Restriction map for
pFN21AE0712
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 492 GACGTC GACGT^C
AfaI 882 GTAC GT^AC
AflIII 345 ACATGT A^C[GA][CT]GT
AlwI 953 GGATC GGATC(4/5)
ApaI 874 GGGCCC GGGCC^C
Asp700I 150 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 604 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 165 CTCGGG C^[CT]CG[GA]G
BbsI 349 GTCTTC GAAGAC(2/6)
BfaI 362 CTAG C^TAG
BpuAI 349 GTCTTC GAAGAC(2/6)
BsaI 616 GAGACC GGTCTC(1/5)
BsaWI 30 ACCGGA [AT]^CCGG[AT]
BsgI 328 CTGCAC GTGCAG(16/14)
BsiEI 1005 CGACCG CG[GA][CT]^CG
BsmBI 66 CGTCTC CGTCTC(1/5)
BsoBI 165 CTCGGG C^[CT]CG[GA]G
BspLU11I 345 ACATGT A^CATGT
BsrDI 477 GCAATG GCAATG(2/0)
EaeI 41 CGGCCA [CT]^GGCC[GA]
EarI 825 GAAGAG CTCTTC(1/4)
HincII 1213 GTCGAC GT[CT]^[GA]AC
HindII 1213 GTCGAC GT[CT]^[GA]AC
Ksp632I 825 GAAGAG CTCTTC(1/4)
MaeI 362 CTAG C^TAG
NheI 361 GCTAGC G^CTAGC
NspI 345 ACATGT [GA]CATG^[CT]
PflFI 604 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 577 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 937 CTGCAG CTGCA^G
RsaI 882 GTAC GT^AC
SacI 175 GAGCTC GAGCT^C
SalI 1213 GTCGAC G^TCGAC
SfcI 937 CTGCAG C^T[GA][CT]AG
TaqI 1214 TCGA T^CGA
TfiI 98 GATTC G^A[AT]TC
Tsp45I 764 GTGAC ^GT[GC]AC
Tsp509I 1030 AATT ^AATT
Tth111I 604 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1214 TCGA T^CGA
Van91I 577 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 150 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 610 GTCTAC GT^[AC][GT]AC
1213 GTCGAC
AccII 39 CGCG CG^CG
1177 CGCG
Aor51HI 104 AGCGCT AGC^GCT
309 AGCGCT
AspHI 175 GAGCTC G[AT]GC[AT]^C
453 GTGCTC
BanI 631 GGCACC G^G[CT][GA]CC
1206 GGTGCC
BcnI 1008 CCGGG CC^[GC]GG
1088 CCGGG
BglII 464 AGATCT A^GATCT
1130 AGATCT
BsiHKAI 175 GAGCTC G[AT]GC[AT]^C
453 GTGCTC
BsmAI 616 GAGAC GTCTC(1/5)
67 GTCTC
BsrBI 654 CCGCTC CCGCTC(-3/-3)
703 GAGCGG
BstUI 39 CGCG CG^CG
1177 CGCG
BstYI 464 AGATCT [GA]^GATC[CT]
1130 AGATCT
Eco47III 104 AGCGCT AGC^GCT
309 AGCGCT
EcoNI 248 CCTCGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
858 CCTGCGGAAGG
FauI 705 GCGGG CCCGC(4/6)
1197 GCGGG
HphI 763 GGTGA GGTGA(8/7)
1044 GGTGA
MaeII 493 ACGT A^CGT
890 ACGT
MaeIII 764 GTGAC ^GT[ACGT]AC
1106 GTTAC
MflI 464 AGATCT [GA]^GATC[CT]
1130 AGATCT
MslI 189 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
957 CATGCTGGTG
MvnI 39 CGCG CG^CG
1177 CGCG
NciI 1008 CCGGG CC^[GC]GG
1088 CCGGG
XhoII 464 AGATCT [GA]^GATC[CT]
1130 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AluI AvaII BanII
BbvI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SmlI TseI
TspRI