(Circular) (Six-base) MAPSORT of: pFN21AE0726.seq Check: 1218 from: 1 to: 5956 pFN21AE0726 5956 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2338 2338 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2813 3299 1937 Size: 876 486 4594 Fragments arranged by size: 4594 876 486 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 4019 5403 5671 1632 Size: 600 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 600 268 AflII C'TTAA_G Cuts at: 820 1017 3224 820 Size: 197 2207 3552 Fragments arranged by size: 3552 2207 197 AflIII A'CryG_T Cuts at: 1203 2459 3179 4855 5832 1203 Size: 1256 720 1676 977 1327 Fragments arranged by size: 1676 1327 1256 977 720 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 2610 4685 2610 Size: 2075 3881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4705 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2116 2872 2976 3265 3491 3643 5752 1493 Size: 623 756 104 289 226 152 2109 1697 Fragments arranged by size: 2109 1697 756 623 289 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 2705 3286 3653 2705 Size: 581 367 5008 Fragments arranged by size: 5008 581 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2664 3277 4732 618 Size: 325 217 627 877 613 1455 1842 Fragments arranged by size: 1842 1455 877 627 613 325 217 BanII G_rGCy'C Cuts at: 1391 3275 1391 Size: 1884 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 2535 2706 928 Size: 971 375 261 171 4178 Fragments arranged by size: 4178 971 375 261 171 BbvCI CC'TCA_GC Cuts at: 2178 2178 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2462 3848 5058 2462 Size: 1386 1210 3360 Fragments arranged by size: 3360 1386 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2719 4017 5173 1790 Size: 929 1298 1156 2573 Fragments arranged by size: 2573 1298 1156 929 BmgBI CAC'GTC Cuts at: 1318 2035 2104 1318 Size: 717 69 5170 Fragments arranged by size: 5170 717 69 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2856 3687 4645 Size: 416 53 83 399 24 1439 831 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1439 958 831 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2246 2788 2246 Size: 542 5414 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 2758 2922 3006 3174 4616 1239 Size: 1054 465 164 84 168 1442 2579 Fragments arranged by size: 2579 1442 1054 465 168 164 84 Bpu10I CC'TnA_GC Cuts at: 2178 2178 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2452 4081 4946 5244 2452 Size: 1629 865 298 3164 Fragments arranged by size: 3164 1629 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2462 2636 3747 493 Size: 711 1258 174 1111 2702 Fragments arranged by size: 2702 1258 1111 711 174 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2876 4389 4787 5061 5208 5619 2876 Size: 1513 398 274 147 411 3213 Fragments arranged by size: 3213 1513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5070 BseYI C'CCAG_C Cuts at: 2077 2247 5159 5655 2077 Size: 170 2912 496 2378 Fragments arranged by size: 2912 2378 496 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3092 1163 Size: 573 1356 4027 Fragments arranged by size: 4027 1356 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4017 4102 5173 4017 Size: 85 1071 4800 Fragments arranged by size: 4800 1071 85 BsmI GAATG_Cn' Cuts at: 2132 3467 3560 2132 Size: 1335 93 4528 Fragments arranged by size: 4528 1335 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2094 2094 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2719 3275 4017 4102 5173 1391 Size: 399 929 556 742 85 1071 2174 Fragments arranged by size: 2174 1071 929 742 556 399 85 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 2380 3845 5575 2380 Size: 1465 1730 2761 Fragments arranged by size: 2761 1730 1465 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2338 2417 3311 844 Size: 1494 79 894 3489 Fragments arranged by size: 3489 1494 894 79 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2893 4451 4625 65 Size: 1183 1645 1558 174 1396 Fragments arranged by size: 1645 1558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2233 4016 5028 2233 Size: 1783 1012 3161 Fragments arranged by size: 3161 1783 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2858 3070 794 Size: 2064 212 3680 Fragments arranged by size: 3680 2064 212 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 2020 1184 Size: 836 5120 BstYI r'GATC_y Cuts at: 1972 2705 3286 3653 4038 4055 5507 5593 5605 Size: 733 581 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 733 581 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2814 2814 Size: 5956 Bsu36I CC'TnA_GG Cuts at: 2711 2711 Size: 5956 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5404 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4963 809 Size: 507 1221 2426 1802 Fragments arranged by size: 2426 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921 Size: 54 1425 50 275 18 1573 889 1629 Cuts at: 5921 8 Size: 43 Fragments arranged by size: 1629 1573 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3886 1353 Size: 2533 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2293 2758 2922 3006 3174 4019 Size: 393 600 61 465 164 84 168 845 Cuts at: 4019 4616 5403 5671 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 845 787 600 597 465 393 268 168 164 84 61 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2436 2468 2576 2596 2608 Size: 32 135 32 864 32 108 20 12 Cuts at: 2608 2628 4597 4629 4671 4703 1373 Size: 20 1969 32 42 32 2626 Fragments arranged by size: 2626 1969 864 135 108 42 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 2688 748 Size: 1940 4016 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2634 2801 3237 3914 4273 4432 518 Size: 648 1468 167 436 677 359 159 2042 Fragments arranged by size: 2042 1468 677 648 436 359 167 159 MspA1I CmG'CkG Cuts at: 1606 2671 3372 4049 5197 5442 1606 Size: 1065 701 677 1148 245 2120 Fragments arranged by size: 2120 1148 1065 701 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5404 NdeI CA'TA_TG Cuts at: 387 2970 387 Size: 2583 3373 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3068 3183 3314 4859 1132 Size: 97 1839 115 131 1545 2229 Fragments arranged by size: 2229 1839 1545 131 115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 3179 4855 3179 Size: 1676 4280 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PmlI CAC'GTG Cuts at: 2462 2462 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2227 2259 4025 4057 5538 5570 2227 Size: 32 1766 32 1481 32 2613 Fragments arranged by size: 2613 1766 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 2335 2414 3308 830 Size: 1505 79 894 3478 Fragments arranged by size: 3478 1505 894 79 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 2414 3308 2414 Size: 894 5062 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2095 2331 2410 3304 4439 5120 5311 Size: 219 1050 236 79 894 1135 681 191 Cuts at: 5311 826 Size: 1471 Fragments arranged by size: 1471 1135 1050 894 681 236 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2467 3224 4060 4961 5223 820 Size: 197 926 524 757 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 757 524 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 3395 513 Size: 552 2330 3074 Fragments arranged by size: 3074 2330 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI