(Circular) (Six-base) MAPSORT of: pFN21AE0734.seq Check: 4879 from: 1 to: 5971 pFN21AE0734 5971 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2285 2285 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2524 3314 1937 Size: 587 790 4594 Fragments arranged by size: 4594 790 587 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 4034 5418 5686 1632 Size: 487 1915 1384 268 1917 Fragments arranged by size: 1917 1915 1384 487 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaLI G'TGCA_C Cuts at: 2070 2468 4028 5184 2070 Size: 398 1560 1156 2857 Fragments arranged by size: 2857 1560 1156 398 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3054 3296 1870 Size: 73 1111 242 4545 Fragments arranged by size: 4545 1111 242 73 AvrII C'CTAG_G Cuts at: 2713 2713 Size: 5971 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2178 2364 3163 3290 1391 Size: 787 186 799 127 4072 Fragments arranged by size: 4072 799 787 186 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 3257 928 Size: 971 527 831 3642 Fragments arranged by size: 3642 971 831 527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3083 3117 4162 4196 1903 Size: 34 1146 34 1045 34 3678 Fragments arranged by size: 3678 1146 1045 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3258 3388 3258 Size: 130 5841 Bme1580I G_kGCm'C Cuts at: 1790 2074 2472 4032 5188 1790 Size: 284 398 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 398 284 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4660 442 Size: 416 53 83 399 24 2285 958 1753 Fragments arranged by size: 2285 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2765 2765 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2994 4631 1239 Size: 1139 616 1637 2579 Fragments arranged by size: 2579 1637 1139 616 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 4096 4961 5259 2750 Size: 1346 865 298 3462 Fragments arranged by size: 3462 1346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 2989 4634 882 Size: 617 810 680 1645 2219 Fragments arranged by size: 2219 1645 810 680 617 BsaAI yAC'GTr Cuts at: 493 1204 2243 2467 3028 3762 493 Size: 711 1039 224 561 734 2702 Fragments arranged by size: 2702 1039 734 711 561 224 BsaBI GATnn'nnATC Cuts at: 2438 5965 2438 Size: 3527 2444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 1178 Size: 30 165 30 969 30 4747 Fragments arranged by size: 4747 969 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2870 1186 Size: 900 784 4287 Fragments arranged by size: 4287 900 784 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2313 1163 Size: 573 577 4821 Fragments arranged by size: 4821 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 2178 2248 2364 2472 4032 4117 5188 2074 Size: 104 70 116 108 1560 85 1071 2857 Fragments arranged by size: 2857 1560 1071 116 108 104 85 70 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2178 2248 2364 2472 3163 3290 Size: 399 284 104 70 116 108 691 127 Cuts at: 3290 4032 4117 5188 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 284 127 116 108 104 85 70 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 3326 844 Size: 1441 1041 3489 Fragments arranged by size: 3489 1441 1041 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 3103 4775 3103 Size: 1672 4299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5971 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2349 3153 513 Size: 552 939 51 294 804 3331 Fragments arranged by size: 3331 939 804 552 294 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2074 2296 3487 4281 4301 2074 Size: 222 1191 794 20 3744 Fragments arranged by size: 3744 1191 794 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 3080 3418 Size: 54 1425 50 275 18 744 506 338 Cuts at: 3418 4307 5936 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 744 506 338 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3105 3901 1353 Size: 1586 166 796 3423 Fragments arranged by size: 3423 1586 796 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2176 2362 2176 Size: 186 5785 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2378 2994 4034 4631 5418 5686 Size: 393 487 259 616 1040 597 787 268 Cuts at: 5686 1239 Size: 1524 Fragments arranged by size: 1524 1040 787 616 597 487 393 268 259 EcoO109I rG'GnC_Cy Cuts at: 2126 2126 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 EcoRV GAT'ATC Cuts at: 2792 2792 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 3265 5118 1678 Size: 1587 1853 2531 Fragments arranged by size: 2531 1853 1587 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3068 3100 4612 4644 4686 Size: 32 135 32 1496 32 1512 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 1512 1496 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2525 3315 3561 669 Size: 988 281 587 790 246 3079 Fragments arranged by size: 3079 988 790 587 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 10 Size: 54 1425 343 744 3405 Fragments arranged by size: 3405 1425 744 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3168 3929 4288 4447 518 Size: 648 2002 761 359 159 2042 Fragments arranged by size: 2042 2002 761 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2446 3387 4064 5212 5457 1606 Size: 840 941 677 1148 245 2120 Fragments arranged by size: 2120 1148 941 840 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2055 2349 513 Size: 552 939 51 294 4135 Fragments arranged by size: 4135 939 552 294 51 NdeI CA'TA_TG Cuts at: 387 2721 387 Size: 2334 3637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2549 3329 4874 1132 Size: 97 1320 780 1545 2229 Fragments arranged by size: 2229 1545 1320 780 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PmlI CAC'GTG Cuts at: 2243 2243 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2126 2126 Size: 5971 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 2277 3541 2277 Size: 1264 4707 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2471 2503 3006 3038 2471 Size: 32 503 32 5404 Fragments arranged by size: 5404 503 32 32 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2446 2446 Size: 5971 SacI G_AGCT'C Cuts at: 2178 2364 2178 Size: 186 5785 SalI G'TCGA_C Cuts at: 1936 2523 3313 1936 Size: 587 790 4594 Fragments arranged by size: 4594 790 587 SanDI GG'GwC_CC Cuts at: 2126 2126 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3319 4454 5135 5326 826 Size: 219 2274 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 3054 4075 4976 5238 820 Size: 197 926 822 289 1021 901 262 1553 Fragments arranged by size: 1553 1021 926 901 822 289 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 2349 2365 2577 2713 3410 Size: 552 939 51 294 16 212 136 697 Cuts at: 3410 513 Size: 3074 Fragments arranged by size: 3074 939 697 552 294 212 136 51 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2418 3998 4015 4168 4353 4798 325 Size: 1255 838 1580 17 153 185 445 1498 Fragments arranged by size: 1580 1498 1255 838 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 4217 5929 Size: 274 80 33 51 1443 288 1952 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1952 1712 1443 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3203 3235 5931 5963 3203 Size: 32 2696 32 3211 Fragments arranged by size: 3211 2696 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 3054 1943 Size: 1111 4860 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI