(Circular) (Six-base) MAPSORT of: pFN21AE0701.seq Check: 2979 from: 1 to: 5566 pFN21AE0701 5566 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2236 2236 Size: 5566 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2361 2909 1937 Size: 424 548 4594 Fragments arranged by size: 4594 548 424 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 2236 3693 4066 1563 Size: 673 1457 373 3063 Fragments arranged by size: 3063 1457 673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2829 3629 5013 5281 1632 Size: 1197 800 1384 268 1917 Fragments arranged by size: 1917 1384 1197 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5369 AflIII A'CryG_T Cuts at: 1203 4465 5442 1203 Size: 3262 977 1327 Fragments arranged by size: 3262 1327 977 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 2839 4295 2839 Size: 1456 4110 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5566 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 2045 Size: 32 5534 AlwNI CAG_nnn'CTG Cuts at: 2830 3309 4881 2830 Size: 479 1572 3515 Fragments arranged by size: 3515 1572 479 ApaLI G'TGCA_C Cuts at: 3623 4779 3623 Size: 1156 4410 ApoI r'AATT_y Cuts at: 1493 2156 2345 2432 2875 3101 3253 5362 1493 Size: 663 189 87 443 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 443 226 189 152 87 AseI AT'TA_AT Cuts at: 160 1032 2199 4120 160 Size: 872 1167 1921 1606 Fragments arranged by size: 1921 1606 1167 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2891 1870 Size: 73 948 4545 Fragments arranged by size: 4545 948 73 BamHI G'GATC_C Cuts at: 2130 2896 3263 2130 Size: 766 367 4433 Fragments arranged by size: 4433 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2887 4342 618 Size: 325 217 627 1100 1455 1842 Fragments arranged by size: 1842 1455 1100 627 325 217 BanII G_rGCy'C Cuts at: 1391 2464 2885 1391 Size: 1073 421 4072 Fragments arranged by size: 4072 1073 421 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2736 2835 928 Size: 971 837 99 3659 Fragments arranged by size: 3659 971 837 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3757 3791 1903 Size: 34 1820 34 3678 Fragments arranged by size: 3678 1820 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 1548 Size: 5566 BfrBI ATG'CAT Cuts at: 2723 2723 Size: 5566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2509 4177 Size: 107 122 71 631 605 337 500 1668 Cuts at: 4177 136 Size: 1525 Fragments arranged by size: 1668 1525 631 605 500 337 122 107 71 BglII A'GATC_T Cuts at: 5561 5561 Size: 5566 BlpI GC'TnA_GC Cuts at: 2513 2983 2513 Size: 470 5096 Bme1580I G_kGCm'C Cuts at: 1790 3627 4783 1790 Size: 1837 1156 2573 Fragments arranged by size: 2573 1837 1156 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 4561 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2136 2501 2662 Size: 416 53 83 399 24 719 365 161 Cuts at: 2662 3297 4255 442 Size: 635 958 1753 Fragments arranged by size: 1753 958 719 635 416 399 365 161 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 4226 1239 Size: 808 2179 2579 Fragments arranged by size: 2579 2179 808 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3691 4556 4854 3691 Size: 865 298 4403 Fragments arranged by size: 4403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 4229 882 Size: 617 582 2148 2219 Fragments arranged by size: 2219 2148 617 582 BsaAI yAC'GTr Cuts at: 493 1204 3357 493 Size: 711 2153 2702 Fragments arranged by size: 2702 2153 711 BsaBI GATnn'nnATC Cuts at: 5560 5560 Size: 5566 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3373 3755 4390 275 Size: 53 83 186 936 1840 382 635 1451 Fragments arranged by size: 1840 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2075 2370 2400 1178 Size: 30 165 30 642 30 295 30 4344 Fragments arranged by size: 4344 642 295 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 1186 Size: 1618 3948 BseYI C'CCAG_C Cuts at: 2134 4769 5265 2134 Size: 2635 496 2435 Fragments arranged by size: 2635 2435 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4993 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3016 3777 4805 1540 Size: 275 186 1015 761 1028 2301 Fragments arranged by size: 2301 1028 1015 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2690 3627 3712 4783 2690 Size: 937 85 1071 3473 Fragments arranged by size: 3473 1071 937 85 BsmI GAATG_Cn' Cuts at: 3077 3170 3077 Size: 93 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2464 2690 2885 3627 3712 4783 1391 Size: 399 674 226 195 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 399 226 195 85 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2921 844 Size: 1392 685 3489 Fragments arranged by size: 3489 1392 685 BsrBI CCG'CTC Cuts at: 1394 1823 3453 1394 Size: 429 1630 3507 Fragments arranged by size: 3507 1630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4061 4235 65 Size: 1183 2813 174 1396 Fragments arranged by size: 2813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4210 4397 1700 Size: 110 2400 187 2869 Fragments arranged by size: 2869 2400 187 110 BsrGI T'GTAC_A Cuts at: 96 2568 96 Size: 2472 3094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5566 BssSI C'ACGA_G Cuts at: 3626 4638 3626 Size: 1012 4554 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5566 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstYI r'GATC_y Cuts at: 1972 2130 2850 2896 3263 3648 3665 5117 5203 Size: 158 720 46 367 385 17 1452 86 Cuts at: 5203 5215 5561 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 720 385 367 346 158 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 513 Size: 552 939 75 4000 Fragments arranged by size: 4000 939 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4827 Fragments arranged by size: 4827 683 56 BtsI GCAGTG_nn' Cuts at: 2490 3082 3876 3896 2490 Size: 592 794 20 4160 Fragments arranged by size: 4160 794 592 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2242 2870 2242 Size: 628 4938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2407 4573 809 Size: 507 1091 2166 1802 Fragments arranged by size: 2166 1802 1091 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2169 3013 Size: 54 1425 50 275 18 180 159 844 Cuts at: 3013 3902 5531 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 844 275 180 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 1537 Size: 275 1201 4090 Fragments arranged by size: 4090 1201 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3496 1353 Size: 2143 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4153 4527 4673 387 Size: 1064 138 2564 374 146 1280 Fragments arranged by size: 2564 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2829 3629 4226 5013 5281 1239 Size: 393 415 782 800 597 787 268 1524 Fragments arranged by size: 1524 800 787 782 597 415 393 268 EcoO109I rG'GnC_Cy Cuts at: 2308 2308 Size: 5566 EcoRI G'AATT_C Cuts at: 2345 2875 3253 2345 Size: 530 378 4658 Fragments arranged by size: 4658 530 378 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 4713 1678 Size: 3035 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2800 2825 2832 2857 4207 Size: 32 135 32 1228 25 7 25 1350 Cuts at: 4207 4239 4281 4313 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 1228 135 42 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2910 3156 669 Size: 988 281 972 246 3079 Fragments arranged by size: 3079 988 972 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2692 4680 4864 1305 Size: 1387 1988 184 2007 Fragments arranged by size: 2007 1988 1387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 10 Size: 54 1425 343 339 3405 Fragments arranged by size: 3405 1425 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 3524 3883 4042 518 Size: 648 2358 359 159 2042 Fragments arranged by size: 2358 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 2177 2746 2982 3659 4807 5052 1606 Size: 532 39 569 236 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 569 532 245 236 39 NcoI C'CATG_G Cuts at: 513 1065 2004 2079 513 Size: 552 939 75 4000 Fragments arranged by size: 4000 939 552 75 NdeI CA'TA_TG Cuts at: 387 2701 387 Size: 2314 3252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5566 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NsiI A_TGCA'T Cuts at: 2725 2725 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2338 2924 4469 1132 Size: 97 1109 586 1545 2229 Fragments arranged by size: 2229 1545 1109 586 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5566 PciI A'CATG_T Cuts at: 4465 4465 Size: 5566 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 1108 Size: 911 4655 PfoI T'CCnGG_A Cuts at: 2215 2215 Size: 5566 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2045 2077 3635 3667 5148 5180 2045 Size: 32 1558 32 1481 32 2431 Fragments arranged by size: 2431 1558 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 5566 PshAI GACnn'nnGTC Cuts at: 1613 2494 1613 Size: 881 4685 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PstI C_TGCA'G Cuts at: 830 2918 830 Size: 2088 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5566 PvuII CAG'CTG Cuts at: 2138 2177 2746 2138 Size: 39 569 4958 Fragments arranged by size: 4958 569 39 SalI G'TCGA_C Cuts at: 1936 2908 1936 Size: 972 4594 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SfcI C'TryA_G Cuts at: 826 1045 2650 2656 2914 4049 4730 4921 826 Size: 219 1605 6 258 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 681 258 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2893 1872 Size: 1021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3670 4571 4833 820 Size: 197 926 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2338 2924 1132 Size: 1206 586 3774 Fragments arranged by size: 3774 1206 586 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5566 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 3005 513 Size: 552 939 75 926 3074 Fragments arranged by size: 3074 939 926 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3593 3610 3763 3948 4393 325 Size: 1255 2013 17 153 185 445 1498 Fragments arranged by size: 2013 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2568 3812 5524 Size: 274 80 33 51 1443 591 1244 1712 Cuts at: 5524 96 Size: 138 Fragments arranged by size: 1712 1443 1244 591 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2370 2402 5526 5558 2370 Size: 32 3124 32 2378 Fragments arranged by size: 3124 2378 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2743 2020 Size: 723 4843 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2891 1870 Size: 1021 4545 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI