Fragments arranged by size:
769 402 101
BstAPI GCAn_nnn'nTGC
Cuts at: 0 603 1272
Size: 603 669
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1272
Size: 831 441
BstYI r'GATC_y
Cuts at: 0 54 923 1272
Size: 54 869 349
Fragments arranged by size:
869 349 54
Bsu36I CC'TnA_GG
Cuts at: 0 928 1048 1272
Size: 928 120 224
Fragments arranged by size:
928 224 120
BtgI C'CryG_G
Cuts at: 0 1057 1272
Size: 1057 215
BtsI GCAGTG_nn'
Cuts at: 0 1036 1272
Size: 1036 236
DraIII CAC_nnn'GTG
Cuts at: 0 1213 1272
Size: 1213 59
EaeI y'GGCC_r
Cuts at: 0 204 312 521 725 980 1272
Size: 204 108 209 204 255 292
Fragments arranged by size:
292 255 209 204 204 108
EarI CTCTTCn'nnn_
Cuts at: 0 1086 1272
Size: 1086 186
EcoICRI GAG'CTC
Cuts at: 0 537 1239 1272
Size: 537 702 33
Fragments arranged by size:
702 537 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 309 1040 1272
Size: 63 246 731 232
Fragments arranged by size:
731 246 232 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 982 1272
Size: 982 290
EcoO109I rG'GnC_Cy
Cuts at: 0 21 303 1044 1272
Size: 21 282 741 228
Fragments arranged by size:
741 282 228 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 582 1272
Size: 582 690
HaeII r_GCGC'y
Cuts at: 0 847 1272
Size: 847 425
MluI A'CGCG_T
Cuts at: 0 1174 1272
Size: 1174 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 998 1272
Size: 404 594 274
Fragments arranged by size:
594 404 274
MscI TGG'CCA
Cuts at: 0 206 523 727 982 1272
Size: 206 317 204 255 290
Fragments arranged by size:
317 290 255 206 204
MslI CAynn'nnrTG
Cuts at: 0 121 379 511 589 1272
Size: 121 258 132 78 683
Fragments arranged by size:
683 258 132 121 78
MspA1I CmG'CkG
Cuts at: 0 51 471 1272
Size: 51 420 801
Fragments arranged by size:
801 420 51
NspI r_CATG'y
Cuts at: 0 631 840 1272
Size: 631 209 432
Fragments arranged by size:
631 432 209
PciI A'CATG_T
Cuts at: 0 836 1272
Size: 836 436
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 1272
Size: 474 798
PmlI CAC'GTG
Cuts at: 0 277 1272
Size: 277 995
PpuMI rG'GwC_Cy
Cuts at: 0 21 1272
Size: 21 1251
PstI C_TGCA'G
Cuts at: 0 599 1043 1272
Size: 599 444 229
Fragments arranged by size:
599 444 229
PvuII CAG'CTG
Cuts at: 0 51 471 1272
Size: 51 420 801
Fragments arranged by size:
801 420 51
SacI G_AGCT'C
Cuts at: 0 539 1241 1272
Size: 539 702 31
Fragments arranged by size:
702 539 31
SfcI C'TryA_G
Cuts at: 0 595 1039 1272
Size: 595 444 233
Fragments arranged by size:
595 444 233
SphI G_CATG'C
Cuts at: 0 631 1272
Size: 631 641
StyI C'CwwG_G
Cuts at: 0 38 557 983 1272
Size: 38 519 426 289
Fragments arranged by size:
519 426 289 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 51 1050 1272
Size: 51 999 222
Fragments arranged by size:
999 222 51
TatI w'GTAC_w
Cuts at: 0 101 254 503 785 938 1272
Size: 101 153 249 282 153 334
Fragments arranged by size:
334 282 249 153 153 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 1272
Size: 201 32 1039
Fragments arranged by size:
1039 201 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI AvaI BaeI BanI
BanII BbsI Bme1580I BmgBI BmrI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII MluI MmeI
MscI MslI MspA1I NspI PciI PflMI PmlI PpuMI
PstI PvuII SacI SfcI SphI StyI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI Bpu10I BpuEI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI