Restriction map for
pFN21AE0729
(Circular) (Six-base) MAPSORT of: pFN21AE0729.seq Check: 9480 from: 1 to: 5632
pFN21AE0729 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2888 3695 5079 5347 1632
Size: 605 651 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 651 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2699 1184
Size: 1515 4117
AlwNI CAG_nnn'CTG
Cuts at: 2866 3375 4947 2866
Size: 509 1572 3551
Fragments arranged by size:
3551 1572 509
ApaI G_GGCC'C
Cuts at: 2803 2803
Size: 5632
ApaLI G'TGCA_C
Cuts at: 2570 3689 4845 2570
Size: 1119 1156 3357
Fragments arranged by size:
3357 1156 1119
ApoI r'AATT_y
Cuts at: 1493 2162 2941 3167 3319 5428 1493
Size: 669 779 226 152 2109 1697
Fragments arranged by size:
2109 1697 779 669 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2802 2957 1870
Size: 73 859 155 4545
Fragments arranged by size:
4545 859 155 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2953 4408 618
Size: 325 217 627 325 841 1455 1842
Fragments arranged by size:
1842 1455 841 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2388 2803 2951 1391
Size: 621 376 415 148 4072
Fragments arranged by size:
4072 621 415 376 148
BbeI G_GCGC'C
Cuts at: 2116 2116
Size: 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2600 928
Size: 971 701 3960
Fragments arranged by size:
3960 971 701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2574 2803 3693 4849 1790
Size: 784 229 890 1156 2573
Fragments arranged by size:
2573 1156 890 784 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 4292 1239
Size: 1037 2016 2579
Fragments arranged by size:
2579 2016 1037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2744 3757 4622 4920 2174
Size: 570 1013 865 298 2886
Fragments arranged by size:
2886 1013 865 570 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2143 3439 3821
Size: 53 83 186 936 580 30 1296 382
Cuts at: 3821 4456 275
Size: 635 1451
Fragments arranged by size:
1451 1296 936 635 580 382 186 83
53 30
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2564 2594 1178
Size: 30 165 30 1161 30 4216
Fragments arranged by size:
4216 1161 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 2602 1186
Size: 1276 140 4216
Fragments arranged by size:
4216 1276 140
BseYI C'CCAG_C
Cuts at: 2032 2085 2093 2101 2524 4835 5331 2032
Size: 53 8 8 423 2311 496 2333
Fragments arranged by size:
2333 2311 496 423 53 8 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 1163
Size: 573 312 4747
Fragments arranged by size:
4747 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2262 2388 2574 2688 2920 3693 3778 4849
Size: 250 126 186 114 232 773 85 1071
Cuts at: 4849 2012
Size: 2795
Fragments arranged by size:
2795 1071 773 250 232 186 126 114
85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2262 2388 2574 2688 2803 2920
Size: 399 222 250 126 186 114 115 117
Cuts at: 2920 2951 3693 3778 4849 1391
Size: 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 250 222 186 126
117 115 114 85 31
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 2526 2987 844
Size: 1212 470 461 3489
Fragments arranged by size:
3489 1212 470 461
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 4160
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2023
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2283 2862 2962 3329 3714 3731 5183 5269
Size: 311 579 100 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 579 385 367 346 311 100
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2585 2585
Size: 5632
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5080
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597
Size: 54 1425 50 275 18 1249 889 1629
Cuts at: 5597 8
Size: 43
Fragments arranged by size:
1629 1425 1249 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2231 3562 1353
Size: 699 179 1331 3423
Fragments arranged by size:
3423 1331 699 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2386 2010
Size: 376 5256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2276 2888 3695 4292 5079 5347
Size: 393 605 39 612 807 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 807 787 612 605 597 393 268
39
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2584 2153
Size: 431 5201
EcoO109I rG'GnC_Cy
Cuts at: 2799 2799
Size: 5632
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
EcoRV GAT'ATC
Cuts at: 2711 2711
Size: 5632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2116 2730 4779 1678
Size: 438 614 2049 2531
Fragments arranged by size:
2531 2049 614 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2275 2307 2742 2774 4273
Size: 32 135 32 703 32 435 32 1499
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1499 703 435 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2112 2112
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 3590 3949 4108 518
Size: 648 1515 909 359 159 2042
Fragments arranged by size:
2042 1515 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2266 3048 3725 4873 5118 1606
Size: 426 234 782 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 677 426 245 234
NarI GG'CG_CC
Cuts at: 2113 2113
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 2650 387
Size: 2263 3369
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2149 2877 1348
Size: 801 728 4103
Fragments arranged by size:
4103 801 728
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2839 1108
Size: 1731 3901
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2799 2799
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2053 2403 2984 830
Size: 1223 350 581 3478
Fragments arranged by size:
3478 1223 581 350
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2032 2266 2032
Size: 234 5398
SacI G_AGCT'C
Cuts at: 2012 2388 2012
Size: 376 5256
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2053 2984 2053
Size: 931 4701
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2049 2067 2399 2980 4115 4796 4987
Size: 219 1004 18 332 581 1135 681 191
Cuts at: 4987 826
Size: 1471
Fragments arranged by size:
1471 1135 1004 681 581 332 219 191
18
SfoI GGC'GCC
Cuts at: 2114 2114
Size: 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2804 2959 1872
Size: 932 155 4545
Fragments arranged by size:
4545 932 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2759 3736 4637 4899 820
Size: 197 926 246 570 977 901 262 1553
Fragments arranged by size:
1553 977 926 901 570 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2375 2838 3071 513
Size: 552 1310 463 233 3074
Fragments arranged by size:
3074 1310 552 463 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 2657 2689 5592 5624 2564
Size: 32 61 32 2903 32 2572
Fragments arranged by size:
2903 2572 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2802 2957 1870
Size: 932 155 4545
Fragments arranged by size:
4545 932 155
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI