Restriction map for
pFN21AE0733
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tru9I Tsp509I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 30 CGCG CG^CG
ApaI 396 GGGCCC GGGCC^C
AspHI 742 GAGCTC G[AT]GC[AT]^C
AspI 226 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 136 GTCTTC GAAGAC(2/6)
BcgI 131 GCACCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 907 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 739 GCTGAGC GC^T[ACGT]AGC
BpmI 166 CTCCAG CTGGAG(16/14)
Bpu1102I 739 GCTGAGC GC^T[ACGT]AGC
BpuAI 136 GTCTTC GAAGAC(2/6)
BseRI 679 CTCCTC GAGGAG(10/8)
BsgI 272 GTGCAG GTGCAG(16/14)
BsiHKAI 742 GAGCTC G[AT]GC[AT]^C
Bsp1407I 605 TGTACA T^GTACA
BspHI 567 TCATGA T^CATGA
BspMI 274 GCAGGT ACCTGC(4/8)
BsrBI 349 GAGCGG CCGCTC(-3/-3)
BsrFI 791 GCCGGT [GA]^CCGG[CT]
BsrGI 605 TGTACA T^GTACA
BstUI 30 CGCG CG^CG
BstXI 462 CCATTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 739 GCTGAGC GC^T[ACGT]AGC
CfoI 31 GCGC GCG^C
Cfr10I 791 GCCGGT [GA]^CCGG[CT]
DraIII 401 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 832 GAAGAG CTCTTC(1/4)
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
HincII 3 GTTGAC GT[CT]^[GA]AC
HindII 3 GTTGAC GT[CT]^[GA]AC
HphI 406 GGTGA GGTGA(8/7)
Ksp632I 832 GAAGAG CTCTTC(1/4)
MspA1I 420 CCGCTG C[AC]G^C[GT]G
MvnI 30 CGCG CG^CG
NspI 235 ACATGC [GA]CATG^[CT]
PaeR7I 346 CTCGAG C^TCGAG
PflFI 226 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 567 TCATGA T^CATGA
SacI 742 GAGCTC GAGCT^C
SfcI 16 CTACAG C^T[GA][CT]AG
SgrAI 790 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 37 CCCGGG CCC^GGG
SmlI 346 CTCGAG C^T[CT][GA]AG
SspBI 605 TGTACA T^GTACA
Tth111I 226 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 346 CTCGAG C^TCGAG
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 738 AGCT AG^CT
743 AGCT
AvaI 37 CCCGGG C^[CT]CG[GA]G
346 CTCGAG
BalI 173 TGGCCA TGG^CCA
432 TGGCCA
BanII 396 GGGCCC G[GA]GC[CT]^C
742 GAGCTC
BbvI 445 GCAGC GCAGC(8/12)
422 GCTGC
BsmAI 319 GAGAC GTCTC(1/5)
763 GTCTC
BsoBI 37 CCCGGG C^[CT]CG[GA]G
346 CTCGAG
Cac8I 302 GCCTGC GC[ACGT]^[ACGT]GC
360 GCCCGC
DdeI 228 CTCAG C^T[ACGT]AG
740 CTGAG
DsaI 468 CCGTGG C^C[GA][CT]GG
522 CCGTGG
EcoNI 111 CCTGGGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
710 CCTTTGAGAGG
FauI 28 CCCGC CCCGC(4/6)
361 CCCGC
MluNI 173 TGGCCA TGG^CCA
432 TGGCCA
MscI 173 TGGCCA TGG^CCA
432 TGGCCA
PflMI 435 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
656 CCACTGTCTGG
SfaNI 855 GATGC GCATC(5/9)
674 GCATC
TseI 422 GCTGC G^C[AT]GC
445 GCAGC
Van91I 435 CCAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
656 CCACTGTCTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AlwI AvaII BanI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TaqI TfiI
Tsp45I TspRI TthHB8I XhoII