Restriction map for
pFN21AE4142
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BsaAI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MslI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 940 AACGTT AA^CGTT
AcyI 310 GGCGTC G[GA]^CG[CT]C
AlwI 1432 GATCC GGATC(4/5)
AlwNI 742 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 299 AGCGCT AGC^GCT
AviII 1524 TGCGCA TGC^GCA
BalI 698 TGGCCA TGG^CCA
BcgI 996 GCAGGGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 520 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 520 GCTGAGC GC^T[ACGT]AGC
BsaBI 75 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 310 GGCGTC G[GA]^CG[CT]C
BsaI 562 GAGACC GGTCTC(1/5)
BsiEI 1469 CGGCCG CG[GA][CT]^CG
BspHI 1016 TCATGA T^CATGA
BsrBI 1504 GAGCGG CCGCTC(-3/-3)
BstYI 1431 AGATCC [GA]^GATC[CT]
CelII 520 GCTGAGC GC^T[ACGT]AGC
DpnII 1432 GATC ^GATC
EagI 1469 CGGCCG C^GGCCG
EclXI 1469 CGGCCG C^GGCCG
Eco47III 299 AGCGCT AGC^GCT
Eco52I 1469 CGGCCG C^GGCCG
EcoT22I 446 ATGCAT ATGCA^T
FspI 1524 TGCGCA TGC^GCA
Hin1I 310 GGCGTC G[GA]^CG[CT]C
KspI 482 CCGCGG CCGC^GG
MamI 75 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1432 GATC ^GATC
MflI 1431 AGATCC [GA]^GATC[CT]
MluNI 698 TGGCCA TGG^CCA
MscI 698 TGGCCA TGG^CCA
NaeI 12 GCCGGC GCC^GGC
NcoI 989 CCATGG C^CATGG
NdeII 1432 GATC ^GATC
NgoMIV 12 GCCGGC G^CCGGC
NsiI 446 ATGCAT ATGCA^T
NspI 143 GCATGT [GA]CATG^[CT]
Psp1406I 940 AACGTT AA^CGTT
PstI 1048 CTGCAG CTGCA^G
RcaI 1016 TCATGA T^CATGA
SacII 482 CCGCGG CCGC^GG
SapI 24 GAAGAGC GCTCTTC(1/4)
Sau3AI 1432 GATC ^GATC
SbfI 1047 CCTGCAGG CCTGCA^GG
SfcI 1048 CTGCAG C^T[GA][CT]AG
SmlI 688 CTCAAG C^T[CT][GA]AG
Sse8387I 1047 CCTGCAGG CCTGCA^GG
TaqI 338 TCGA T^CGA
Tsp45I 1697 GTGAC ^GT[GC]AC
TthHB8I 338 TCGA T^CGA
XhoII 1431 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 132 GTAC GT^AC
279 GTAC
AspI 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACGCAGTC
BanI 581 GGTGCC G^G[CT][GA]CC
1597 GGTGCC
BbsI 868 GAAGAC GAAGAC(2/6)
1129 GTCTTC
BfaI 442 CTAG C^TAG
1121 CTAG
BpuAI 868 GAAGAC GAAGAC(2/6)
1129 GTCTTC
BsmAI 562 GAGAC GTCTC(1/5)
1668 GTCTC
BsmI 774 GAATGC GAATGC(1/-1)
1001 GCATTC
EarI 24 GAAGAG CTCTTC(1/4)
45 GAAGAG
EcoNI 93 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1629 CCTCCAGGAGG
HinfI 208 GACTC G^A[ACGT]TC
1401 GACTC
Ksp632I 24 GAAGAG CTCTTC(1/4)
45 GAAGAG
MaeI 442 CTAG C^TAG
1121 CTAG
MaeIII 938 GTAAC ^GT[ACGT]AC
1697 GTGAC
PflFI 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACGCAGTC
PleI 208 GACTC GAGTC(4/5)
1401 GACTC
RsaI 132 GTAC GT^AC
279 GTAC
SmaI 902 CCCGGG CCC^GGG
1284 CCCGGG
Tth111I 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACGCAGTC
XmaCI 902 CCCGGG C^CCGGG
1284 CCCGGG
XmaI 902 CCCGGG C^CCGGG
1284 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AccIII AciI AluI ApaI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MboII MnlI MroI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PvuII
Sau96I ScrFI SfaNI StyI Tru9I
TseI TspRI XcmI