Restriction map for
pFN21AE0703
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# NO CUT ENZYMES ( 176 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 606 GACGTC GACGT^C
AccI 609 GTCTAC GT^[AC][GT]AC
AciI 46 CCGC CCGC(-3/-1)
AcyI 606 GACGTC G[GA]^CG[CT]C
ApaI 693 GGGCCC GGGCC^C
BanII 693 GGGCCC G[GA]GC[CT]^C
BbsI 675 GAAGAC GAAGAC(2/6)
BcnI 18 CCCGG CC^[GC]GG
BglII 179 AGATCT A^GATCT
BmyI 693 GGGCCC G[AGT]GC[ACT]^C
BpmI 131 CTGGAG CTGGAG(16/14)
BpuAI 675 GAAGAC GAAGAC(2/6)
BsaAI 629 TACGTA [CT]AC^GT[GA]
BsaHI 606 GACGTC G[GA]^CG[CT]C
BsaJI 690 CCAGGG C^C[ACGT][ACGT]GG
BseRI 484 GAGGAG GAGGAG(10/8)
BsmAI 826 GAGAC GTCTC(1/5)
Bsp1286I 693 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 709 TGTACA T^GTACA
BspMI 81 GCAGGT ACCTGC(4/8)
BsrGI 709 TGTACA T^GTACA
BstYI 179 AGATCT [GA]^GATC[CT]
Cac8I 89 GCAAGC GC[ACGT]^[ACGT]GC
DpnII 180 GATC ^GATC
EcoT22I 263 ATGCAT ATGCA^T
HapII 19 CCGG C^CGG
Hin1I 606 GACGTC G[GA]^CG[CT]C
HpaII 19 CCGG C^CGG
HphI 334 GGTGA GGTGA(8/7)
MaeIII 211 GTGAC ^GT[ACGT]AC
MboI 180 GATC ^GATC
MflI 179 AGATCT [GA]^GATC[CT]
MspI 19 CCGG C^CGG
MwoI 39 GCTTAATCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 18 CCCGG CC^[GC]GG
NdeII 180 GATC ^GATC
NsiI 263 ATGCAT ATGCA^T
Sau3AI 180 GATC ^GATC
SmlI 740 CTCAAG C^T[CT][GA]AG
SnaBI 629 TACGTA TAC^GTA
SspBI 709 TGTACA T^GTACA
SspI 403 AATATT AAT^ATT
TaqI 59 TCGA T^CGA
TfiI 453 GAATC G^A[AT]TC
Tsp45I 211 GTGAC ^GT[GC]AC
TthHB8I 59 TCGA T^CGA
XhoII 179 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 473 AGCT AG^CT
528 AGCT
BbvI 417 GCAGC GCAGC(8/12)
869 GCAGC
BstF5I 288 GGATG GGATG(2/0)
479 GGATG
DraI 391 TTTAAA TTT^AAA
493 TTTAAA
DraII 692 AGGGCCC [GA]G^G[ACGT]CC[CT]
693 GGGCCCC
EcoO109I 692 AGGGCCC [GA]G^G[ACGT]CC[CT]
693 GGGCCCC
Fnu4HI 417 GCAGC GC^[ACGT]GC
869 GCAGC
FokI 288 GGATG GGATG(9/13)
479 GGATG
ItaI 417 GCAGC GC^[ACGT]GC
869 GCAGC
MaeII 607 ACGT A^CGT
630 ACGT
NlaIV 693 GGGCCC GG[ACGT]^[ACGT]CC
694 GGCCCC
TseI 417 GCAGC G^C[AT]GC
869 GCAGC
Tsp509I 524 AATT ^AATT
540 AATT
TspRI 207 CACTG CA[GC]TG[ACGT][ACGT]^
434 CAGTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI BsrI BssKI BstNI CfoI
Cfr13I DdeI EcoRII HaeIII HhaI
HinP1I HinfI MboII MnlI MseI
MvaI PleI RsaI Sau96I ScrFI
Tru9I