(Circular) (Six-base) MAPSORT of: pFN21AE0711.seq Check: 3411 from: 1 to: 5974 pFN21AE0711 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2691 2691 Size: 5974 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3317 1937 Size: 976 404 4594 Fragments arranged by size: 4594 976 404 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 4037 5421 5689 1632 Size: 968 1437 1384 268 1917 Fragments arranged by size: 1917 1437 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2019 1166 Size: 853 5121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2770 3717 5289 2770 Size: 947 1572 3455 Fragments arranged by size: 3455 1572 947 ApaI G_GGCC'C Cuts at: 2250 3140 2250 Size: 890 5084 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2634 3299 1870 Size: 73 691 665 4545 Fragments arranged by size: 4545 691 665 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2290 2679 2760 3295 4750 Size: 325 217 627 503 389 81 535 1455 Cuts at: 4750 618 Size: 1842 Fragments arranged by size: 1842 1455 627 535 503 389 325 217 81 BanII G_rGCy'C Cuts at: 1391 2144 2250 2835 3140 3158 3293 1391 Size: 753 106 585 305 18 135 4072 Fragments arranged by size: 4072 753 585 305 135 106 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 2539 928 Size: 971 187 453 4363 Fragments arranged by size: 4363 971 453 187 BbvCI CC'TCA_GC Cuts at: 2717 2717 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2427 1548 Size: 879 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2639 3135 Size: 107 122 71 631 605 616 351 496 Cuts at: 3135 4585 136 Size: 1450 1525 Fragments arranged by size: 1525 1450 631 616 605 496 351 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 2606 3391 2606 Size: 785 5189 Bme1580I G_kGCm'C Cuts at: 1790 2250 2682 2735 3140 4035 5191 1790 Size: 460 432 53 405 895 1156 2573 Fragments arranged by size: 2573 1156 895 460 432 405 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3134 3705 4663 Size: 416 53 83 399 24 1717 571 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1753 1717 958 571 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2262 2327 2801 3154 4634 1239 Size: 830 193 65 474 353 1480 2579 Fragments arranged by size: 2579 1480 830 474 353 193 65 Bpu10I CC'TnA_GC Cuts at: 2058 2138 2690 2717 2058 Size: 80 552 27 5315 Fragments arranged by size: 5315 552 80 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2465 3247 4637 882 Size: 617 966 782 1390 2219 Fragments arranged by size: 2219 1390 966 782 617 BsaAI yAC'GTr Cuts at: 493 1204 2602 3765 493 Size: 711 1398 1163 2702 Fragments arranged by size: 2702 1398 1163 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2841 4407 4805 5079 5226 5637 2841 Size: 1566 398 274 147 411 3178 Fragments arranged by size: 3178 1566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3140 3152 3170 3182 1178 Size: 30 165 30 1737 12 18 12 3970 Fragments arranged by size: 3970 1737 165 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2152 2155 2191 2453 2660 3253 1186 Size: 966 3 36 262 207 593 3907 Fragments arranged by size: 3907 966 593 262 207 36 3 BseYI C'CCAG_C Cuts at: 2933 3112 5177 5673 2933 Size: 179 2065 496 3234 Fragments arranged by size: 3234 2065 496 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 2986 3251 1163 Size: 573 873 377 265 3886 Fragments arranged by size: 3886 873 573 377 265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2144 2696 3066 3158 4035 4120 5191 2064 Size: 80 552 370 92 877 85 1071 2847 Fragments arranged by size: 2847 1071 877 552 370 92 85 80 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2144 2250 2682 2696 2735 2835 Size: 399 274 80 106 432 14 39 100 Cuts at: 2835 3066 3140 3158 3293 4035 4120 5191 1391 Size: 231 74 18 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 274 231 135 106 100 85 80 74 39 18 14 BspEI T'CCGG_A Cuts at: 2841 5637 2841 Size: 2796 3178 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2445 2691 2784 3329 844 Size: 1601 246 93 545 3489 Fragments arranged by size: 3489 1601 545 246 93 BsrBI CCG'CTC Cuts at: 1394 1823 2988 3861 1394 Size: 429 1165 873 3507 Fragments arranged by size: 3507 1165 873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3129 4618 4805 1700 Size: 110 1319 1489 187 2869 Fragments arranged by size: 2869 1489 1319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 5974 BstYI r'GATC_y Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969 Size: 1332 367 385 17 1452 86 12 346 Cuts at: 5969 1972 Size: 1977 Fragments arranged by size: 1977 1452 1332 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2017 2105 2121 2619 2848 2898 2958 Size: 552 952 88 16 498 229 50 60 Cuts at: 2958 3016 513 Size: 58 3471 Fragments arranged by size: 3471 952 552 498 229 88 60 58 50 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2545 487 Size: 683 56 1319 3916 Fragments arranged by size: 3916 1319 683 56 BtsI GCAGTG_nn' Cuts at: 3182 3490 4284 4304 3182 Size: 308 794 20 4852 Fragments arranged by size: 4852 794 308 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2602 2602 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2409 2535 Size: 54 1425 50 275 18 193 386 126 Cuts at: 2535 2640 2895 2907 3421 4310 5939 8 Size: 105 255 12 514 889 1629 43 Fragments arranged by size: 1629 1425 889 514 386 275 255 193 126 105 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2269 2351 2516 3017 3904 1353 Size: 916 82 165 501 887 3423 Fragments arranged by size: 3423 916 887 501 165 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2968 3221 4561 4935 5081 387 Size: 1064 138 1379 253 1340 374 146 1280 Fragments arranged by size: 1379 1340 1280 1064 374 253 146 138 EcoICRI GAG'CTC Cuts at: 2142 3156 2142 Size: 1014 4960 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2262 2327 2600 2801 3154 4037 Size: 393 437 193 65 273 201 353 883 Cuts at: 4037 4634 5421 5689 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 883 787 597 437 393 353 273 268 201 193 65 EcoO109I rG'GnC_Cy Cuts at: 2235 2246 2247 3136 2235 Size: 11 1 889 5073 Fragments arranged by size: 5073 889 11 1 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2482 2804 3184 5121 1678 Size: 804 322 380 1937 2531 Fragments arranged by size: 2531 1937 804 380 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3140 3172 4615 4647 4689 Size: 32 135 32 1568 32 1443 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 1568 1443 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3318 3564 669 Size: 988 281 568 812 246 3079 Fragments arranged by size: 3079 988 812 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2406 5088 5272 1305 Size: 1101 2682 184 2007 Fragments arranged by size: 2682 2007 1101 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2025 2897 10 Size: 54 1425 343 193 872 3087 Fragments arranged by size: 3087 1425 872 343 193 54 MslI CAynn'nnrTG Cuts at: 518 1166 2019 2888 2903 3932 4291 4450 518 Size: 648 853 869 15 1029 359 159 2042 Fragments arranged by size: 2042 1029 869 853 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2029 2414 2630 2757 3390 4067 5215 5460 Size: 423 385 216 127 633 677 1148 245 Cuts at: 5460 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 633 423 385 245 216 127 NaeI GCC'GGC Cuts at: 3131 3131 Size: 5974 NcoI C'CATG_G Cuts at: 513 1065 2105 2121 2898 2958 3016 513 Size: 552 1040 16 777 60 58 3471 Fragments arranged by size: 3471 1040 777 552 60 58 16 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 3129 3129 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 3268 1348 Size: 1920 4054 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 3177 1108 Size: 2069 3905 PfoI T'CCnGG_A Cuts at: 3051 3051 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2602 2602 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2113 4043 4075 5556 5588 2081 Size: 32 1930 32 1481 32 2467 Fragments arranged by size: 2467 1930 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2235 2235 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2246 3136 2246 Size: 890 5084 PstI C_TGCA'G Cuts at: 830 2072 3326 830 Size: 1242 1254 3478 Fragments arranged by size: 3478 1254 1242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2029 2630 2029 Size: 601 5373 SacI G_AGCT'C Cuts at: 2144 3158 2144 Size: 1014 4960 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 3017 3017 Size: 5974 SbfI CC_TGCA'GG Cuts at: 2072 3326 2072 Size: 1254 4720 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2068 2722 3322 4457 5138 5329 826 Size: 219 1023 654 600 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 681 654 600 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3135 3135 Size: 5974 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 2636 3301 1872 Size: 764 665 4545 Fragments arranged by size: 4545 764 665 SmlI C'TyrA_G Cuts at: 820 1017 1943 4078 4979 5241 820 Size: 197 926 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 3194 1517 Size: 1677 4297 StyI C'CwwG_G Cuts at: 513 1065 2105 2121 2898 2958 3016 3413 513 Size: 552 1040 16 777 60 58 397 3074 Fragments arranged by size: 3074 1040 777 552 397 60 58 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2644 4001 4018 4171 4356 4801 325 Size: 1255 1064 1357 17 153 185 445 1498 Fragments arranged by size: 1498 1357 1255 1064 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2640 2672 2997 3029 5934 5966 2640 Size: 32 325 32 2905 32 2648 Fragments arranged by size: 2905 2648 325 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2890 2119 Size: 771 5203 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 2634 3299 1870 Size: 764 665 4545 Fragments arranged by size: 4545 764 665 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI