(Circular) (Six-base) MAPSORT of: pFN21AE4117.seq Check: 8100 from: 1 to: 6187 pFN21AE4117 6187 bp With 182 enzymes: SgfI * March 28, 2012 13:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 2753 2846 3256 3474 4250 5634 5902 Size: 932 189 93 410 218 776 1384 268 Cuts at: 5902 1632 Size: 1917 Fragments arranged by size: 1917 1384 932 776 410 268 218 189 93 AflII C'TTAA_G Cuts at: 820 1017 2258 820 Size: 197 1241 4749 Fragments arranged by size: 4749 1241 197 AflIII A'CryG_T Cuts at: 1203 3139 5086 6063 1203 Size: 1936 1947 977 1327 Fragments arranged by size: 1947 1936 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 3350 3930 5502 3350 Size: 580 1572 4035 Fragments arranged by size: 4035 1572 580 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2513 3496 3722 3874 5983 1493 Size: 1020 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 1020 983 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 BamHI G'GATC_C Cuts at: 2975 3517 3884 2975 Size: 542 367 5278 Fragments arranged by size: 5278 542 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3508 4963 618 Size: 325 217 627 248 1473 1455 1842 Fragments arranged by size: 1842 1473 1455 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 3259 3506 1391 Size: 1868 247 4072 Fragments arranged by size: 4072 1868 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 2235 3604 2235 Size: 1369 4818 Bme1580I G_kGCm'C Cuts at: 1790 4248 5404 1790 Size: 2458 1156 2573 Fragments arranged by size: 2573 2458 1156 BmgBI CAC'GTC Cuts at: 1318 2827 1318 Size: 1509 4678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2672 3327 3399 4847 1239 Size: 1433 655 72 1448 2579 Fragments arranged by size: 2579 1448 1433 655 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 4312 5177 5475 2300 Size: 2012 865 298 3012 Fragments arranged by size: 3012 2012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 3089 4850 882 Size: 617 808 782 1761 2219 Fragments arranged by size: 2219 1761 808 782 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2298 4620 5018 5292 5439 5850 2298 Size: 2322 398 274 147 411 2635 Fragments arranged by size: 2635 2322 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5962 Fragments arranged by size: 5962 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2703 3107 1186 Size: 1517 404 4266 Fragments arranged by size: 4266 1517 404 BseYI C'CCAG_C Cuts at: 2281 3099 5390 5886 2281 Size: 818 2291 496 2582 Fragments arranged by size: 2582 2291 818 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5614 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 3637 4398 5426 1540 Size: 275 186 55 1581 761 1028 2301 Fragments arranged by size: 2301 1581 1028 761 275 186 55 BsiHKAI G_wGCw'C Cuts at: 4248 4333 5404 4248 Size: 85 1071 5031 Fragments arranged by size: 5031 1071 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3259 3506 4248 4333 5404 1391 Size: 399 1469 247 742 85 1071 2174 Fragments arranged by size: 2174 1469 1071 742 399 247 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 3145 4076 5806 3145 Size: 931 1730 3526 Fragments arranged by size: 3526 1730 931 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 2367 4682 4856 65 Size: 1183 847 272 2315 174 1396 Fragments arranged by size: 2315 1396 1183 847 272 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2670 4831 5018 1700 Size: 110 860 2161 187 2869 Fragments arranged by size: 2869 2161 860 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2975 3517 3884 4269 4286 5738 5824 5836 Size: 1003 542 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1003 542 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 2847 3703 4497 4517 2847 Size: 856 794 20 4517 Fragments arranged by size: 4517 856 794 20 ClaI AT'CG_AT Cuts at: 1554 2211 1554 Size: 657 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DraIII CAC_nnn'GTG Cuts at: 2024 2024 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2834 3354 Size: 54 1425 50 275 18 223 781 520 Cuts at: 3354 3634 4523 6152 8 Size: 280 889 1629 43 Fragments arranged by size: 1629 1425 889 781 520 280 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2053 3634 1537 Size: 275 241 1581 4090 Fragments arranged by size: 4090 1581 275 241 EarI CTCTTCn'nnn_ Cuts at: 1353 2702 3108 4117 1353 Size: 1349 406 1009 3423 Fragments arranged by size: 3423 1349 1009 406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2672 2753 2846 3256 3327 3399 Size: 393 932 108 81 93 410 71 72 Cuts at: 3399 3474 4250 4847 5634 5902 1239 Size: 75 776 597 787 268 1524 Fragments arranged by size: 1524 932 787 776 597 410 393 268 108 93 81 75 72 71 EcoO109I rG'GnC_Cy Cuts at: 2346 3295 2346 Size: 949 5238 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2273 2273 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2380 2412 2489 2521 4828 Size: 32 135 32 808 32 77 32 2307 Cuts at: 4828 4860 4902 4934 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2307 808 135 77 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 2476 5301 5485 1305 Size: 871 300 2825 184 2007 Fragments arranged by size: 2825 2007 871 300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2836 3356 10 Size: 54 1425 343 1004 520 2841 Fragments arranged by size: 2841 1425 1004 520 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 2595 3144 4145 4504 4663 518 Size: 648 1357 72 549 1001 359 159 2042 Fragments arranged by size: 2042 1357 1001 648 549 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2239 3043 3419 3603 4280 5428 5673 1606 Size: 633 804 376 184 677 1148 245 2120 Fragments arranged by size: 2120 1148 804 677 633 376 245 184 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4696 Fragments arranged by size: 4696 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 3545 5090 1132 Size: 97 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3244 3276 4256 4288 5769 5801 3244 Size: 32 980 32 1481 32 3630 Fragments arranged by size: 3630 1481 980 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2346 3295 2346 Size: 949 5238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 2075 3539 830 Size: 1245 1464 3478 Fragments arranged by size: 3478 1464 1245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2239 3043 3419 2239 Size: 804 376 5007 Fragments arranged by size: 5007 804 376 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 2346 2346 Size: 6187 SapI GCTCTTCn'nnn_ Cuts at: 2702 2702 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2071 3535 4670 5351 5542 826 Size: 219 1026 1464 1135 681 191 1471 Fragments arranged by size: 1471 1464 1135 1026 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 2315 4291 5192 5454 820 Size: 197 926 315 57 1976 901 262 1553 Fragments arranged by size: 1976 1553 926 901 315 262 197 57 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 3626 513 Size: 552 939 1622 3074 Fragments arranged by size: 3074 1622 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4231 4384 4569 5014 325 Size: 1255 2634 17 153 185 445 1498 Fragments arranged by size: 2634 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4433 6145 96 Size: 274 80 33 51 1443 2456 1712 138 Fragments arranged by size: 2456 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2811 2843 6147 6179 2811 Size: 32 3304 32 2819 Fragments arranged by size: 3304 2819 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI