(Circular) (Six-base) MAPSORT of: pFN21AE0694.seq Check: 851 from: 1 to: 5914 pFN21AE0694 5914 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 3257 1937 Size: 831 489 4594 Fragments arranged by size: 4594 831 489 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 2886 3977 5361 5629 1632 Size: 759 495 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 759 495 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2439 1184 Size: 1255 4659 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaI G_GGCC'C Cuts at: 2087 2087 Size: 5914 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 2052 2263 2874 3000 3223 3449 3601 5710 Size: 559 211 611 126 223 226 152 2109 Cuts at: 5710 1493 Size: 1697 Fragments arranged by size: 2109 1697 611 559 226 223 211 152 126 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 3239 1870 Size: 73 1296 4545 Fragments arranged by size: 4545 1296 73 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 3235 4690 618 Size: 325 217 627 465 983 1455 1842 Fragments arranged by size: 1842 1455 983 627 465 325 217 BanII G_rGCy'C Cuts at: 1391 2087 3233 1391 Size: 696 1146 4072 Fragments arranged by size: 4072 1146 696 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2634 928 Size: 971 735 4208 Fragments arranged by size: 4208 971 735 BbvCI CC'TCA_GC Cuts at: 2673 2692 2673 Size: 19 5895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BfrBI ATG'CAT Cuts at: 2420 2420 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2961 4525 136 Size: 107 122 71 631 605 1289 1564 1525 Fragments arranged by size: 1564 1525 1289 631 605 122 107 71 BglII A'GATC_T Cuts at: 3208 5909 3208 Size: 2701 3213 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 2087 2160 3975 5131 1790 Size: 297 73 1815 1156 2573 Fragments arranged by size: 2573 1815 1156 297 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2343 2812 3645 Size: 416 53 83 399 24 926 469 833 Cuts at: 3645 4603 442 Size: 958 1753 Fragments arranged by size: 1753 958 926 833 469 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2955 1056 Size: 1899 4015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4574 1239 Size: 3335 2579 Bpu10I CC'TnA_GC Cuts at: 2673 2692 2673 Size: 19 5895 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4039 4904 5202 4039 Size: 865 298 4751 Fragments arranged by size: 4751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 4577 882 Size: 617 537 2541 2219 Fragments arranged by size: 2541 2219 617 537 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3721 4103 4738 Size: 53 83 186 936 476 1712 382 635 Cuts at: 4738 275 Size: 1451 Fragments arranged by size: 1712 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3084 3114 1178 Size: 30 165 30 1681 30 3978 Fragments arranged by size: 3978 1681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5914 BseYI C'CCAG_C Cuts at: 2013 2311 5117 5613 2013 Size: 298 2806 496 2314 Fragments arranged by size: 2806 2314 496 298 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3975 4060 5131 3975 Size: 85 1071 4758 Fragments arranged by size: 4758 1071 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2160 3233 3975 4060 5131 1391 Size: 399 297 73 1073 742 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 399 297 85 73 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 2992 3269 844 Size: 1386 762 277 3489 Fragments arranged by size: 3489 1386 762 277 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4409 4583 65 Size: 1183 3161 174 1396 Fragments arranged by size: 3161 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstYI r'GATC_y Cuts at: 1972 3208 3244 3611 3996 4013 5465 5551 5563 Size: 1236 36 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3096 513 Size: 552 939 1092 3331 Fragments arranged by size: 3331 1092 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 2209 3430 4224 4244 2209 Size: 1221 794 20 3879 Fragments arranged by size: 3879 1221 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2917 2952 529 Size: 35 2353 35 3491 Fragments arranged by size: 3491 2353 35 35 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2705 2962 3361 Size: 54 1425 50 275 18 875 257 399 Cuts at: 3361 4250 5879 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 875 399 275 257 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2605 3844 1353 Size: 1252 1239 3423 Fragments arranged by size: 3423 1252 1239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2391 2886 3977 4574 5361 5629 1239 Size: 393 759 495 1091 597 787 268 1524 Fragments arranged by size: 1524 1091 787 759 597 495 393 268 EcoO109I rG'GnC_Cy Cuts at: 2084 2111 2084 Size: 27 5887 EcoRI G'AATT_C Cuts at: 2052 3223 3601 2052 Size: 1171 378 4365 Fragments arranged by size: 4365 1171 378 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373 Size: 32 135 32 2983 32 42 32 2626 Fragments arranged by size: 2983 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5028 5212 1305 Size: 3723 184 2007 Fragments arranged by size: 3723 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2707 2964 10 Size: 54 1425 343 875 257 2960 Fragments arranged by size: 2960 1425 875 343 257 54 MslI CAynn'nnrTG Cuts at: 518 1166 2778 3872 4231 4390 518 Size: 648 1612 1094 359 159 2042 Fragments arranged by size: 2042 1612 1094 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 2593 3330 4007 5155 5400 1606 Size: 856 131 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 737 677 245 131 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 2951 1052 Size: 1899 4015 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NsiI A_TGCA'T Cuts at: 2422 2422 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2420 2664 3272 4817 1132 Size: 97 1191 244 608 1545 2229 Fragments arranged by size: 2229 1545 1191 608 244 97 PasI CC'CwG_GG Cuts at: 1348 2037 1348 Size: 689 5225 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 2037 1108 Size: 929 4985 PfoI T'CCnGG_A Cuts at: 2056 2056 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2111 Size: 5914 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PspOMI G'GGCC_C Cuts at: 2083 2083 Size: 5914 PstI C_TGCA'G Cuts at: 830 3266 830 Size: 2436 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2462 2462 Size: 5914 RsrII CG'GwC_CG Cuts at: 2066 2066 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2281 2588 3262 4397 5078 5269 826 Size: 219 1236 307 674 1135 681 191 1471 Fragments arranged by size: 1471 1236 1135 681 674 307 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 4919 5181 820 Size: 197 926 2075 901 262 1553 Fragments arranged by size: 2075 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 2420 2664 3272 1132 Size: 1288 244 608 3774 Fragments arranged by size: 3774 1288 608 244 SspI AAT'ATT Cuts at: 5 52 2712 3838 5 Size: 47 2660 1126 2081 Fragments arranged by size: 2660 2081 1126 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2004 2174 2665 2734 3353 513 Size: 552 939 170 491 69 619 3074 Fragments arranged by size: 3074 939 619 552 491 170 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2362 2694 3941 3958 4111 4296 4741 Size: 1255 782 332 1247 17 153 185 445 Cuts at: 4741 325 Size: 1498 Fragments arranged by size: 1498 1255 1247 782 445 332 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4160 5872 96 Size: 274 80 33 51 1443 2183 1712 138 Fragments arranged by size: 2183 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI