(Circular) (Six-base) MAPSORT of: pFN21AE4108.seq Check: 1705 from: 1 to: 6139 pFN21AE4108 6139 bp With 182 enzymes: SgfI * March 28, 2012 13:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2707 2707 Size: 6139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2991 3949 278 Size: 53 83 186 936 1455 958 2468 Fragments arranged by size: 2468 1455 958 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 2061 3460 2061 Size: 1399 4740 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3116 4202 5586 5854 1632 Size: 1484 1086 1384 268 1917 Fragments arranged by size: 1917 1484 1384 1086 268 AfeI AGC'GCT Cuts at: 2216 2216 Size: 6139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2666 3012 3075 5038 6015 1203 Size: 1463 346 63 1963 977 1327 Fragments arranged by size: 1963 1463 1327 977 346 63 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 2669 2999 3146 1166 Size: 1503 330 147 4159 Fragments arranged by size: 4159 1503 330 147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 2521 3284 3394 3882 5454 2521 Size: 763 110 488 1572 3206 Fragments arranged by size: 3206 1572 763 488 110 ApaI G_GGCC'C Cuts at: 2509 2954 2509 Size: 445 5694 ApaLI G'TGCA_C Cuts at: 2406 3202 4196 5352 2406 Size: 796 994 1156 3193 Fragments arranged by size: 3193 1156 994 796 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2756 3041 3404 3464 1870 Size: 73 813 285 363 60 4545 Fragments arranged by size: 4545 813 363 285 73 60 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2475 3460 4915 618 Size: 325 217 627 274 414 985 1455 1842 Fragments arranged by size: 1842 1455 985 627 414 325 274 217 BanII G_rGCy'C Cuts at: 1391 2185 2257 2509 2694 2763 2902 2954 3458 Size: 794 72 252 185 69 139 52 504 Cuts at: 3458 1391 Size: 4072 Fragments arranged by size: 4072 794 504 252 185 139 72 69 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2983 928 Size: 971 1084 4084 Fragments arranged by size: 4084 1084 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2443 2477 4330 4364 1903 Size: 34 506 34 1853 34 3678 Fragments arranged by size: 3678 1853 506 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 5203 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2504 4750 136 Size: 107 122 71 631 605 832 2246 1525 Fragments arranged by size: 2246 1525 832 631 605 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 2606 3062 3556 2606 Size: 456 494 5189 Fragments arranged by size: 5189 494 456 Bme1580I G_kGCm'C Cuts at: 1790 2148 2233 2410 2509 2749 2954 3206 3403 Size: 358 85 177 99 240 205 252 197 Cuts at: 3403 4200 5356 1790 Size: 797 1156 2573 Fragments arranged by size: 2573 1156 797 358 252 240 205 197 177 99 85 BmgBI CAC'GTC Cuts at: 1318 2083 2353 1318 Size: 765 270 5104 Fragments arranged by size: 5104 765 270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3325 3325 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2636 4799 1239 Size: 851 546 2163 2579 Fragments arranged by size: 2579 2163 851 546 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3212 4264 5129 5427 3212 Size: 1052 865 298 3924 Fragments arranged by size: 3924 1052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2411 2667 3001 3343 3930 493 Size: 711 1207 256 334 342 587 2702 Fragments arranged by size: 2702 1207 711 587 342 334 256 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2651 2988 3946 4328 Size: 53 83 186 936 1118 337 958 382 Cuts at: 4328 4963 275 Size: 635 1451 Fragments arranged by size: 1451 1118 958 936 635 382 337 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2609 2639 1178 Size: 30 165 30 1206 30 4678 Fragments arranged by size: 4678 1206 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2945 3287 3392 1186 Size: 979 780 342 105 3933 Fragments arranged by size: 3933 979 780 342 105 BseYI C'CCAG_C Cuts at: 2956 5342 5838 2956 Size: 2386 496 3257 Fragments arranged by size: 3257 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3377 1163 Size: 573 1641 3925 Fragments arranged by size: 3925 1641 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3589 4350 5378 1540 Size: 275 186 1111 477 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 477 275 186 BsiHKAI G_wGCw'C Cuts at: 2410 2763 3006 3206 4200 4285 5356 2410 Size: 353 243 200 994 85 1071 3193 Fragments arranged by size: 3193 1071 994 353 243 200 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 BsmBI CGTCTCn'nnnn_ Cuts at: 2073 3296 2073 Size: 1223 4916 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2185 2233 2257 2410 2509 2694 Size: 399 358 37 48 24 153 99 185 Cuts at: 2694 2749 2763 2902 2954 3006 3206 3403 3458 Size: 55 14 139 52 52 200 197 55 Cuts at: 3458 4200 4285 5356 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 200 197 185 153 139 99 85 55 55 52 52 48 37 24 14 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2263 2610 2707 3090 3420 3494 844 Size: 1229 190 347 97 383 330 74 3489 Fragments arranged by size: 3489 1229 383 347 330 190 97 74 BsrBI CCG'CTC Cuts at: 1394 1823 3244 4026 1394 Size: 429 1421 782 3507 Fragments arranged by size: 3507 1421 782 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2591 4634 4808 65 Size: 1183 1343 2043 174 1396 Fragments arranged by size: 2043 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4783 4970 1700 Size: 110 2973 187 2869 Fragments arranged by size: 2973 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2523 3015 96 Size: 2427 492 3220 Fragments arranged by size: 3220 2427 492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 2147 3416 4199 5211 2147 Size: 1269 783 1012 3075 Fragments arranged by size: 3075 1269 1012 783 BstAPI GCAn_nnn'nTGC Cuts at: 794 2414 3394 794 Size: 1620 980 3539 Fragments arranged by size: 3539 1620 980 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2434 3430 2434 Size: 996 5143 BstYI r'GATC_y Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134 Size: 1497 367 385 17 1452 86 12 346 Cuts at: 6134 1972 Size: 1977 Fragments arranged by size: 1977 1497 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2944 2944 Size: 6139 BtgI C'CryG_G Cuts at: 513 1065 2004 2493 513 Size: 552 939 489 4159 Fragments arranged by size: 4159 939 552 489 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2375 487 Size: 683 56 1149 4251 Fragments arranged by size: 4251 1149 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3655 4449 4469 2890 Size: 765 794 20 4560 Fragments arranged by size: 4560 794 765 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DraIII CAC_nnn'GTG Cuts at: 2237 2414 2237 Size: 177 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2577 2736 Size: 54 1425 50 275 18 342 405 159 Cuts at: 2736 2878 3109 3165 3216 3345 3586 4475 6104 Size: 142 231 56 51 129 241 889 1629 Cuts at: 6104 8 Size: 43 Fragments arranged by size: 1629 1425 889 405 342 275 241 231 159 142 129 56 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 3586 1537 Size: 275 1297 477 4090 Fragments arranged by size: 4090 1297 477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3092 3179 4069 1353 Size: 1739 87 890 3423 Fragments arranged by size: 3423 1739 890 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 3167 4726 5100 5246 387 Size: 1064 138 648 930 1559 374 146 1280 Fragments arranged by size: 1559 1280 1064 930 648 374 146 138 EcoICRI GAG'CTC Cuts at: 2761 2761 Size: 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2636 3116 4202 4799 5586 5854 Size: 393 458 546 480 1086 597 787 268 Cuts at: 5854 1239 Size: 1524 Fragments arranged by size: 1524 1086 787 597 546 480 458 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2717 2290 Size: 427 5712 EcoO109I rG'GnC_Cy Cuts at: 2505 2506 2599 2950 2951 3409 2505 Size: 1 93 351 1 458 5235 Fragments arranged by size: 5235 458 351 93 1 1 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2218 2575 2732 3372 5286 1678 Size: 540 357 157 640 1914 2531 Fragments arranged by size: 2531 1914 640 540 357 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 4780 4812 4854 Size: 32 135 32 1049 32 2127 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 2127 1049 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 2065 3464 2065 Size: 1399 4740 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2579 3167 3218 3347 Size: 54 1425 343 342 405 588 51 129 Cuts at: 3347 10 Size: 2802 Fragments arranged by size: 2802 1425 588 405 343 342 129 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2559 2669 2999 3146 4097 4456 4615 Size: 648 1393 110 330 147 951 359 159 Cuts at: 4615 518 Size: 2042 Fragments arranged by size: 2042 1393 951 648 359 330 159 147 110 MspA1I CmG'CkG Cuts at: 1606 2031 2187 2323 2593 2814 3281 3319 3555 Size: 425 156 136 270 221 467 38 236 Cuts at: 3555 4232 5380 5625 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 467 425 270 245 236 221 156 136 38 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3109 3586 3109 Size: 477 5662 NspI r_CATG'y Cuts at: 1132 1229 2434 2558 3016 3079 3497 5042 1132 Size: 97 1205 124 458 63 418 1545 2229 Fragments arranged by size: 2229 1545 1205 458 418 124 97 63 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 3012 3075 5038 3012 Size: 63 1963 4113 Fragments arranged by size: 4113 1963 63 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PfoI T'CCnGG_A Cuts at: 2715 2715 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PmlI CAC'GTG Cuts at: 2667 3001 3343 2667 Size: 334 342 5463 Fragments arranged by size: 5463 342 334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 3409 2599 Size: 810 5329 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 2505 2950 2505 Size: 445 5694 PspXI vC'TCGA_Gb Cuts at: 3404 3404 Size: 6139 PstI C_TGCA'G Cuts at: 830 2036 2291 2517 2704 2849 3491 830 Size: 1206 255 226 187 145 642 3478 Fragments arranged by size: 3478 1206 642 255 226 187 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2031 2593 2814 2031 Size: 562 221 5356 Fragments arranged by size: 5356 562 221 SacI G_AGCT'C Cuts at: 2763 2763 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SapI GCTCTTCn'nnn_ Cuts at: 3179 3179 Size: 6139 SbfI CC_TGCA'GG Cuts at: 2291 3491 2291 Size: 1200 4939 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 2032 2287 2513 2700 2845 3364 3487 Size: 219 987 255 226 187 145 519 123 Cuts at: 3487 4622 5303 5494 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 987 681 519 255 226 219 191 187 145 123 SfiI GGCCn_nnn'nGGCC Cuts at: 2504 2504 Size: 6139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3191 3404 4243 5144 5406 820 Size: 197 926 1248 213 839 901 262 1553 Fragments arranged by size: 1553 1248 926 901 839 262 213 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2867 3093 1517 Size: 1350 226 4563 Fragments arranged by size: 4563 1350 226 StyI C'CwwG_G Cuts at: 513 1065 2004 3219 3578 513 Size: 552 939 1215 359 3074 Fragments arranged by size: 3074 1215 939 552 359 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2393 2493 3140 3416 4166 4183 4336 Size: 1255 813 100 647 276 750 17 153 Cuts at: 4336 4521 4966 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 813 750 647 445 276 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2365 2523 Size: 274 80 33 51 1443 147 241 158 Cuts at: 2523 3015 3210 4385 6097 96 Size: 492 195 1175 1712 138 Fragments arranged by size: 1712 1443 1175 492 274 241 195 158 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2609 2641 6099 6131 2609 Size: 32 3458 32 2617 Fragments arranged by size: 3458 2617 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2354 1316 Size: 1038 5101 XbaI T'CTAG_A Cuts at: 2329 3475 2329 Size: 1146 4993 XhoI C'TCGA_G Cuts at: 1943 3404 1943 Size: 1461 4678 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 2378 4268 2378 Size: 1890 4249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2989 3947 276 Size: 53 83 186 936 1455 958 2468 Fragments arranged by size: 2468 1455 958 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI