Restriction map for
pFN21AE0720
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AvaI AviII AvrII BalI BanII
BbeI BbsI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsmBI BsmI BsoBI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 416 GGTACC G^GTACC
AccI 616 GTCGAC GT^[AC][GT]AC
AcsI 1081 GAATTC [GA]^AATT[CT]
AhdI 619 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 70 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1081 GAATTC [GA]^AATT[CT]
Asp700I 198 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 416 GGTACC G^GTACC
AspEI 619 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 235 GAGCAC G[AT]GC[AT]^C
BamHI 795 GGATCC G^GATCC
BbrPI 747 CACGTG CAC^GTG
BbvCI 774 GCTGAGG CCTCAGC(-5/-2)
BciVI 601 GGATAC GTATCC(6/5)
BfaI 958 CTAG C^TAG
BsaAI 747 CACGTG [CT]AC^GT[GA]
BsiHKAI 235 GAGCAC G[AT]GC[AT]^C
BsiWI 671 CGTACG C^GTACG
Bsp1407I 581 TGTACA T^GTACA
BspDI 697 ATCGAT AT^CGAT
BspHI 822 TCATGA T^CATGA
BsrDI 148 GCAATG GCAATG(2/0)
BsrGI 581 TGTACA T^GTACA
CfoI 324 GCGC GCG^C
ClaI 697 ATCGAT AT^CGAT
DraII 594 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 747 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 848 TGGCCG [CT]^GGCC[GA]
Eam1105I 619 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 946 GAAGAG CTCTTC(1/4)
EcoNI 786 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 594 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1081 GAATTC G^AATTC
EcoT22I 111 ATGCAT ATGCA^T
HgaI 865 GACGC GACGC(5/10)
HhaI 324 GCGC GCG^C
HinP1I 324 GCGC G^CGC
HphI 1211 TCACC GGTGA(8/7)
KpnI 416 GGTACC GGTAC^C
Ksp632I 946 GAAGAG CTCTTC(1/4)
MaeI 958 CTAG C^TAG
MaeII 748 ACGT A^CGT
MaeIII 688 GTCAC ^GT[ACGT]AC
NcoI 536 CCATGG C^CATGG
NsiI 111 ATGCAT ATGCA^T
PflMI 1134 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 747 CACGTG CAC^GTG
PmlI 747 CACGTG CAC^GTG
PvuII 1029 CAGCTG CAG^CTG
RcaI 822 TCATGA T^CATGA
SalI 616 GTCGAC G^TCGAC
SspBI 581 TGTACA T^GTACA
Tsp45I 688 GTCAC ^GT[GC]AC
Tsp509I 1082 AATT ^AATT
Van91I 1134 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 957 TCTAGA T^CTAGA
XcmI 548 CCAAGCCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 198 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 808 CGCG CG^CG
867 CGCG
AvaII 300 GGACC G^G[AT]CC
626 GGTCC
BanI 416 GGTACC G^G[CT][GA]CC
544 GGCACC
BsgI 1221 CTGCAC GTGCAG(16/14)
118 GTGCAG
BstAPI 216 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
339 GCAGAATGTGC
BstUI 808 CGCG CG^CG
867 CGCG
BstYI 795 GGATCC [GA]^GATC[CT]
1006 AGATCC
DsaI 536 CCATGG C^C[GA][CT]GG
1227 CCGTGG
EcoT14I 536 CCATGG C^C[AT][AT]GG
791 CCAAGG
HincII 52 GTCAAC GT[CT]^[GA]AC
616 GTCGAC
HindII 52 GTCAAC GT[CT]^[GA]AC
616 GTCGAC
MflI 795 GGATCC [GA]^GATC[CT]
1006 AGATCC
MspA1I 1029 CAGCTG C[AC]G^C[GT]G
1036 CCGCTG
MvnI 808 CGCG CG^CG
867 CGCG
StyI 536 CCATGG C^C[AT][AT]GG
791 CCAAGG
XhoII 795 GGATCC [GA]^GATC[CT]
1006 AGATCC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI TspRI TthHB8I