Restriction map for
pFN21AE0712
(Linear) (Six-base) MAPSORT of: KIEE0712.seq Check: 8164 from: 1 to: 1233
KIEE0712.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 0 496 1233
Size: 496 737
AccI GT'mk_AC
Cuts at: 0 611 1214 1233
Size: 611 603 19
Fragments arranged by size:
611 603 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 1233
Size: 501 732
AfeI AGC'GCT
Cuts at: 0 106 311 1233
Size: 106 205 922
Fragments arranged by size:
922 205 106
AflIII A'CryG_T
Cuts at: 0 345 1233
Size: 345 888
AleI CACnn'nnGTG
Cuts at: 0 193 1233
Size: 193 1040
ApaI G_GGCC'C
Cuts at: 0 878 1233
Size: 878 355
AvaI C'yCGr_G
Cuts at: 0 165 1233
Size: 165 1068
BanI G'GyrC_C
Cuts at: 0 631 1206 1233
Size: 631 575 27
Fragments arranged by size:
631 575 27
BanII G_rGCy'C
Cuts at: 0 172 179 878 1233
Size: 172 7 699 355
Fragments arranged by size:
699 355 172 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 1233
Size: 342 891
BglII A'GATC_T
Cuts at: 0 464 1130 1233
Size: 464 666 103
Fragments arranged by size:
666 464 103
Bme1580I G_kGCm'C
Cuts at: 0 187 878 1233
Size: 187 691 355
Fragments arranged by size:
691 355 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 593 1233
Size: 283 310 640
Fragments arranged by size:
640 310 283
BmtI G_CTAG'C
Cuts at: 0 365 1233
Size: 365 868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 483 1233
Size: 483 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 76 642 761 965 1233
Size: 61 15 566 119 204 268
Fragments arranged by size:
566 268 204 119 61 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 163 1102 1233
Size: 103 60 939 131
Fragments arranged by size:
939 131 103 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 1233
Size: 610 623
BsaHI Gr'CG_yC
Cuts at: 0 205 493 1217 1233
Size: 205 288 724 16
Fragments arranged by size:
724 288 205 16
BsaWI w'CCGG_w
Cuts at: 0 30 1233
Size: 30 1203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 684 714 827 857 1233
Size: 684 30 113 30 376
Fragments arranged by size:
684 376 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 766 835 1233
Size: 475 291 69 398
Fragments arranged by size:
475 398 291 69
BseYI C'CCAG_C
Cuts at: 0 792 1023 1233
Size: 792 231 210
Fragments arranged by size:
792 231 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1233
Size: 313 920
BsiEI CG_ry'CG
Cuts at: 0 1008 1233
Size: 1008 225
BsiHKAI G_wGCw'C
Cuts at: 0 179 457 1233
Size: 179 278 776
Fragments arranged by size:
776 278 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 72 1233
Size: 72 1161
Bsp1286I G_dGCh'C
Cuts at: 0 172 179 187 457 878 1233
Size: 172 7 8 270 421 355
Fragments arranged by size:
421 355 270 172 8 7
BsrBI CCG'CTC
Cuts at: 0 656 705 1233
Size: 656 49 528
Fragments arranged by size:
656 528 49
BsrDI GCAATG_nn'
Cuts at: 0 484 1233
Size: 484 749
BstYI r'GATC_y
Cuts at: 0 464 1130 1233
Size: 464 666 103
Fragments arranged by size:
666 464 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 1233
Size: 432 801
BtsI GCAGTG_nn'
Cuts at: 0 217 1191 1233
Size: 217 974 42
Fragments arranged by size:
974 217 42
EaeI y'GGCC_r
Cuts at: 0 41 1233
Size: 41 1192
EarI CTCTTCn'nnn_
Cuts at: 0 820 1233
Size: 820 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 496 1233
Size: 60 436 737
Fragments arranged by size:
737 436 60
EcoICRI GAG'CTC
Cuts at: 0 177 1233
Size: 177 1056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 76 501 642 761 965 1233
Size: 61 15 425 141 119 204 268
Fragments arranged by size:
425 268 204 141 119 61 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 252 862 1233
Size: 252 610 371
Fragments arranged by size:
610 371 252
EcoO109I rG'GnC_Cy
Cuts at: 0 258 757 874 1200 1233
Size: 258 499 117 326 33
Fragments arranged by size:
499 326 258 117 33
HaeII r_GCGC'y
Cuts at: 0 108 313 1071 1233
Size: 108 205 758 162
Fragments arranged by size:
758 205 162 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 827 859 938 970 1233
Size: 757 32 38 32 79 32 263
Fragments arranged by size:
757 263 79 38 32 32 32
HincII GTy'rAC
Cuts at: 0 1215 1233
Size: 1215 18
MslI CAynn'nnrTG
Cuts at: 0 193 961 1233
Size: 193 768 272
Fragments arranged by size:
768 272 193
NheI G'CTAG_C
Cuts at: 0 361 1233
Size: 361 872
NspI r_CATG'y
Cuts at: 0 349 1233
Size: 349 884
PasI CC'CwG_GG
Cuts at: 0 58 1233
Size: 58 1175
PciI A'CATG_T
Cuts at: 0 345 1233
Size: 345 888
PflMI CCAn_nnn'nTGG
Cuts at: 0 583 1233
Size: 583 650
PfoI T'CCnGG_A
Cuts at: 0 77 338 831 1087 1233
Size: 77 261 493 256 146
Fragments arranged by size:
493 261 256 146 77
PspOMI G'GGCC_C
Cuts at: 0 874 1233
Size: 874 359
PstI C_TGCA'G
Cuts at: 0 941 1233
Size: 941 292
SacI G_AGCT'C
Cuts at: 0 179 1233
Size: 179 1054
SalI G'TCGA_C
Cuts at: 0 1213 1233
Size: 1213 20
SfcI C'TryA_G
Cuts at: 0 937 1233
Size: 937 296
SmlI C'TyrA_G
Cuts at: 0 118 178 1117 1233
Size: 118 60 939 116
Fragments arranged by size:
939 118 116 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 61 255 594 733 1233
Size: 19 42 194 339 139 500
Fragments arranged by size:
500 339 194 139 42 19
TatI w'GTAC_w
Cuts at: 0 881 1233
Size: 881 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 684 716 1233
Size: 684 32 517
Fragments arranged by size:
684 517 32
Tth111I GACn'n_nGTC
Cuts at: 0 607 1233
Size: 607 626
XmnI GAAnn'nnTTC
Cuts at: 0 154 1233
Size: 154 1079
ZraI GAC'GTC
Cuts at: 0 494 1233
Size: 494 739
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI ApaI AvaI
BanI BanII BbsI BglII Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BstYI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MslI NheI NspI PasI
PciI PflMI PfoI PspOMI PstI SacI SalI SfcI
SmlI TaqII TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI Bpu10I BsaAI BsaBI BsiWI BsmI BspEI
BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI XbaI XcmI XhoI XmaI