Restriction map for
pFN21AE0733
(Linear) (Six-base) MAPSORT of: KIEE0733.seq Check: 2284 from: 1 to: 933
KIEE0733.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 265 933
Size: 265 668
ApaI G_GGCC'C
Cuts at: 0 400 933
Size: 400 533
AvaI C'yCGr_G
Cuts at: 0 37 346 933
Size: 37 309 587
Fragments arranged by size:
587 309 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 622 655 933
Size: 622 33 278
Fragments arranged by size:
622 278 33
BanI G'GyrC_C
Cuts at: 0 130 284 556 794 933
Size: 130 154 272 238 139
Fragments arranged by size:
272 238 154 139 130
BanII G_rGCy'C
Cuts at: 0 400 746 933
Size: 400 346 187
Fragments arranged by size:
400 346 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 933
Size: 129 804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 154 933
Size: 120 34 779
Fragments arranged by size:
779 120 34
BglI GCCn_nnn'nGGC
Cuts at: 0 913 933
Size: 913 20
BlpI GC'TnA_GC
Cuts at: 0 740 933
Size: 740 193
Bme1580I G_kGCm'C
Cuts at: 0 287 400 561 783 933
Size: 287 113 161 222 150
Fragments arranged by size:
287 222 161 150 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 412 679 933
Size: 412 267 254
Fragments arranged by size:
412 267 254
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 642 933
Size: 642 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 933
Size: 151 782
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 933
Size: 670 263
BseYI C'CCAG_C
Cuts at: 0 204 753 933
Size: 204 549 180
Fragments arranged by size:
549 204 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 933
Size: 293 640
BsiHKAI G_wGCw'C
Cuts at: 0 746 933
Size: 746 187
Bsp1286I G_dGCh'C
Cuts at: 0 287 400 561 746 783 933
Size: 287 113 161 185 37 150
Fragments arranged by size:
287 185 161 150 113 37
BspHI T'CATG_A
Cuts at: 0 567 933
Size: 567 366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 265 933
Size: 265 668
BsrBI CCG'CTC
Cuts at: 0 351 933
Size: 351 582
BsrFI r'CCGG_y
Cuts at: 0 791 933
Size: 791 142
BsrGI T'GTAC_A
Cuts at: 0 605 933
Size: 605 328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 469 933
Size: 469 464
BstYI r'GATC_y
Cuts at: 0 369 623 836 933
Size: 369 254 213 97
Fragments arranged by size:
369 254 213 97
BtgI C'CryG_G
Cuts at: 0 468 522 933
Size: 468 54 411
Fragments arranged by size:
468 411 54
BtsI GCAGTG_nn'
Cuts at: 0 253 456 933
Size: 253 203 477
Fragments arranged by size:
477 253 203
DraIII CAC_nnn'GTG
Cuts at: 0 406 933
Size: 406 527
EaeI y'GGCC_r
Cuts at: 0 173 432 653 933
Size: 173 259 221 280
Fragments arranged by size:
280 259 221 173
EarI CTCTTCn'nnn_
Cuts at: 0 827 933
Size: 827 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 289 778 933
Size: 289 489 155
Fragments arranged by size:
489 289 155
EcoICRI GAG'CTC
Cuts at: 0 744 933
Size: 744 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 933
Size: 151 782
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 714 933
Size: 115 599 219
Fragments arranged by size:
599 219 115
EcoO109I rG'GnC_Cy
Cuts at: 0 22 41 396 901 933
Size: 22 19 355 505 32
Fragments arranged by size:
505 355 32 22 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 933
Size: 141 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 137 169 933
Size: 29 108 32 764
Fragments arranged by size:
764 108 32 29
HincII GTy'rAC
Cuts at: 0 5 933
Size: 5 928
MscI TGG'CCA
Cuts at: 0 175 434 933
Size: 175 259 499
Fragments arranged by size:
499 259 175
MslI CAynn'nnrTG
Cuts at: 0 181 258 467 933
Size: 181 77 209 466
Fragments arranged by size:
466 209 181 77
MspA1I CmG'CkG
Cuts at: 0 422 933
Size: 422 511
NspI r_CATG'y
Cuts at: 0 239 933
Size: 239 694
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 662 933
Size: 441 221 271
Fragments arranged by size:
441 271 221
PpuMI rG'GwC_Cy
Cuts at: 0 41 933
Size: 41 892
PspOMI G'GGCC_C
Cuts at: 0 396 933
Size: 396 537
PspXI vC'TCGA_Gb
Cuts at: 0 346 933
Size: 346 587
SacI G_AGCT'C
Cuts at: 0 746 933
Size: 746 187
SanDI GG'GwC_CC
Cuts at: 0 41 933
Size: 41 892
SfcI C'TryA_G
Cuts at: 0 16 933
Size: 16 917
SgrAI Cr'CCGG_yG
Cuts at: 0 791 933
Size: 791 142
SmaI CCC'GGG
Cuts at: 0 39 933
Size: 39 894
SmlI C'TyrA_G
Cuts at: 0 346 933
Size: 346 587
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 259 394 933
Size: 259 135 539
Fragments arranged by size:
539 259 135
TatI w'GTAC_w
Cuts at: 0 605 933
Size: 605 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 448 480 659 691 933
Size: 255 32 161 32 179 32 242
Fragments arranged by size:
255 242 179 161 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 229 933
Size: 229 704
XhoI C'TCGA_G
Cuts at: 0 346 933
Size: 346 587
XmaI C'CCGG_G
Cuts at: 0 37 933
Size: 37 896
Enzymes that do cut:
AarI ApaI AvaI BaeI BanI BanII BbsI BcgI
BglI BlpI Bme1580I BmrI BplI BpmI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MscI
MslI MspA1I NspI PflMI PpuMI PspOMI PspXI SacI
SanDI SfcI SgrAI SmaI SmlI TaqII TatI TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI XbaI XcmI XmnI
ZraI