(Circular) (Six-base) MAPSORT of: pFN21AE0712.seq Check: 1901 from: 1 to: 5938 pFN21AE0712 5938 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2501 3748 278 Size: 53 83 186 936 965 1247 2468 Fragments arranged by size: 2468 1247 965 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2616 3219 3281 1937 Size: 679 603 62 4594 Fragments arranged by size: 4594 679 603 62 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2506 4001 5385 5653 1632 Size: 874 1495 1384 268 1917 Fragments arranged by size: 1917 1495 1384 874 268 AfeI AGC'GCT Cuts at: 2111 2316 2111 Size: 205 5733 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 2350 4837 5814 1203 Size: 1147 2487 977 1327 Fragments arranged by size: 2487 1327 1147 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 2198 1166 Size: 1032 4906 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 3681 5253 3681 Size: 1572 4366 ApaI G_GGCC'C Cuts at: 2883 2883 Size: 5938 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2170 3263 1870 Size: 73 227 1093 4545 Fragments arranged by size: 4545 1093 227 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2636 3211 3259 4714 618 Size: 325 217 627 849 575 48 1455 1842 Fragments arranged by size: 1842 1455 849 627 575 325 217 48 BanII G_rGCy'C Cuts at: 1391 2177 2184 2883 3257 1391 Size: 786 7 699 374 4072 Fragments arranged by size: 4072 786 699 374 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2347 928 Size: 971 448 4519 Fragments arranged by size: 4519 971 448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 5040 3830 Size: 1210 4728 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2469 3135 5933 2469 Size: 666 2798 2474 Fragments arranged by size: 2798 2474 666 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2192 2883 3999 5155 1790 Size: 402 691 1116 1156 2573 Fragments arranged by size: 2573 1156 1116 691 402 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2288 2598 3669 Size: 416 53 83 399 24 871 310 1071 Cuts at: 3669 4627 442 Size: 958 1753 Fragments arranged by size: 1753 1071 958 871 416 399 310 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2370 1056 Size: 1314 4624 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2081 2647 2766 2970 4598 1239 Size: 827 15 566 119 204 1628 2579 Fragments arranged by size: 2579 1628 827 566 204 119 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 2168 3107 4063 4928 5226 2108 Size: 60 939 956 865 298 2820 Fragments arranged by size: 2820 956 939 865 298 60 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 4601 882 Size: 617 1116 1986 2219 Fragments arranged by size: 2219 1986 1116 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2498 3222 3745 Size: 53 83 186 936 677 288 724 523 Cuts at: 3745 4127 4762 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 724 677 635 523 382 288 186 83 53 BsaWI w'CCGG_w Cuts at: 2035 4371 4769 5043 5190 5601 2035 Size: 2336 398 274 147 411 2372 Fragments arranged by size: 2372 2336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2689 2719 2832 2862 1178 Size: 30 165 30 1286 30 113 30 4254 Fragments arranged by size: 4254 1286 165 113 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2771 2840 1186 Size: 1294 291 69 4284 Fragments arranged by size: 4284 1294 291 69 BseYI C'CCAG_C Cuts at: 2797 3028 5141 5637 2797 Size: 231 2113 496 3098 Fragments arranged by size: 3098 2113 496 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 1163 Size: 573 582 4783 Fragments arranged by size: 4783 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3388 4149 5177 1540 Size: 275 186 1012 375 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 375 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2462 3999 4084 5155 2184 Size: 278 1537 85 1071 2967 Fragments arranged by size: 2967 1537 1071 278 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 BsmBI CGTCTCn'nnnn_ Cuts at: 2077 2077 Size: 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2184 2192 2462 2883 3257 3999 Size: 399 387 7 8 270 421 374 742 Cuts at: 3999 4084 5155 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 387 374 270 85 8 7 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 844 Size: 2449 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2661 2710 3825 1394 Size: 429 838 49 1115 3507 Fragments arranged by size: 3507 1115 838 429 49 BsrDI GCAATG_nn' Cuts at: 65 1248 2489 4433 4607 65 Size: 1183 1241 1944 174 1396 Fragments arranged by size: 1944 1396 1241 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2469 3135 3268 3635 4020 4037 5489 5575 Size: 497 666 133 367 385 17 1452 86 Cuts at: 5575 5587 5933 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 666 497 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2437 487 Size: 683 56 1211 3988 Fragments arranged by size: 3988 1211 683 56 BtsI GCAGTG_nn' Cuts at: 2222 3196 3454 4248 4268 2222 Size: 974 258 794 20 3892 Fragments arranged by size: 3892 974 794 258 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3385 4274 Size: 54 1425 50 275 18 216 1339 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1339 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 3868 1353 Size: 1472 1043 3423 Fragments arranged by size: 3423 1472 1043 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 2501 4525 4899 5045 387 Size: 1064 138 476 436 2024 374 146 1280 Fragments arranged by size: 2024 1280 1064 476 436 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2182 Size: 5938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2081 2506 2647 2766 2970 4001 Size: 393 434 15 425 141 119 204 1031 Cuts at: 4001 4598 5385 5653 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1031 787 597 434 425 393 268 204 141 119 15 EcoNI CCTnn'n_nnAGG Cuts at: 2257 2867 2257 Size: 610 5328 EcoO109I rG'GnC_Cy Cuts at: 2263 2762 2879 3205 2263 Size: 499 117 326 4996 Fragments arranged by size: 4996 499 326 117 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 2113 2318 3076 5085 1678 Size: 435 205 758 2009 2531 Fragments arranged by size: 2531 2009 758 435 205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 2832 2864 2943 Size: 32 135 32 1190 32 38 32 79 Cuts at: 2943 2975 4579 4611 4653 4685 1373 Size: 32 1604 32 42 32 2626 Fragments arranged by size: 2626 1604 1190 135 79 42 38 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3220 3282 3528 669 Size: 988 281 1282 62 246 3079 Fragments arranged by size: 3079 1282 988 281 246 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5052 5236 1305 Size: 3747 184 2007 Fragments arranged by size: 3747 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2966 3896 4255 4414 518 Size: 648 1032 768 930 359 159 2042 Fragments arranged by size: 2042 1032 930 768 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3354 4031 5179 5424 1606 Size: 1748 677 1148 245 2120 Fragments arranged by size: 2120 1748 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 2366 1052 Size: 1314 4624 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2354 3296 4841 1132 Size: 97 1125 942 1545 2229 Fragments arranged by size: 2229 1545 1125 942 97 PasI CC'CwG_GG Cuts at: 1348 2063 1348 Size: 715 5223 PciI A'CATG_T Cuts at: 2350 4837 2350 Size: 2487 3451 PflMI CCAn_nnn'nTGG Cuts at: 1108 2588 1108 Size: 1480 4458 PfoI T'CCnGG_A Cuts at: 2082 2343 2836 3092 2082 Size: 261 493 256 4928 Fragments arranged by size: 4928 493 261 256 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PspOMI G'GGCC_C Cuts at: 2879 2879 Size: 5938 PstI C_TGCA'G Cuts at: 830 2946 3290 830 Size: 2116 344 3478 Fragments arranged by size: 3478 2116 344 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 SacI G_AGCT'C Cuts at: 2184 2184 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3218 3280 1936 Size: 1282 62 4594 Fragments arranged by size: 4594 1282 62 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2942 3286 4421 5102 5293 826 Size: 219 1897 344 1135 681 191 1471 Fragments arranged by size: 1897 1471 1135 681 344 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2123 2183 3122 4042 4943 5205 Size: 197 926 180 60 939 920 901 262 Cuts at: 5205 820 Size: 1553 Fragments arranged by size: 1553 939 926 920 901 262 197 180 60 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 3377 513 Size: 552 2312 3074 Fragments arranged by size: 3074 2312 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2066 2260 2599 2738 3965 3982 Size: 1255 444 42 194 339 139 1227 17 Cuts at: 3982 4135 4320 4765 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1227 445 444 339 194 185 153 139 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2886 4184 5896 Size: 274 80 33 51 1443 909 1298 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1712 1443 1298 909 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2689 2721 5898 5930 2689 Size: 32 3177 32 2697 Fragments arranged by size: 3177 2697 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2612 1316 Size: 1296 4642 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2159 4067 2159 Size: 1908 4030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2499 3746 276 Size: 53 83 186 936 965 1247 2468 Fragments arranged by size: 2468 1247 965 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI