Restriction map for
pFN21AE0720
(Linear) (Six-base) MAPSORT of: KIEE0720.seq Check: 7311 from: 1 to: 1242
KIEE0720.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1058 1228 1242
Size: 1058 170 14
Fragments arranged by size:
1058 170 14
AccI GT'mk_AC
Cuts at: 0 617 1242
Size: 617 625
Acc65I G'GTAC_C
Cuts at: 0 416 1242
Size: 416 826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1242
Size: 394 848
AflIII A'CryG_T
Cuts at: 0 284 474 713 1242
Size: 284 190 239 529
Fragments arranged by size:
529 284 239 190
AhdI GACnn_n'nnGTC
Cuts at: 0 624 1242
Size: 624 618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 856 888 1242
Size: 271 32 553 32 354
Fragments arranged by size:
553 354 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 75 1242
Size: 75 1167
ApoI r'AATT_y
Cuts at: 0 1081 1242
Size: 1081 161
BamHI G'GATC_C
Cuts at: 0 795 1242
Size: 795 447
BanI G'GyrC_C
Cuts at: 0 416 544 1242
Size: 416 128 698
Fragments arranged by size:
698 416 128
BbvCI CC'TCA_GC
Cuts at: 0 775 1242
Size: 775 467
BciVI GTATCCnnnnn_n'
Cuts at: 0 595 1242
Size: 595 647
BfrBI ATG'CAT
Cuts at: 0 113 1242
Size: 113 1129
Bme1580I G_kGCm'C
Cuts at: 0 842 905 1242
Size: 842 63 337
Fragments arranged by size:
842 337 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 870 1242
Size: 870 372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1242
Size: 1123 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 421 548 684 1168 1242
Size: 253 168 127 136 484 74
Fragments arranged by size:
484 253 168 136 127 74
Bpu10I CC'TnA_GC
Cuts at: 0 775 1108 1242
Size: 775 333 134
Fragments arranged by size:
775 333 134
BsaAI yAC'GTr
Cuts at: 0 749 1242
Size: 749 493
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 727 757 1242
Size: 271 30 426 30 485
Fragments arranged by size:
485 426 271 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 732 1198 1242
Size: 292 440 466 44
Fragments arranged by size:
466 440 292 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1206 1242
Size: 139 1067 36
Fragments arranged by size:
1067 139 36
BsiHKAI G_wGCw'C
Cuts at: 0 239 1242
Size: 239 1003
BsiWI C'GTAC_G
Cuts at: 0 671 1242
Size: 671 571
Bsp1286I G_dGCh'C
Cuts at: 0 239 842 905 1242
Size: 239 603 63 337
Fragments arranged by size:
603 337 239 63
BspHI T'CATG_A
Cuts at: 0 822 1242
Size: 822 420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 329 1058 1228 1242
Size: 311 18 729 170 14
Fragments arranged by size:
729 311 170 18 14
BsrDI GCAATG_nn'
Cuts at: 0 155 1242
Size: 155 1087
BsrGI T'GTAC_A
Cuts at: 0 581 1242
Size: 581 661
BstAPI GCAn_nnn'nTGC
Cuts at: 0 222 345 1242
Size: 222 123 897
Fragments arranged by size:
897 222 123
BstYI r'GATC_y
Cuts at: 0 795 1006 1242
Size: 795 211 236
Fragments arranged by size:
795 236 211
BtgI C'CryG_G
Cuts at: 0 536 1227 1242
Size: 536 691 15
Fragments arranged by size:
691 536 15
ClaI AT'CG_AT
Cuts at: 0 698 1242
Size: 698 544
DraIII CAC_nnn'GTG
Cuts at: 0 752 1242
Size: 752 490
EaeI y'GGCC_r
Cuts at: 0 848 1242
Size: 848 394
EarI CTCTTCn'nnn_
Cuts at: 0 941 1242
Size: 941 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 394 421 548 684 1168 1242
Size: 253 141 27 127 136 484 74
Fragments arranged by size:
484 253 141 136 127 74 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 790 1242
Size: 790 452
EcoO109I rG'GnC_Cy
Cuts at: 0 595 1242
Size: 595 647
EcoRI G'AATT_C
Cuts at: 0 1081 1242
Size: 1081 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 780 812 1242
Size: 610 32 138 32 430
Fragments arranged by size:
610 430 138 32 32
HincII GTy'rAC
Cuts at: 0 54 618 1242
Size: 54 564 624
Fragments arranged by size:
624 564 54
KpnI G_GTAC'C
Cuts at: 0 420 1242
Size: 420 822
MslI CAynn'nnrTG
Cuts at: 0 289 295 473 1242
Size: 289 6 178 769
Fragments arranged by size:
769 289 178 6
MspA1I CmG'CkG
Cuts at: 0 1031 1038 1242
Size: 1031 7 204
Fragments arranged by size:
1031 204 7
NcoI C'CATG_G
Cuts at: 0 536 1242
Size: 536 706
NsiI A_TGCA'T
Cuts at: 0 115 1242
Size: 115 1127
NspI r_CATG'y
Cuts at: 0 288 478 717 1242
Size: 288 190 239 525
Fragments arranged by size:
525 288 239 190
PasI CC'CwG_GG
Cuts at: 0 630 1242
Size: 630 612
PciI A'CATG_T
Cuts at: 0 284 474 713 1242
Size: 284 190 239 529
Fragments arranged by size:
529 284 239 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 1140 1242
Size: 1140 102
PfoI T'CCnGG_A
Cuts at: 0 206 269 1073 1184 1242
Size: 206 63 804 111 58
Fragments arranged by size:
804 206 111 63 58
PmlI CAC'GTG
Cuts at: 0 749 1242
Size: 749 493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1242
Size: 271 32 939
Fragments arranged by size:
939 271 32
PstI C_TGCA'G
Cuts at: 0 218 338 1055 1237 1242
Size: 218 120 717 182 5
Fragments arranged by size:
717 218 182 120 5
PvuII CAG'CTG
Cuts at: 0 1031 1242
Size: 1031 211
SalI G'TCGA_C
Cuts at: 0 616 1242
Size: 616 626
SfcI C'TryA_G
Cuts at: 0 214 334 1051 1233 1242
Size: 214 120 717 182 9
Fragments arranged by size:
717 214 182 120 9
StyI C'CwwG_G
Cuts at: 0 536 791 1242
Size: 536 255 451
Fragments arranged by size:
536 451 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 1228 1242
Size: 675 553 14
Fragments arranged by size:
675 553 14
TatI w'GTAC_w
Cuts at: 0 155 581 1242
Size: 155 426 661
Fragments arranged by size:
661 426 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 1242
Size: 727 32 483
Fragments arranged by size:
727 483 32
XbaI T'CTAG_A
Cuts at: 0 957 1242
Size: 957 285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1242
Size: 555 687
XmnI GAAnn'nnTTC
Cuts at: 0 202 1242
Size: 202 1040
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AloI AlwNI
ApoI BamHI BanI BbvCI BciVI BfrBI Bme1580I BmrI
BplI BpmI Bpu10I BsaAI BsaXI BseRI BsgI BsiHKAI
BsiWI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstYI
BtgI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI Hin4I HincII KpnI MslI MspA1I NcoI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PstI
PvuII SalI SfcI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII
BbeI BbsI BcgI BclI BglI BglII BlpI BmgBI
BmtI BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsiEI
BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DraI
DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI ZraI