(Circular) (Six-base) MAPSORT of: pFN21AE0708.seq Check: 8065 from: 1 to: 5926 pFN21AE0708 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2243 2243 Size: 5926 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2500 2752 3989 5373 5641 1632 Size: 868 252 1237 1384 268 1917 Fragments arranged by size: 1917 1384 1237 868 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2810 4825 5802 1203 Size: 1607 2015 977 1327 Fragments arranged by size: 2015 1607 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 2280 2364 1166 Size: 1114 84 4728 Fragments arranged by size: 4728 1114 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 2356 3669 5241 2356 Size: 1313 1572 3041 Fragments arranged by size: 3041 1572 1313 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2069 2868 2912 3167 3235 3461 3613 5722 Size: 576 799 44 255 68 226 152 2109 Cuts at: 5722 1493 Size: 1697 Fragments arranged by size: 2109 1697 799 576 255 226 152 68 44 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 3251 1870 Size: 73 1308 4545 Fragments arranged by size: 4545 1308 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 3247 4702 618 Size: 325 217 627 407 1053 1455 1842 Fragments arranged by size: 1842 1455 1053 627 407 325 217 BanII G_rGCy'C Cuts at: 1391 3245 1391 Size: 1854 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2506 928 Size: 971 162 445 4348 Fragments arranged by size: 4348 971 445 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BfrBI ATG'CAT Cuts at: 2655 2655 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4537 136 Size: 107 122 71 631 605 337 2528 1525 Fragments arranged by size: 2528 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2042 3987 5143 1790 Size: 252 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 252 BmgBI CAC'GTC Cuts at: 1318 2059 1318 Size: 741 5185 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2589 3657 4615 Size: 416 53 83 399 24 1172 1068 958 Cuts at: 4615 442 Size: 1753 Fragments arranged by size: 1753 1172 1068 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2543 2898 2982 3082 3150 4586 1239 Size: 917 387 355 84 100 68 1436 2579 Fragments arranged by size: 2579 1436 917 387 355 100 84 68 Bpu10I CC'TnA_GC Cuts at: 2021 2543 2021 Size: 522 5404 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2811 3717 493 Size: 711 1607 906 2702 Fragments arranged by size: 2702 1607 906 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3733 4115 4750 275 Size: 53 83 186 936 2200 382 635 1451 Fragments arranged by size: 2200 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4621 Fragments arranged by size: 4621 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 1186 Size: 1288 4638 BseYI C'CCAG_C Cuts at: 5129 5625 5129 Size: 496 5430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 3085 3169 1163 Size: 573 606 743 84 3920 Fragments arranged by size: 3920 743 606 573 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3376 4137 5165 1540 Size: 275 186 15 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2427 3987 4072 5143 2427 Size: 1560 85 1071 3210 Fragments arranged by size: 3210 1560 1071 85 BsmI GAATG_Cn' Cuts at: 2984 3437 3530 2984 Size: 453 93 5380 Fragments arranged by size: 5380 453 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2427 3245 3987 4072 5143 1391 Size: 399 252 385 818 742 85 1071 2174 Fragments arranged by size: 2174 1071 818 742 399 385 252 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2243 3281 844 Size: 1399 1038 3489 Fragments arranged by size: 3489 1399 1038 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2937 4421 4595 65 Size: 1183 1689 1484 174 1396 Fragments arranged by size: 1689 1484 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 2857 96 Size: 2761 3165 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 2693 3986 4998 2693 Size: 1293 1012 3621 Fragments arranged by size: 3621 1293 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 794 Size: 1562 4364 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921 Size: 1284 367 385 17 1452 86 12 346 Cuts at: 5921 1972 Size: 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4435 Fragments arranged by size: 4435 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 2989 3073 3157 3442 4236 4256 2989 Size: 84 84 285 794 20 4659 Fragments arranged by size: 4659 794 285 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 3373 4262 Size: 54 1425 50 275 18 183 1360 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1360 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 3373 1537 Size: 275 201 1360 4090 Fragments arranged by size: 4090 1360 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 3187 3856 1353 Size: 1118 716 669 3423 Fragments arranged by size: 3423 1118 716 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2500 2543 2752 2898 2982 3082 Size: 393 524 344 43 209 146 84 100 Cuts at: 3082 3150 3989 4586 5373 5641 1239 Size: 68 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 597 524 393 344 268 209 146 100 84 68 43 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2250 2099 Size: 151 5775 EcoRI G'AATT_C Cuts at: 2868 3235 3613 2868 Size: 367 378 5181 Fragments arranged by size: 5181 378 367 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 5073 1678 Size: 3395 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 4567 4599 4641 Size: 32 135 32 1022 32 1941 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 1941 1022 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 2364 2418 2893 2977 3884 4243 Size: 648 1114 84 54 475 84 907 359 Cuts at: 4243 4402 518 Size: 159 2042 Fragments arranged by size: 2042 1114 907 648 475 359 159 84 84 54 MspA1I CmG'CkG Cuts at: 1606 2643 3342 4019 5167 5412 1606 Size: 1037 699 677 1148 245 2120 Fragments arranged by size: 2120 1148 1037 699 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4435 Fragments arranged by size: 4435 939 552 NdeI CA'TA_TG Cuts at: 387 2890 2974 387 Size: 2503 84 3339 Fragments arranged by size: 3339 2503 84 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 2013 3373 2013 Size: 1360 4566 NsiI A_TGCA'T Cuts at: 2657 2657 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2321 2495 3284 4829 1132 Size: 97 1092 174 789 1545 2229 Fragments arranged by size: 2229 1545 1092 789 174 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 5113 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2234 1613 Size: 621 5305 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 3278 830 Size: 2448 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2643 2643 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2443 2709 3274 4409 5090 5281 826 Size: 219 1398 266 565 1135 681 191 1471 Fragments arranged by size: 1471 1398 1135 681 565 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2372 1517 Size: 855 5071 StyI C'CwwG_G Cuts at: 513 1065 2004 3365 513 Size: 552 939 1361 3074 Fragments arranged by size: 3074 1361 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3953 3970 4123 4308 4753 325 Size: 1255 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2857 4172 5884 Size: 274 80 33 51 1443 880 1315 1712 Cuts at: 5884 96 Size: 138 Fragments arranged by size: 1712 1443 1315 880 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2642 2642 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI