(Circular) (Six-base) MAPSORT of: pFN21AE4132.seq Check: 920 from: 1 to: 6304 pFN21AE4132 6304 bp With 182 enzymes: SgfI * March 28, 2012 13:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2316 3070 3263 2316 Size: 754 193 5357 Fragments arranged by size: 5357 754 193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3220 3625 3220 Size: 405 5899 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4367 5751 6019 1632 Size: 2735 1384 268 1917 Fragments arranged by size: 2735 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 2084 5033 2084 Size: 2949 3355 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2781 2817 1184 Size: 1597 36 4671 Fragments arranged by size: 4671 1597 36 AlwNI CAG_nnn'CTG Cuts at: 2074 3062 4047 5619 2074 Size: 988 985 1572 2759 Fragments arranged by size: 2759 1572 988 985 ApaI G_GGCC'C Cuts at: 2710 2710 Size: 6304 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 3613 3839 3991 6100 1493 Size: 2120 226 152 2109 1697 Fragments arranged by size: 2120 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2447 2691 2709 3225 3413 3513 3629 Size: 73 504 244 18 516 188 100 116 Cuts at: 3629 1870 Size: 4545 Fragments arranged by size: 4545 516 504 244 188 116 100 73 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2028 2061 2028 Size: 33 6271 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3220 3349 3625 5080 618 Size: 325 217 627 1433 129 276 1455 1842 Fragments arranged by size: 1842 1455 1433 627 325 276 217 129 BanII G_rGCy'C Cuts at: 1391 2171 2370 2388 2661 2710 2895 3042 3212 Size: 780 199 18 273 49 185 147 170 Cuts at: 3212 3623 1391 Size: 411 4072 Fragments arranged by size: 4072 780 411 273 199 185 170 147 49 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2998 928 Size: 971 1099 4234 Fragments arranged by size: 4234 1099 971 BbvCI CC'TCA_GC Cuts at: 2213 2213 Size: 6304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3471 3505 4495 4529 1903 Size: 34 50 34 1450 34 990 34 3678 Fragments arranged by size: 3678 1450 990 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 2417 4915 Size: 107 122 71 631 605 688 57 2498 Cuts at: 4915 136 Size: 1525 Fragments arranged by size: 2498 1525 688 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 2563 6299 2563 Size: 3736 2568 BlpI GC'TnA_GC Cuts at: 2364 3302 3721 2364 Size: 938 419 4947 Fragments arranged by size: 4947 938 419 Bme1580I G_kGCm'C Cuts at: 1790 2039 2635 2710 4365 5521 1790 Size: 249 596 75 1655 1156 2573 Fragments arranged by size: 2573 1655 1156 596 249 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2889 2917 4035 Size: 416 53 83 399 24 1472 28 1118 Cuts at: 4035 4993 442 Size: 958 1753 Fragments arranged by size: 1753 1472 1118 958 416 399 83 53 28 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2296 3070 4964 1239 Size: 1057 774 1894 2579 Fragments arranged by size: 2579 1894 1057 774 Bpu10I CC'TnA_GC Cuts at: 2012 2213 3036 2012 Size: 201 823 5280 Fragments arranged by size: 5280 823 201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3206 4429 5294 5592 3206 Size: 1223 865 298 3918 Fragments arranged by size: 3918 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2444 3017 4967 882 Size: 617 945 573 1950 2219 Fragments arranged by size: 2219 1950 945 617 573 BsaAI yAC'GTr Cuts at: 493 1204 3121 4095 493 Size: 711 1917 974 2702 Fragments arranged by size: 2702 1917 974 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4111 4493 5128 275 Size: 53 83 186 936 2578 382 635 1451 Fragments arranged by size: 2578 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2102 4737 5135 5409 5556 5967 2102 Size: 2635 398 274 147 411 2439 Fragments arranged by size: 2635 2439 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2843 2873 3098 3128 1178 Size: 30 165 30 1440 30 225 30 4354 Fragments arranged by size: 4354 1440 225 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2288 2414 2927 1186 Size: 886 216 126 513 4563 Fragments arranged by size: 4563 886 513 216 126 BseYI C'CCAG_C Cuts at: 3280 3599 5507 6003 3280 Size: 319 1908 496 3581 Fragments arranged by size: 3581 1908 496 319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2406 3048 3575 1163 Size: 573 670 642 527 3892 Fragments arranged by size: 3892 670 642 573 527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2171 2370 2388 3042 3414 4365 4450 5521 2171 Size: 199 18 654 372 951 85 1071 2954 Fragments arranged by size: 2954 1071 951 654 372 199 85 18 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 BsmBI CGTCTCn'nnnn_ Cuts at: 2092 2605 2092 Size: 513 5791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2171 2370 2388 2635 2661 2710 Size: 399 249 132 199 18 247 26 49 Cuts at: 2710 2895 3042 3212 3414 3623 4365 4450 5521 Size: 185 147 170 202 209 742 85 1071 Cuts at: 5521 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 249 247 209 202 199 185 170 147 132 85 49 26 18 BspEI T'CCGG_A Cuts at: 2102 5967 2102 Size: 3865 2439 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2082 2316 3070 3263 3306 3604 3659 844 Size: 1238 234 754 193 43 298 55 3489 Fragments arranged by size: 3489 1238 754 298 234 193 55 43 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3126 4191 1394 Size: 429 193 1110 1065 3507 Fragments arranged by size: 3507 1110 1065 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstXI CCAn_nnnn'nTGG Cuts at: 2869 2869 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2563 3634 4001 4386 4403 5855 5941 5953 Size: 591 1071 367 385 17 1452 86 12 Cuts at: 5953 6299 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1071 591 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2244 513 Size: 552 1179 4573 Fragments arranged by size: 4573 1179 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2497 487 Size: 683 56 1271 4294 Fragments arranged by size: 4294 1271 683 56 BtsI GCAGTG_nn' Cuts at: 2703 3542 3820 4614 4634 2703 Size: 839 278 794 20 4373 Fragments arranged by size: 4373 839 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2458 5311 809 Size: 507 1142 2853 1802 Fragments arranged by size: 2853 1802 1142 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 2497 3145 Size: 54 1425 50 275 18 293 374 648 Cuts at: 3145 3232 3751 4640 6269 8 Size: 87 519 889 1629 43 Fragments arranged by size: 1629 1425 889 648 519 374 293 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 4234 1353 Size: 823 2058 3423 Fragments arranged by size: 3423 2058 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2264 4891 5265 5411 387 Size: 1064 138 675 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 675 374 146 138 EcoICRI GAG'CTC Cuts at: 2169 2368 2386 3040 2169 Size: 199 18 654 5433 Fragments arranged by size: 5433 654 199 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2296 3070 4367 4964 5751 6019 1239 Size: 393 664 774 1297 597 787 268 1524 Fragments arranged by size: 1524 1297 787 774 664 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2071 2209 2473 3295 2071 Size: 138 264 822 5080 Fragments arranged by size: 5080 822 264 138 EcoRI G'AATT_C Cuts at: 3613 3991 3613 Size: 378 5926 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2548 2580 4945 Size: 32 135 32 498 32 446 32 2365 Cuts at: 4945 4977 5019 5051 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2365 498 446 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 3304 748 Size: 2556 3748 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3224 3629 3224 Size: 405 5899 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 2886 5418 5602 1305 Size: 939 642 2532 184 2007 Fragments arranged by size: 2532 2007 939 642 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2125 3147 3234 10 Size: 54 1425 343 293 1022 87 3080 Fragments arranged by size: 3080 1425 1022 343 293 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 3000 4262 4621 4780 518 Size: 648 1834 1262 359 159 2042 Fragments arranged by size: 2042 1834 1262 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2351 2578 2721 2761 3095 3720 4397 5545 Size: 745 227 143 40 334 625 677 1148 Cuts at: 5545 5790 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 745 677 625 334 245 227 143 40 NcoI C'CATG_G Cuts at: 513 1065 2244 513 Size: 552 1179 4573 Fragments arranged by size: 4573 1179 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 2506 3662 5207 1132 Size: 97 1277 1156 1545 2229 Fragments arranged by size: 2229 1545 1277 1156 97 PasI CC'CwG_GG Cuts at: 1348 2032 2258 2983 1348 Size: 684 226 725 4669 Fragments arranged by size: 4669 725 684 226 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2581 3154 3241 1108 Size: 1473 573 87 4171 Fragments arranged by size: 4171 1473 573 87 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 3295 2071 Size: 1224 5080 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2706 2706 Size: 6304 PstI C_TGCA'G Cuts at: 830 2160 3396 3656 830 Size: 1330 1236 260 3478 Fragments arranged by size: 3478 1330 1236 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2351 2578 2761 3095 2351 Size: 227 183 334 5560 Fragments arranged by size: 5560 334 227 183 SacI G_AGCT'C Cuts at: 2171 2370 2388 3042 2171 Size: 199 18 654 5433 Fragments arranged by size: 5433 654 199 18 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SapI GCTCTTCn'nnn_ Cuts at: 2176 2176 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2156 2316 2851 3392 3421 3652 4787 Size: 219 1111 160 535 541 29 231 1135 Cuts at: 4787 5468 5659 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 541 535 231 219 191 160 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 2711 3631 1872 Size: 839 920 4545 Fragments arranged by size: 4545 920 839 SmlI C'TyrA_G Cuts at: 820 1017 1943 3185 4408 5309 5571 820 Size: 197 926 1242 1223 901 262 1553 Fragments arranged by size: 1553 1242 1223 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SrfI GCCC'GGGC Cuts at: 2711 2711 Size: 6304 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2411 3314 3511 1517 Size: 894 903 197 4310 Fragments arranged by size: 4310 903 894 197 StyI C'CwwG_G Cuts at: 513 1065 2244 2469 2952 3506 3743 513 Size: 552 1179 225 483 554 237 3074 Fragments arranged by size: 3074 1179 554 552 483 237 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 2318 2975 3242 3272 4331 4348 Size: 1255 501 237 657 267 30 1059 17 Cuts at: 4348 4501 4686 5131 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1059 657 501 445 267 237 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4550 6262 96 Size: 274 80 33 51 1443 2573 1712 138 Fragments arranged by size: 2573 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6264 6296 6264 Size: 32 6272 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 2709 3629 1870 Size: 839 920 4545 Fragments arranged by size: 4545 920 839 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI XcmI