(Linear) (Six-base) MAPSORT of: KIEE4132.seq Check: 8373 from: 1 to: 1599 KIEE4132.orf With 182 enzymes: SgfI * March 22, 2012 17:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 311 1065 1258 1599 Size: 311 754 193 341 Fragments arranged by size: 754 341 311 193 Acc65I G'GTAC_C Cuts at: 0 1215 1599 Size: 1215 384 AhdI GACnn_n'nnGTC Cuts at: 0 79 1599 Size: 79 1520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 776 812 1599 Size: 776 36 787 Fragments arranged by size: 787 776 36 AlwNI CAG_nnn'CTG Cuts at: 0 69 1057 1599 Size: 69 988 542 Fragments arranged by size: 988 542 69 ApaI G_GGCC'C Cuts at: 0 705 1599 Size: 705 894 AvaI C'yCGr_G Cuts at: 0 442 686 704 1220 1408 1508 1599 Size: 442 244 18 516 188 100 91 Fragments arranged by size: 516 442 244 188 100 91 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 23 56 1599 Size: 23 33 1543 Fragments arranged by size: 1543 33 23 BanI G'GyrC_C Cuts at: 0 1215 1344 1599 Size: 1215 129 255 Fragments arranged by size: 1215 255 129 BanII G_rGCy'C Cuts at: 0 166 365 383 656 705 890 1037 1207 Size: 166 199 18 273 49 185 147 170 Cuts at: 1207 1599 Size: 392 Fragments arranged by size: 392 273 199 185 170 166 147 49 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 993 1599 Size: 993 606 BbvCI CC'TCA_GC Cuts at: 0 208 1599 Size: 208 1391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1466 1500 1599 Size: 1466 34 99 Fragments arranged by size: 1466 99 34 BglI GCCn_nnn'nGGC Cuts at: 0 355 412 1599 Size: 355 57 1187 Fragments arranged by size: 1187 355 57 BglII A'GATC_T Cuts at: 0 558 1599 Size: 558 1041 BlpI GC'TnA_GC Cuts at: 0 359 1297 1599 Size: 359 938 302 Fragments arranged by size: 938 359 302 Bme1580I G_kGCm'C Cuts at: 0 34 630 705 1599 Size: 34 596 75 894 Fragments arranged by size: 894 596 75 34 BmrI ACTGGGnnnn_n' Cuts at: 0 884 912 1599 Size: 884 28 687 Fragments arranged by size: 884 687 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 291 1065 1599 Size: 291 774 534 Fragments arranged by size: 774 534 291 Bpu10I CC'TnA_GC Cuts at: 0 7 208 1031 1599 Size: 7 201 823 568 Fragments arranged by size: 823 568 201 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1201 1599 Size: 1201 398 BsaI GGTCTCn'nnnn_ Cuts at: 0 439 1012 1599 Size: 439 573 587 Fragments arranged by size: 587 573 439 BsaAI yAC'GTr Cuts at: 0 1116 1599 Size: 1116 483 BsaWI w'CCGG_w Cuts at: 0 97 1599 Size: 97 1502 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 838 868 1093 1123 1599 Size: 838 30 225 30 476 Fragments arranged by size: 838 476 225 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 283 409 922 1599 Size: 67 216 126 513 677 Fragments arranged by size: 677 513 216 126 67 BseYI C'CCAG_C Cuts at: 0 1275 1594 1599 Size: 1275 319 5 Fragments arranged by size: 1275 319 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 1043 1570 1599 Size: 401 642 527 29 Fragments arranged by size: 642 527 401 29 BsiHKAI G_wGCw'C Cuts at: 0 166 365 383 1037 1409 1599 Size: 166 199 18 654 372 190 Fragments arranged by size: 654 372 199 190 166 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 87 600 1599 Size: 87 513 999 Fragments arranged by size: 999 513 87 Bsp1286I G_dGCh'C Cuts at: 0 34 166 365 383 630 656 705 890 Size: 34 132 199 18 247 26 49 185 Cuts at: 890 1037 1207 1409 1599 Size: 147 170 202 190 Fragments arranged by size: 247 202 199 190 185 170 147 132 49 34 26 18 BspEI T'CCGG_A Cuts at: 0 97 1599 Size: 97 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 77 311 1065 1258 1301 1599 Size: 77 234 754 193 43 298 Fragments arranged by size: 754 298 234 193 77 43 BsrBI CCG'CTC Cuts at: 0 11 1121 1599 Size: 11 1110 478 Fragments arranged by size: 1110 478 11 BstXI CCAn_nnnn'nTGG Cuts at: 0 864 1599 Size: 864 735 BstYI r'GATC_y Cuts at: 0 558 1599 Size: 558 1041 BtgI C'CryG_G Cuts at: 0 239 1599 Size: 239 1360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 492 1599 Size: 492 1107 BtsI GCAGTG_nn' Cuts at: 0 698 1537 1599 Size: 698 839 62 Fragments arranged by size: 839 698 62 DrdI GACnn_nn'nnGTC Cuts at: 0 453 1599 Size: 453 1146 EaeI y'GGCC_r Cuts at: 0 118 492 1140 1227 1599 Size: 118 374 648 87 372 Fragments arranged by size: 648 374 372 118 87 EarI CTCTTCn'nnn_ Cuts at: 0 171 1599 Size: 171 1428 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 259 1599 Size: 259 1340 EcoICRI GAG'CTC Cuts at: 0 164 363 381 1035 1599 Size: 164 199 18 654 564 Fragments arranged by size: 654 564 199 164 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 291 1065 1599 Size: 291 774 534 Fragments arranged by size: 774 534 291 EcoO109I rG'GnC_Cy Cuts at: 0 66 204 468 1290 1599 Size: 66 138 264 822 309 Fragments arranged by size: 822 309 264 138 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 65 97 543 575 1599 Size: 65 32 446 32 1024 Fragments arranged by size: 1024 446 65 32 32 HindIII A'AGCT_T Cuts at: 0 1299 1599 Size: 1299 300 KpnI G_GTAC'C Cuts at: 0 1219 1599 Size: 1219 380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 881 1599 Size: 239 642 718 Fragments arranged by size: 718 642 239 MscI TGG'CCA Cuts at: 0 120 1142 1229 1599 Size: 120 1022 87 370 Fragments arranged by size: 1022 370 120 87 MslI CAynn'nnrTG Cuts at: 0 995 1599 Size: 995 604 MspA1I CmG'CkG Cuts at: 0 346 573 716 756 1090 1599 Size: 346 227 143 40 334 509 Fragments arranged by size: 509 346 334 227 143 40 NcoI C'CATG_G Cuts at: 0 239 1599 Size: 239 1360 NspI r_CATG'y Cuts at: 0 501 1599 Size: 501 1098 PasI CC'CwG_GG Cuts at: 0 27 253 978 1599 Size: 27 226 725 621 Fragments arranged by size: 725 621 226 27 PflMI CCAn_nnn'nTGG Cuts at: 0 576 1149 1236 1599 Size: 576 573 87 363 Fragments arranged by size: 576 573 363 87 PfoI T'CCnGG_A Cuts at: 0 365 1599 Size: 365 1234 PpuMI rG'GwC_Cy Cuts at: 0 66 1290 1599 Size: 66 1224 309 Fragments arranged by size: 1224 309 66 PspOMI G'GGCC_C Cuts at: 0 701 1599 Size: 701 898 PstI C_TGCA'G Cuts at: 0 155 1391 1599 Size: 155 1236 208 Fragments arranged by size: 1236 208 155 PvuII CAG'CTG Cuts at: 0 346 573 756 1090 1599 Size: 346 227 183 334 509 Fragments arranged by size: 509 346 334 227 183 SacI G_AGCT'C Cuts at: 0 166 365 383 1037 1599 Size: 166 199 18 654 562 Fragments arranged by size: 654 562 199 166 18 SapI GCTCTTCn'nnn_ Cuts at: 0 171 1599 Size: 171 1428 SfcI C'TryA_G Cuts at: 0 151 311 846 1387 1416 1599 Size: 151 160 535 541 29 183 Fragments arranged by size: 541 535 183 160 151 29 SmaI CCC'GGG Cuts at: 0 706 1599 Size: 706 893 SmlI C'TyrA_G Cuts at: 0 1180 1599 Size: 1180 419 SrfI GCCC'GGGC Cuts at: 0 706 1599 Size: 706 893 StuI AGG'CCT Cuts at: 0 406 1309 1506 1599 Size: 406 903 197 93 Fragments arranged by size: 903 406 197 93 StyI C'CwwG_G Cuts at: 0 239 464 947 1501 1599 Size: 239 225 483 554 98 Fragments arranged by size: 554 483 239 225 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 76 313 970 1237 1267 1599 Size: 76 237 657 267 30 332 Fragments arranged by size: 657 332 267 237 76 30 XmaI C'CCGG_G Cuts at: 0 704 1599 Size: 704 895 Enzymes that do cut: AarI Acc65I AhdI AlfI AlwNI ApaI AvaI BaeI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI SmaI SmlI SrfI StuI StyI TaqII XmaI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AflIII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI