Restriction map for
pFN21AE4142
(Circular) (Six-base) MAPSORT of: pFN21AE4142.seq Check: 126 from: 1 to: 6409
pFN21AE4142 6409 bp
With 182 enzymes: SgfI *
March 28, 2012 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2760 3574 3689 2760
Size: 814 115 5480
Fragments arranged by size:
5480 814 115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3752 1937
Size: 1815 4594
Acc65I G'GTAC_C
Cuts at: 3730 3730
Size: 6409
AclI AA'CG_TT
Cuts at: 1563 2946 3708 4536 4909 1563
Size: 1383 762 828 373 3063
Fragments arranged by size:
3063 1383 828 762 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2836 3121 3508 4472 5856 6124 1632
Size: 458 746 285 387 964 1384 268 1917
Fragments arranged by size:
1917 1384 964 746 458 387 285 268
AfeI AGC'GCT
Cuts at: 2306 2306
Size: 6409
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 5308 6285 1203
Size: 4105 977 1327
Fragments arranged by size:
4105 1327 977
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2517 1184
Size: 1333 5076
AlwNI CAG_nnn'CTG
Cuts at: 2752 4152 5724 2752
Size: 1400 1572 3437
Fragments arranged by size:
3437 1572 1400
ApaI G_GGCC'C
Cuts at: 2176 3062 3647 2176
Size: 886 585 4938
Fragments arranged by size:
4938 886 585
ApaLI G'TGCA_C
Cuts at: 4466 5622 4466
Size: 1156 5253
ApoI r'AATT_y
Cuts at: 1493 3718 3944 4096 6205 1493
Size: 2225 226 152 2109 1697
Fragments arranged by size:
2225 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3289 3608 3734 1870
Size: 73 964 382 319 126 4545
Fragments arranged by size:
4545 964 382 319 126 73
BamHI G'GATC_C
Cuts at: 3739 4106 3739
Size: 367 6042
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2586 3602 3730 5185 618
Size: 325 217 627 799 1016 128 1455 1842
Fragments arranged by size:
1842 1455 1016 799 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2176 2388 2684 2914 3062 3647 3728 1391
Size: 785 212 296 230 148 585 81 4072
Fragments arranged by size:
4072 785 585 296 230 212 148 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2880 3127 928
Size: 971 981 247 4210
Fragments arranged by size:
4210 981 971 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2990 3024 4600 4634 1903
Size: 34 1053 34 1576 34 3678
Fragments arranged by size:
3678 1576 1053 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4301 5511 4301
Size: 1210 5199
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BfrBI ATG'CAT
Cuts at: 2453 2453
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 2768 2975
Size: 107 122 71 631 605 508 588 207
Cuts at: 2975 3611 3651 5020 136
Size: 636 40 1369 1525
Fragments arranged by size:
1525 1369 636 631 605 588 508 207
122 107 71 40
BglII A'GATC_T
Cuts at: 6404 6404
Size: 6409
BlpI GC'TnA_GC
Cuts at: 2526 3826 2526
Size: 1300 5109
Bme1580I G_kGCm'C
Cuts at: 1790 2176 2835 3062 3607 3647 4470 5626 1790
Size: 386 659 227 545 40 823 1156 2573
Fragments arranged by size:
2573 1156 823 659 545 386 227 40
BmgBI CAC'GTC
Cuts at: 1318 3133 3372 1318
Size: 1815 239 4355
Fragments arranged by size:
4355 1815 239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3218 4140 5098
Size: 416 53 83 399 24 1801 922 958
Cuts at: 5098 442
Size: 1753
Fragments arranged by size:
1801 1753 958 922 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2249 2282 3169 3281 3620 5069 1239
Size: 881 129 33 887 112 339 1449 2579
Fragments arranged by size:
2579 1449 887 881 339 129 112 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 4534 5399 5697 2678
Size: 1856 865 298 3390
Fragments arranged by size:
3390 1856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 5072 882
Size: 617 1062 2511 2219
Fragments arranged by size:
2511 2219 1062 617
BsaAI yAC'GTr
Cuts at: 493 1204 4200 493
Size: 711 2996 2702
Fragments arranged by size:
2996 2702 711
BsaBI GATnn'nnATC
Cuts at: 2084 6403 2084
Size: 4319 2090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 4216 4598 5233
Size: 53 83 186 936 783 1900 382 635
Cuts at: 5233 275
Size: 1451
Fragments arranged by size:
1900 1451 936 783 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2193 2399 2605 3043 3570 4842 5240 5514 5661
Size: 206 206 438 527 1272 398 274 147
Cuts at: 5661 6072 2193
Size: 411 2530
Fragments arranged by size:
2530 1272 527 438 411 398 274 206
206 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3200 3230 3583 3613 1178
Size: 30 165 30 1797 30 353 30 3974
Fragments arranged by size:
3974 1797 353 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3178 3181 3212 3215 3271 3447 3467 3604
Size: 1992 3 31 3 56 176 20 137
Cuts at: 3604 1186
Size: 3991
Fragments arranged by size:
3991 1992 176 137 56 31 20 3
3
BseYI C'CCAG_C
Cuts at: 3321 5612 6108 3321
Size: 2291 496 3622
Fragments arranged by size:
3622 2291 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 2231 2737 3008 1163
Size: 573 338 157 506 271 4564
Fragments arranged by size:
4564 573 506 338 271 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3477 3859 4620 5648 1540
Size: 275 186 1476 382 761 1028 2301
Fragments arranged by size:
2301 1476 1028 761 382 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2532 2850 4470 4555 5626 2268
Size: 264 318 1620 85 1071 3051
Fragments arranged by size:
3051 1620 1071 318 264 85
BsmI GAATG_Cn'
Cuts at: 2785 3006 3920 4013 2785
Size: 221 914 93 5181
Fragments arranged by size:
5181 914 221 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2268 2388 2532 2684 2835 2850
Size: 399 386 92 120 144 152 151 15
Cuts at: 2850 2914 3062 3607 3647 3728 4470 4555 5626
Size: 64 148 545 40 81 742 85 1071
Cuts at: 5626 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 545 399 386 152 151
148 144 120 92 85 81 64 40
15
BspEI T'CCGG_A
Cuts at: 2399 2605 3570 6072 2399
Size: 206 965 2502 2736
Fragments arranged by size:
2736 2502 965 206
BspHI T'CATG_A
Cuts at: 3021 4298 6028 3021
Size: 1277 1730 3402
Fragments arranged by size:
3402 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2539 2760 3574 3689 3764 844
Size: 1695 221 814 115 75 3489
Fragments arranged by size:
3489 1695 814 221 115 75
BsrBI CCG'CTC
Cuts at: 1394 1823 3511 4296 1394
Size: 429 1688 785 3507
Fragments arranged by size:
3507 1688 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2570 2646 5053 5240 1700
Size: 110 207 553 76 2407 187 2869
Fragments arranged by size:
2869 2407 553 207 187 110 76
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 4469 5481 4469
Size: 1012 5397
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6409
BstBI TT'CG_AA
Cuts at: 5213 5213
Size: 6409
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstYI r'GATC_y
Cuts at: 1972 3436 3739 4106 4491 4508 5960 6046 6058
Size: 1464 303 367 385 17 1452 86 12
Cuts at: 6058 6404 1972
Size: 346 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 303 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 2676 2994 3477 513
Size: 552 939 483 189 318 483 3445
Fragments arranged by size:
3445 939 552 483 483 318 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5670
Fragments arranged by size:
5670 683 56
BtsI GCAGTG_nn'
Cuts at: 3925 4719 4739 3925
Size: 794 20 5595
Fragments arranged by size:
5595 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6374
DraI TTT'AAA
Cuts at: 3713 3713
Size: 6409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5416 809
Size: 507 4100 1802
Fragments arranged by size:
4100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 3161 3474
Size: 54 1425 50 275 18 873 458 313
Cuts at: 3474 3856 4745 6374 8
Size: 382 889 1629 43
Fragments arranged by size:
1629 1425 889 873 458 382 313 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3474 3856 1537
Size: 275 1662 382 4090
Fragments arranged by size:
4090 1662 382 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2045 4339 1353
Size: 671 21 2294 3423
Fragments arranged by size:
3423 2294 671 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2530 3444 4996 5370 5516 387
Size: 1064 138 941 914 1552 374 146 1280
Fragments arranged by size:
1552 1280 1064 941 914 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2120 2249 2282 2836 3121 3169
Size: 393 458 30 129 33 554 285 48
Cuts at: 3169 3281 3508 3620 4472 5069 5856 6124 1239
Size: 112 227 112 852 597 787 268 1524
Fragments arranged by size:
1524 852 787 597 554 458 393 285
268 227 129 112 112 48 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 3638 2102
Size: 1536 4873
EcoO109I rG'GnC_Cy
Cuts at: 2172 2330 3058 3059 3643 3644 2172
Size: 158 728 1 584 1 4937
Fragments arranged by size:
4937 728 584 158 1 1
EcoRI G'AATT_C
Cuts at: 3718 4096 3718
Size: 378 6031
FspI TGC'GCA
Cuts at: 3531 4915 3531
Size: 1384 5025
HaeII r_GCGC'y
Cuts at: 1678 2228 2308 2419 3310 3557 5556 1678
Size: 550 80 111 891 247 1999 2531
Fragments arranged by size:
2531 1999 891 550 247 111 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 5050 5082 5124
Size: 32 135 32 1349 32 2097 32 42
Cuts at: 5124 5156 1373
Size: 32 2626
Fragments arranged by size:
2626 2097 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3753 3999 669
Size: 988 281 1815 246 3079
Fragments arranged by size:
3079 1815 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 3734 3734
Size: 6409
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 3186 5523 5707 1305
Size: 936 945 2337 184 2007
Fragments arranged by size:
2337 2007 945 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 10
Size: 54 1425 343 873 3714
Fragments arranged by size:
3714 1425 873 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4367 4726 4885 518
Size: 648 3201 359 159 2042
Fragments arranged by size:
3201 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2236 2489 2721 3321 3547 3825 4502
Size: 582 48 253 232 600 226 278 677
Cuts at: 4502 5650 5895 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 582 278 253 245
232 226 48
NaeI GCC'GGC
Cuts at: 2019 2019
Size: 6409
NcoI C'CATG_G
Cuts at: 513 1065 2004 2994 513
Size: 552 939 990 3928
Fragments arranged by size:
3928 990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NgoMIV G'CCGG_C
Cuts at: 2017 2017
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NsiI A_TGCA'T
Cuts at: 2455 2455
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 2152 3767 5312 1132
Size: 97 923 1615 1545 2229
Fragments arranged by size:
2229 1615 1545 923 97
PasI CC'CwG_GG
Cuts at: 1348 2727 2773 1348
Size: 1379 46 4984
Fragments arranged by size:
4984 1379 46
PciI A'CATG_T
Cuts at: 5308 5308
Size: 6409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6409
PfoI T'CCnGG_A
Cuts at: 3258 3636 3258
Size: 378 6031
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 3979 3979
Size: 6409
PspOMI G'GGCC_C
Cuts at: 2172 3058 3643 2172
Size: 886 585 4938
Fragments arranged by size:
4938 886 585
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 6377
PstI C_TGCA'G
Cuts at: 830 3057 3761 830
Size: 2227 704 3478
Fragments arranged by size:
3478 2227 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
PvuII CAG'CTG
Cuts at: 2188 3321 3547 2188
Size: 1133 226 5050
Fragments arranged by size:
5050 1133 226
SacII CC_GC'GG
Cuts at: 2490 2490
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SapI GCTCTTCn'nnn_
Cuts at: 2024 2024
Size: 6409
SbfI CC_TGCA'GG
Cuts at: 3057 3761 3057
Size: 704 5705
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SfcI C'TryA_G
Cuts at: 826 1045 3053 3757 4892 5573 5764 826
Size: 219 2008 704 1135 681 191 1471
Fragments arranged by size:
2008 1471 1135 704 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 2909 3291 3736 1872
Size: 1037 382 445 4545
Fragments arranged by size:
4545 1037 445 382
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2693 4513 5414 5676 820
Size: 197 926 750 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 750 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SrfI GCCC'GGGC
Cuts at: 3291 3291
Size: 6409
SspI AAT'ATT
Cuts at: 5 52 4333 5
Size: 47 4281 2081
Fragments arranged by size:
4281 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6409
StyI C'CwwG_G
Cuts at: 513 1065 2004 2352 2700 2994 3848 513
Size: 552 939 348 348 294 854 3074
Fragments arranged by size:
3074 939 854 552 348 348 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 4436 4453 4606 4791 5236 325
Size: 1255 1196 1660 17 153 185 445 1498
Fragments arranged by size:
1660 1498 1255 1196 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 4655 6367
Size: 274 80 33 51 1443 159 2519 1712
Cuts at: 6367 96
Size: 138
Fragments arranged by size:
2519 1712 1443 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 3200 3227 3232 3259 3583 3615 6369
Size: 32 646 27 5 27 324 32 2754
Cuts at: 6369 6401 2522
Size: 32 2530
Fragments arranged by size:
2754 2530 646 324 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 3491 1316
Size: 960 1215 4234
Fragments arranged by size:
4234 1215 960
XbaI T'CTAG_A
Cuts at: 3745 3745
Size: 6409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 3097 3644 2224
Size: 873 547 4989
Fragments arranged by size:
4989 873 547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 2907 3289 3734 1870
Size: 1037 382 445 4545
Fragments arranged by size:
4545 1037 445 382
XmnI GAAnn'nnTTC
Cuts at: 4538 4538
Size: 6409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspXI RsrII SacI SanDI SexAI SfiI SfoI
SwaI