(Circular) (Six-base) MAPSORT of: pFN21AE0740.seq Check: 4757 from: 1 to: 5977 pFN21AE0740 5977 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2328 2482 2902 4040 5424 5692 1632 Size: 696 154 420 1138 1384 268 1917 Fragments arranged by size: 1917 1384 1138 696 420 268 154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2726 4876 5853 1203 Size: 1523 2150 977 1327 Fragments arranged by size: 2150 1523 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 3720 5292 3720 Size: 1572 4405 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2433 2532 3014 3229 3286 3512 3664 5773 Size: 940 99 482 215 57 226 152 2109 Cuts at: 5773 1493 Size: 1697 Fragments arranged by size: 2109 1697 940 482 226 215 152 99 57 AseI AT'TA_AT Cuts at: 160 1032 2187 4531 160 Size: 872 1155 2344 1606 Fragments arranged by size: 2344 1606 1155 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2027 3302 1870 Size: 73 84 1275 4545 Fragments arranged by size: 4545 1275 84 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2859 2892 2859 Size: 33 5944 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3296 1391 Size: 1905 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BbvCI CC'TCA_GC Cuts at: 2117 2117 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2577 5972 2577 Size: 3395 2582 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 4038 5194 1790 Size: 2248 1156 2573 Fragments arranged by size: 2573 2248 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2881 3708 4666 Size: 416 53 83 399 24 1464 827 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1464 958 827 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3185 1056 Size: 2129 3848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 Bpu10I CC'TnA_GC Cuts at: 2117 2117 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 4102 4967 5265 2061 Size: 2041 865 298 2773 Fragments arranged by size: 2773 2041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 3768 493 Size: 711 1503 1061 2702 Fragments arranged by size: 2702 1503 1061 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2024 3784 4166 Size: 53 83 186 936 476 15 1760 382 Cuts at: 4166 4801 275 Size: 635 1451 Fragments arranged by size: 1760 1451 936 635 476 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5067 BseYI C'CCAG_C Cuts at: 5180 5676 5180 Size: 496 5481 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2238 2674 1163 Size: 573 345 157 436 4466 Fragments arranged by size: 4466 573 436 345 157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3296 4038 4123 5194 1391 Size: 399 1506 742 85 1071 2174 Fragments arranged by size: 2174 1506 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2247 4472 4646 65 Size: 1183 999 2225 174 1396 Fragments arranged by size: 2225 1396 1183 999 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 2469 96 Size: 2373 3604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 3123 794 Size: 2329 3648 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2056 2577 3307 3674 4059 4076 5528 5614 Size: 84 521 730 367 385 17 1452 86 Cuts at: 5614 5626 5972 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 730 521 385 367 346 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2600 2862 2600 Size: 262 5715 BtgI C'CryG_G Cuts at: 513 1065 2004 3273 513 Size: 552 939 1269 3217 Fragments arranged by size: 3217 1269 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2997 487 Size: 683 56 1771 3467 Fragments arranged by size: 3467 1771 683 56 BtsI GCAGTG_nn' Cuts at: 2662 3493 4287 4307 2662 Size: 831 794 20 4332 Fragments arranged by size: 4332 831 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3196 3281 3196 Size: 85 5892 DraIII CAC_nnn'GTG Cuts at: 2904 2904 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942 Size: 54 1425 50 275 18 1594 889 1629 Cuts at: 5942 8 Size: 43 Fragments arranged by size: 1629 1594 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2906 2945 3164 3907 1353 Size: 1553 39 219 743 3423 Fragments arranged by size: 3423 1553 743 219 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2482 2902 4040 4637 5424 5692 Size: 393 696 154 420 1138 597 787 268 Cuts at: 5692 1239 Size: 1524 Fragments arranged by size: 1524 1138 787 696 597 420 393 268 154 EcoO109I rG'GnC_Cy Cuts at: 2112 2882 3105 2112 Size: 770 223 4984 Fragments arranged by size: 4984 770 223 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3156 3156 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 5124 1678 Size: 3446 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2569 2601 2951 2983 4618 Size: 32 135 32 997 32 350 32 1635 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1635 997 350 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2278 5091 5275 1305 Size: 973 2813 184 2007 Fragments arranged by size: 2813 2007 973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 2625 2835 3935 4294 4453 518 Size: 648 1393 66 210 1100 359 159 2042 Fragments arranged by size: 2042 1393 1100 648 359 210 159 66 MspA1I CmG'CkG Cuts at: 1606 3393 4070 5218 5463 1606 Size: 1787 677 1148 245 2120 Fragments arranged by size: 2120 1787 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 2735 387 Size: 2348 3629 NheI G'CTAG_C Cuts at: 1052 3181 1052 Size: 2129 3848 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2730 3126 3162 3181 3335 4880 1132 Size: 97 1501 396 36 19 154 1545 2229 Fragments arranged by size: 2229 1545 1501 396 154 97 36 19 PasI CC'CwG_GG Cuts at: 1348 2035 2448 1348 Size: 687 413 4877 Fragments arranged by size: 4877 687 413 PciI A'CATG_T Cuts at: 2726 4876 2726 Size: 2150 3827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2112 2112 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 2640 3547 2640 Size: 907 5070 PstI C_TGCA'G Cuts at: 830 2981 3329 830 Size: 2151 348 3478 Fragments arranged by size: 3478 2151 348 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2906 2945 3164 2906 Size: 39 219 5719 Fragments arranged by size: 5719 219 39 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2977 3325 4460 5141 5332 826 Size: 219 1932 348 1135 681 191 1471 Fragments arranged by size: 1932 1471 1135 681 348 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 4081 4982 5244 820 Size: 197 926 133 2005 901 262 1553 Fragments arranged by size: 2005 1553 926 901 262 197 133 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3162 3335 1132 Size: 2030 173 3774 Fragments arranged by size: 3774 2030 173 SspI AAT'ATT Cuts at: 5 52 2319 2408 3901 5 Size: 47 2267 89 1493 2081 Fragments arranged by size: 2267 2081 1493 89 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 3416 513 Size: 552 939 1412 3074 Fragments arranged by size: 3074 1412 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 4223 5935 Size: 274 80 33 51 1443 492 1754 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1754 1712 1443 492 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 5937 5969 2247 Size: 32 3658 32 2255 Fragments arranged by size: 3658 2255 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 2915 3218 4106 2915 Size: 303 888 4786 Fragments arranged by size: 4786 888 303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI