Restriction map for
pFN21AE0729
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 157 AAATTT [GA]^AATT[CT]
Alw44I 565 GTGCAC G^TGCAC
AlwNI 856 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 579 CCTGAGG CC^T[ACGT]AGG
ApaI 794 GGGCCC GGGCC^C
ApaLI 565 GTGCAC G^TGCAC
ApoI 157 AAATTT [GA]^AATT[CT]
AvaI 797 CCCGGG C^[CT]CG[GA]G
BanI 107 GGCGCC G^G[CT][GA]CC
BbeI 107 GGCGCC GGCGC^C
BbsI 588 GAAGAC GAAGAC(2/6)
BpmI 250 CTGGAG CTGGAG(16/14)
BpuAI 588 GAAGAC GAAGAC(2/6)
BsgI 22 GTGCAG GTGCAG(16/14)
BsoBI 797 CCCGGG C^[CT]CG[GA]G
BstAPI 255 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 750 CATCC GGATG(2/0)
BstXI 11 CCAGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 579 CCTGAGG CC^T[ACGT]AGG
DraII 793 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco81I 579 CCTGAGG CC^T[ACGT]AGG
EcoO109I 793 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 704 GATATC GAT^ATC
FokI 750 CATCC GGATG(9/13)
KasI 107 GGCGCC G^GCGCC
MseI 500 TTAA T^TAA
MslI 672 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 107 GGCGCC GG^CGCC
NdeI 644 CATATG CA^TATG
PflMI 828 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 43 CCTGCAGG CCTGCA^GG
SfoI 107 GGCGCC GGC^GCC
SmaI 797 CCCGGG CCC^GGG
Sse8387I 43 CCTGCAGG CCTGCA^GG
TaqI 119 TCGA T^CGA
Tru9I 500 TTAA T^TAA
TthHB8I 119 TCGA T^CGA
Van91I 828 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 797 CCCGGG C^CCGGG
XmaI 797 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 619 CCGC CCGC(-3/-1)
105 GCGG
AcyI 107 GGCGCC G[GA]^CG[CT]C
137 GGCGTC
AfaI 171 GTAC GT^AC
348 GTAC
AlwI 279 GATCC GGATC(4/5)
858 GATCC
BsaHI 107 GGCGCC G[GA]^CG[CT]C
137 GGCGTC
BseRI 442 GAGGAG GAGGAG(10/8)
582 GAGGAG
BsmAI 736 GAGAC GTCTC(1/5)
840 GAGAC
BspMI 42 ACCTGC ACCTGC(4/8)
512 ACCTGC
BsrI 249 ACTGG ACTGG(1/-1)
606 ACTGG
BstYI 278 AGATCC [GA]^GATC[CT]
857 AGATCC
Cac8I 46 GCAGGC GC[ACGT]^[ACGT]GC
337 GCACGC
DpnII 279 GATC ^GATC
858 GATC
EarI 220 CTCTTC CTCTTC(1/4)
52 GAAGAG
EcoNI 144 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
575 CCTCCCTGAGG
EcoT14I 370 CCAAGG C^C[AT][AT]GG
833 CCTTGG
HaeII 107 GGCGCC [GA]GCGC^[CT]
721 AGCGCC
HgaI 746 GACGC GACGC(5/10)
138 GCGTC
Hin1I 107 GGCGCC G[GA]^CG[CT]C
137 GGCGTC
HinfI 354 GATTC G^A[ACGT]TC
486 GATTC
Ksp632I 220 CTCTTC CTCTTC(1/4)
52 GAAGAG
MboI 279 GATC ^GATC
858 GATC
MflI 278 AGATCC [GA]^GATC[CT]
857 AGATCC
MspA1I 25 CAGCTG C[AC]G^C[GT]G
259 CAGCTG
NdeII 279 GATC ^GATC
858 GATC
PstI 44 CTGCAG CTGCA^G
394 CTGCAG
PvuII 25 CAGCTG CAG^CTG
259 CAGCTG
RsaI 171 GTAC GT^AC
348 GTAC
SacI 3 GAGCTC GAGCT^C
379 GAGCTC
Sau3AI 279 GATC ^GATC
858 GATC
SmlI 184 CTCAAG C^T[CT][GA]AG
754 CTCAAG
StyI 370 CCAAGG C^C[AT][AT]GG
833 CCTTGG
TfiI 354 GATTC G^A[AT]TC
486 GATTC
TspRI 605 CACTG CA[GC]TG[ACGT][ACGT]^
732 CAGTG
XhoII 278 AGATCC [GA]^GATC[CT]
857 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AspHI BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI CfoI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI SfcI TseI Tsp509I