Restriction map for
pFN21AE0720
(Circular) (Six-base) MAPSORT of: pFN21AE0720.seq Check: 8291 from: 1 to: 5947
pFN21AE0720 5947 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3063 3233 3063
Size: 170 5777
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 3290 1937
Size: 685 668 4594
Fragments arranged by size:
4594 685 668
Acc65I G'GTAC_C
Cuts at: 2421 3268 2421
Size: 847 5100
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 4010 5394 5662 1632
Size: 767 1611 1384 268 1917
Fragments arranged by size:
1917 1611 1384 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2289 2479 2718 4846 5823 1203
Size: 1086 190 239 2128 977 1327
Fragments arranged by size:
2128 1327 1086 977 239 190
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 2629 4676 2629
Size: 2047 3900
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2861 2893 2276
Size: 32 553 32 5330
Fragments arranged by size:
5330 553 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2080 3690 5262 2080
Size: 1610 1572 2765
Fragments arranged by size:
2765 1610 1572
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 3086 3256 3482 3634 5743 1493
Size: 1593 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 1593 226 170 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 2800 3277 3644 2800
Size: 477 367 5103
Fragments arranged by size:
5103 477 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 2549 3268 4723 618
Size: 325 217 627 634 128 719 1455 1842
Fragments arranged by size:
1842 1455 719 634 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BbvCI CC'TCA_GC
Cuts at: 2780 2780
Size: 5947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2600 3839 5049 2600
Size: 1239 1210 3498
Fragments arranged by size:
3498 1239 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BfrBI ATG'CAT
Cuts at: 2118 2118
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2847 2910 4008 5164 1790
Size: 1057 63 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 1057 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2875 3678 4636
Size: 416 53 83 399 24 1458 803 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1458 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2426 2553 2689 3173 4607 1239
Size: 1019 168 127 136 484 1434 2579
Fragments arranged by size:
2579 1434 1019 484 168 136 127
Bpu10I CC'TnA_GC
Cuts at: 2780 3113 2780
Size: 333 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2754 3738 493
Size: 711 1550 984 2702
Fragments arranged by size:
2702 1550 984 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 2732 2762 1178
Size: 30 165 30 873 30 426 30 4363
Fragments arranged by size:
4363 873 426 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2737 3203 1186
Size: 1111 440 466 3930
Fragments arranged by size:
3930 1111 466 440
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 3211 1163
Size: 573 408 1067 3899
Fragments arranged by size:
3899 1067 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 4008 4093 5164 2244
Size: 1764 85 1071 3027
Fragments arranged by size:
3027 1764 1071 85
BsiWI C'GTAC_G
Cuts at: 2676 2676
Size: 5947
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2847 2910 3266 4008 4093 5164
Size: 399 454 603 63 356 742 85 1071
Cuts at: 5164 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 603 454 399 356 85
63
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 2827 3836 5566 2827
Size: 1009 1730 3208
Fragments arranged by size:
3208 1730 1009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2334 3063 3233 3302 844
Size: 1472 18 729 170 69 3489
Fragments arranged by size:
3489 1472 729 170 69 18
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2160 4442 4616 65
Size: 1183 912 2282 174 1396
Fragments arranged by size:
2282 1396 1183 912 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 2586 96
Size: 2490 3457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 2350 794
Size: 1433 123 4391
Fragments arranged by size:
4391 1433 123
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 2800 3011 3277 3644 4029 4046 5498 5584
Size: 828 211 266 367 385 17 1452 86
Cuts at: 5584 5596 5942 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 828 385 367 346 266 211
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2541 3232 513
Size: 552 939 537 691 3228
Fragments arranged by size:
3228 939 691 552 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 2703 1554
Size: 1149 4798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DraIII CAC_nnn'GTG
Cuts at: 2757 2757
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2853 3394 4283
Size: 54 1425 50 275 18 1023 541 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1023 889 541 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3877 1353
Size: 1593 931 3423
Fragments arranged by size:
3423 1593 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2399 2426 2553 2689 3173 4010
Size: 393 626 141 27 127 136 484 837
Cuts at: 4010 4607 5394 5662 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 837 787 626 597 484 393 268
141 136 127 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2795 2795
Size: 5947
EcoO109I rG'GnC_Cy
Cuts at: 2600 2600
Size: 5947
EcoRI G'AATT_C
Cuts at: 3086 3256 3634 3086
Size: 170 378 5399
Fragments arranged by size:
5399 378 170
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2615 2647 2785 2817 4588
Size: 32 135 32 1043 32 138 32 1771
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1771 1043 138 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2623 3291 3537 669
Size: 988 281 121 564 668 246 3079
Fragments arranged by size:
3079 988 668 564 281 246 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 2425 3272 2425
Size: 847 5100
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2300 2478 3905 4264 4423 518
Size: 648 1128 6 178 1427 359 159 2042
Fragments arranged by size:
2042 1427 1128 648 359 178 159 6
MspA1I CmG'CkG
Cuts at: 1606 3036 3043 3363 4040 5188 5433 1606
Size: 1430 7 320 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 320 245 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2541 513
Size: 552 939 537 3919
Fragments arranged by size:
3919 939 552 537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NsiI A_TGCA'T
Cuts at: 2120 2120
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2293 2483 2722 3305 4850 1132
Size: 97 1064 190 239 583 1545 2229
Fragments arranged by size:
2229 1545 1064 583 239 190 97
PasI CC'CwG_GG
Cuts at: 1348 2635 1348
Size: 1287 4660
PciI A'CATG_T
Cuts at: 2289 2479 2718 4846 2289
Size: 190 239 2128 3390
Fragments arranged by size:
3390 2128 239 190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3145 1108
Size: 2037 3910
PfoI T'CCnGG_A
Cuts at: 2211 2274 3078 3189 2211
Size: 63 804 111 4969
Fragments arranged by size:
4969 804 111 63
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PmlI CAC'GTG
Cuts at: 2754 2754
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2308 4016 4048 5529 5561 2276
Size: 32 1708 32 1481 32 2662
Fragments arranged by size:
2662 1708 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 2223 2343 3060 3242 3299 830
Size: 1393 120 717 182 57 3478
Fragments arranged by size:
3478 1393 717 182 120 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 3036 3036
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 2621 3289 1936
Size: 685 668 4594
Fragments arranged by size:
4594 685 668
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2219 2339 3056 3238 3295 4430 5111
Size: 219 1174 120 717 182 57 1135 681
Cuts at: 5111 5302 826
Size: 191 1471
Fragments arranged by size:
1471 1174 1135 717 681 219 191 182
120 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2004 2541 2796 3386 513
Size: 552 939 537 255 590 3074
Fragments arranged by size:
3074 939 590 552 537 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 3233 3974 3991 4144 4329 4774
Size: 1255 1100 553 741 17 153 185 445
Cuts at: 4774 325
Size: 1498
Fragments arranged by size:
1498 1255 1100 741 553 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2586 4193
Size: 274 80 33 51 1443 183 426 1607
Cuts at: 4193 5905 96
Size: 1712 138
Fragments arranged by size:
1712 1607 1443 426 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 5907 5939 2732
Size: 32 3143 32 2740
Fragments arranged by size:
3143 2740 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 2962 3283 2962
Size: 321 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 4076 2207
Size: 1869 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI