Restriction map for
pFN21AB0424
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BcgI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MamI MfeI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AclI 132 AACGTT AA^CGTT
AcyI 843 GGCGCC G[GA]^CG[CT]C
AflII 2493 CTTAAG C^TTAAG
AflIII 1764 ACGCGT A^C[GA][CT]GT
AocI 871 CCTCAGG CC^T[ACGT]AGG
Aor51HI 152 AGCGCT AGC^GCT
BalI 2262 TGGCCA TGG^CCA
BamHI 876 GGATCC G^GATCC
BanI 843 GGCGCC G^G[CT][GA]CC
BanII 796 GAGCTC G[GA]GC[CT]^C
BbeI 843 GGCGCC GGCGC^C
BbvCI 234 GCTGAGG CCTCAGC(-5/-2)
BciVI 673 GTATCC GTATCC(6/5)
BfaI 1564 CTAG C^TAG
BfrI 2493 CTTAAG C^TTAAG
BsaHI 843 GGCGCC G[GA]^CG[CT]C
BsiEI 1317 CGGTCG CG[GA][CT]^CG
BsmBI 535 CGTCTC CGTCTC(1/5)
BspHI 2325 TCATGA T^CATGA
BspMI 927 GCAGGT ACCTGC(4/8)
BsrDI 1295 GCAATG GCAATG(2/0)
BstXI 1938 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 871 CCTCAGG CC^T[ACGT]AGG
DraI 2449 TTTAAA TTT^AAA
DraIII 835 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 152 AGCGCT AGC^GCT
Eco81I 871 CCTCAGG CC^T[ACGT]AGG
EcoNI 323 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1544 GAATTC G^AATTC
EcoRV 2158 GATATC GAT^ATC
EcoT14I 767 CCATGG C^C[AT][AT]GG
Hin1I 843 GGCGCC G[GA]^CG[CT]C
HincII 931 GTCAAC GT[CT]^[GA]AC
HindII 931 GTCAAC GT[CT]^[GA]AC
HindIII 1668 AAGCTT A^AGCTT
KasI 843 GGCGCC G^GCGCC
MaeI 1564 CTAG C^TAG
MluI 1764 ACGCGT A^CGCGT
MluNI 2262 TGGCCA TGG^CCA
MscI 2262 TGGCCA TGG^CCA
NarI 843 GGCGCC GG^CGCC
NcoI 767 CCATGG C^CATGG
NdeI 1607 CATATG CA^TATG
PflMI 633 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 132 AACGTT AA^CGTT
PstI 477 CTGCAG CTGCA^G
RcaI 2325 TCATGA T^CATGA
SacI 796 GAGCTC GAGCT^C
SapI 1140 GAAGAGC GCTCTTC(1/4)
SexAI 1247 ACCTGGT A^CC[AT]GGT
SfoI 843 GGCGCC GGC^GCC
SmaI 191 CCCGGG CCC^GGG
SspI 1433 AATATT AAT^ATT
StyI 767 CCATGG C^C[AT][AT]GG
Van91I 633 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 782 CCAGGGACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 191 CCCGGG C^CCGGG
XmaI 191 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 9 enzymes) #
###################################
DsaI 497 CCGTGG C^C[GA][CT]GG
767 CCATGG
EaeI 365 TGGCCG [CT]^GGCC[GA]
2262 TGGCCA
FauI 126 CCCGC CCCGC(4/6)
1155 GCGGG
HgaI 1181 GCGTC GACGC(5/10)
1790 GCGTC
MaeII 133 ACGT A^CGT
2018 ACGT
PvuII 44 CAGCTG CAG^CTG
2430 CAGCTG
SfcI 477 CTGCAG C^T[GA][CT]AG
1593 CTACAG
TaqI 1337 TCGA T^CGA
1956 TCGA
TthHB8I 1337 TCGA T^CGA
1956 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI