(Circular) (Six-base) MAPSORT of: pFN21AB0424.seq Check: 2410 from: 1 to: 7222 pFN21AB0424 7222 bp With 182 enzymes: SgfI * March 28, 2012 13:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5032 278 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4565 1937 Size: 2628 4594 Acc65I G'GTAC_C Cuts at: 4543 4543 Size: 7222 AclI AA'CG_TT Cuts at: 1563 2138 5349 5722 1563 Size: 575 3211 373 3063 Fragments arranged by size: 3211 3063 575 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2172 2334 2612 3346 3829 4376 5285 Size: 514 26 162 278 734 483 547 909 Cuts at: 5285 6669 6937 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 909 734 547 514 483 278 268 162 26 AfeI AGC'GCT Cuts at: 2159 2159 Size: 7222 AflII C'TTAA_G Cuts at: 820 1017 4498 820 Size: 197 3481 3544 Fragments arranged by size: 3544 3481 197 AflIII A'CryG_T Cuts at: 1203 3769 6121 7098 1203 Size: 2566 2352 977 1327 Fragments arranged by size: 2566 2352 1327 977 AgeI A'CCGG_T Cuts at: 6053 6053 Size: 7222 AhdI GACnn_n'nnGTC Cuts at: 5951 5951 Size: 7222 AleI CACnn'nnGTG Cuts at: 1166 3267 3804 1166 Size: 2101 537 4584 Fragments arranged by size: 4584 2101 537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 2439 1184 Size: 948 307 5967 Fragments arranged by size: 5967 948 307 AlwNI CAG_nnn'CTG Cuts at: 2272 2299 2608 4965 6537 2272 Size: 27 309 2357 1572 2957 Fragments arranged by size: 2957 2357 1572 309 27 ApaLI G'TGCA_C Cuts at: 3164 3687 3773 3881 5279 6435 3164 Size: 523 86 108 1398 1156 3951 Fragments arranged by size: 3951 1398 1156 523 108 86 ApoI r'AATT_y Cuts at: 1493 3062 3429 3549 4429 4531 4757 4909 7018 Size: 1569 367 120 880 102 226 152 2109 Cuts at: 7018 1493 Size: 1697 Fragments arranged by size: 2109 1697 1569 880 367 226 152 120 102 AseI AT'TA_AT Cuts at: 160 1032 5776 160 Size: 872 4744 1606 Fragments arranged by size: 4744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2196 2246 3972 4547 1870 Size: 73 79 174 50 1726 575 4545 Fragments arranged by size: 4545 1726 575 174 79 73 50 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2912 2945 2912 Size: 33 7189 BamHI G'GATC_C Cuts at: 2881 4552 4919 2881 Size: 1671 367 5184 Fragments arranged by size: 5184 1671 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2848 4543 5998 618 Size: 325 217 627 1061 1695 1455 1842 Fragments arranged by size: 1842 1695 1455 1061 627 325 217 BanII G_rGCy'C Cuts at: 1391 2805 4541 1391 Size: 1414 1736 4072 Fragments arranged by size: 4072 1736 1414 BbeI G_GCGC'C Cuts at: 2852 2852 Size: 7222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 2109 4128 928 Size: 971 180 30 2019 4022 Fragments arranged by size: 4022 2019 971 180 30 BbvCI CC'TCA_GC Cuts at: 2240 2240 Size: 7222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5413 5447 1903 Size: 34 3476 34 3678 Fragments arranged by size: 3678 3476 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2689 5114 6324 2689 Size: 2425 1210 3587 Fragments arranged by size: 3587 2425 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7222 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5833 136 Size: 107 122 71 631 605 4161 1525 Fragments arranged by size: 4161 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7217 7217 Size: 7222 BlpI GC'TnA_GC Cuts at: 4639 4639 Size: 7222 Bme1580I G_kGCm'C Cuts at: 1790 3042 3168 3691 3777 3885 5283 6439 1790 Size: 1252 126 523 86 108 1398 1156 2573 Fragments arranged by size: 2573 1398 1252 1156 523 126 108 86 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2640 2851 3729 Size: 416 53 83 399 24 1223 211 878 Cuts at: 3729 4953 5911 442 Size: 1224 958 1753 Fragments arranged by size: 1753 1224 1223 958 878 416 399 211 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2892 2892 Size: 7222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2309 2484 2567 2711 3132 3657 4291 Size: 1055 15 175 83 144 421 525 634 Cuts at: 4291 4459 5882 1239 Size: 168 1423 2579 Fragments arranged by size: 2579 1423 1055 634 525 421 175 168 144 83 15 Bpu10I CC'TnA_GC Cuts at: 2240 2517 3012 2240 Size: 277 495 6450 Fragments arranged by size: 6450 495 277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 3474 5347 6212 6510 2420 Size: 1054 1873 865 298 3132 Fragments arranged by size: 3132 1873 1054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3637 3889 4518 5885 882 Size: 617 2138 252 629 1367 2219 Fragments arranged by size: 2219 2138 1367 629 617 252 BsaAI yAC'GTr Cuts at: 493 1204 5013 493 Size: 711 3809 2702 Fragments arranged by size: 3809 2702 711 BsaBI GATnn'nnATC Cuts at: 7216 7216 Size: 7222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2849 5029 5411 6046 Size: 53 83 186 936 1316 2180 382 635 Cuts at: 6046 275 Size: 1451 Fragments arranged by size: 2180 1451 1316 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5655 6053 6327 6474 6885 5655 Size: 398 274 147 411 5992 Fragments arranged by size: 5992 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6997 Fragments arranged by size: 6997 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7222 BseYI C'CCAG_C Cuts at: 2047 2511 3174 6425 6921 2047 Size: 464 663 3251 496 2348 Fragments arranged by size: 3251 2348 663 496 464 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2092 2237 2492 1163 Size: 573 356 145 255 5893 Fragments arranged by size: 5893 573 356 255 145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4672 5433 6461 1540 Size: 275 186 1324 1347 761 1028 2301 Fragments arranged by size: 2301 1347 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2805 3168 3691 3777 3885 4050 5283 5368 Size: 579 363 523 86 108 165 1233 85 Cuts at: 5368 6439 2226 Size: 1071 3009 Fragments arranged by size: 3009 1233 1071 579 523 363 165 108 86 85 BsmI GAATG_Cn' Cuts at: 4733 4826 4733 Size: 93 7129 BsmBI CGTCTCn'nnnn_ Cuts at: 2546 2546 Size: 7222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2805 3042 3168 3691 3777 3885 Size: 399 436 579 237 126 523 86 108 Cuts at: 3885 4050 4541 5283 5368 6439 1391 Size: 165 491 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 523 491 436 399 237 165 126 108 86 85 BspEI T'CCGG_A Cuts at: 6885 6885 Size: 7222 BspHI T'CATG_A Cuts at: 4330 5111 6841 4330 Size: 781 1730 4711 Fragments arranged by size: 4711 1730 781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2923 4577 844 Size: 2079 1654 3489 Fragments arranged by size: 3489 2079 1654 BsrBI CCG'CTC Cuts at: 1394 1823 5109 1394 Size: 429 3286 3507 Fragments arranged by size: 3507 3286 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3307 5717 5891 65 Size: 1183 2059 2410 174 1396 Fragments arranged by size: 2410 2059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5866 6053 1700 Size: 110 4056 187 2869 Fragments arranged by size: 4056 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7222 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7222 BssSI C'ACGA_G Cuts at: 5282 6294 5282 Size: 1012 6210 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7222 BstBI TT'CG_AA Cuts at: 6026 6026 Size: 7222 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7222 BstXI CCAn_nnnn'nTGG Cuts at: 3950 3950 Size: 7222 BstYI r'GATC_y Cuts at: 1972 2268 2881 2983 4483 4552 4919 5304 5321 Size: 296 613 102 1500 69 367 385 17 Cuts at: 5321 6773 6859 6871 7217 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1500 1452 613 385 367 346 296 102 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2877 2877 Size: 7222 BtgI C'CryG_G Cuts at: 513 1065 2502 2772 513 Size: 552 1437 270 4963 Fragments arranged by size: 4963 1437 552 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6483 Fragments arranged by size: 6483 683 56 BtsI GCAGTG_nn' Cuts at: 2475 3391 4738 5532 5552 2475 Size: 916 1347 794 20 4145 Fragments arranged by size: 4145 1347 916 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7222 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3289 3324 529 Size: 35 2725 35 4427 Fragments arranged by size: 4427 2725 35 35 DraI TTT'AAA Cuts at: 4456 4526 4456 Size: 70 7152 DraIII CAC_nnn'GTG Cuts at: 2845 2845 Size: 7222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6229 809 Size: 507 4913 1802 Fragments arranged by size: 4913 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 4267 4669 Size: 54 1425 50 275 18 540 1897 402 Cuts at: 4669 5558 7187 8 Size: 889 1629 43 Fragments arranged by size: 1897 1629 1425 889 540 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4669 1537 Size: 275 2857 4090 Fragments arranged by size: 4090 2857 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 2355 2652 3140 5152 1353 Size: 957 45 297 488 2012 3423 Fragments arranged by size: 3423 2012 957 488 297 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5809 6183 6329 387 Size: 1064 138 4220 374 146 1280 Fragments arranged by size: 4220 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2803 2803 Size: 7222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2172 2294 2309 2334 2484 2567 Size: 393 514 26 122 15 25 150 83 Cuts at: 2567 2612 2711 3132 3346 3657 3829 4291 4376 Size: 45 99 421 214 311 172 462 85 Cuts at: 4376 4459 5285 5882 6669 6937 1239 Size: 83 826 597 787 268 1524 Fragments arranged by size: 1524 826 787 597 514 462 421 393 311 268 214 172 150 122 99 85 83 83 45 26 25 15 EcoNI CCTnn'n_nnAGG Cuts at: 2332 2332 Size: 7222 EcoO109I rG'GnC_Cy Cuts at: 2192 2634 3288 3855 2192 Size: 442 654 567 5559 Fragments arranged by size: 5559 654 567 442 EcoRI G'AATT_C Cuts at: 3549 4531 4909 3549 Size: 982 378 5862 Fragments arranged by size: 5862 982 378 EcoRV GAT'ATC Cuts at: 4165 4165 Size: 7222 FspI TGC'GCA Cuts at: 5728 5728 Size: 7222 HaeII r_GCGC'y Cuts at: 1678 2161 2528 2852 6369 1678 Size: 483 367 324 3517 2531 Fragments arranged by size: 3517 2531 483 367 324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 2819 2851 3139 Size: 32 135 32 754 32 461 32 288 Cuts at: 3139 3171 3676 3708 4468 4500 5863 5895 5937 Size: 32 505 32 760 32 1363 32 42 Cuts at: 5937 5969 1373 Size: 32 2626 Fragments arranged by size: 2626 1363 760 754 505 461 288 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2938 4566 4812 669 Size: 988 281 1000 1628 246 3079 Fragments arranged by size: 3079 1628 1000 988 281 246 HindIII A'AGCT_T Cuts at: 748 3673 748 Size: 2925 4297 HpaI GTT'AAC Cuts at: 4812 4812 Size: 7222 KasI G'GCGC_C Cuts at: 2848 2848 Size: 7222 KpnI G_GTAC'C Cuts at: 4547 4547 Size: 7222 MfeI C'AATT_G Cuts at: 4821 4821 Size: 7222 MluI A'CGCG_T Cuts at: 3769 7098 3769 Size: 3329 3893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4082 6336 6520 1305 Size: 2777 2254 184 2007 Fragments arranged by size: 2777 2254 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4269 10 Size: 54 1425 343 2437 2963 Fragments arranged by size: 2963 2437 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2114 3267 3652 3736 3804 4335 Size: 648 843 105 1153 385 84 68 531 Cuts at: 4335 4392 5180 5539 5698 518 Size: 57 788 359 159 2042 Fragments arranged by size: 2042 1153 843 788 648 531 385 359 159 105 84 68 57 MspA1I CmG'CkG Cuts at: 1606 2051 3160 3279 4437 4638 5315 6463 6708 Size: 445 1109 119 1158 201 677 1148 245 Cuts at: 6708 1606 Size: 2120 Fragments arranged by size: 2120 1158 1148 1109 677 445 245 201 119 NarI GG'CG_CC Cuts at: 2849 2849 Size: 7222 NcoI C'CATG_G Cuts at: 513 1065 2772 513 Size: 552 1707 4963 Fragments arranged by size: 4963 1707 552 NdeI CA'TA_TG Cuts at: 387 3613 387 Size: 3226 3996 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7222 NotI GC'GGCC_GC Cuts at: 4669 4669 Size: 7222 NspI r_CATG'y Cuts at: 1132 1229 4580 6125 1132 Size: 97 3351 1545 2229 Fragments arranged by size: 3351 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2328 1348 Size: 980 6242 PciI A'CATG_T Cuts at: 6121 6121 Size: 7222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 5686 PfoI T'CCnGG_A Cuts at: 2271 2544 2688 2271 Size: 273 144 6805 Fragments arranged by size: 6805 273 144 PmeI GTTT'AAAC Cuts at: 4526 4526 Size: 7222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5291 5323 6804 6836 5291 Size: 32 1481 32 5677 Fragments arranged by size: 5677 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2192 2634 3855 2192 Size: 442 1221 5559 Fragments arranged by size: 5559 1221 442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7222 PsiI TTA'TAA Cuts at: 4792 4792 Size: 7222 PstI C_TGCA'G Cuts at: 830 2486 4574 830 Size: 1656 2088 3478 Fragments arranged by size: 3478 2088 1656 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7222 PvuII CAG'CTG Cuts at: 2051 4437 2051 Size: 2386 4836 SacI G_AGCT'C Cuts at: 2805 2805 Size: 7222 SalI G'TCGA_C Cuts at: 1936 4564 1936 Size: 2628 4594 SanDI GG'GwC_CC Cuts at: 2634 2634 Size: 7222 SapI GCTCTTCn'nnn_ Cuts at: 3140 3140 Size: 7222 SbfI CC_TGCA'GG Cuts at: 4574 4574 Size: 7222 ScaI AGT'ACT Cuts at: 5470 7182 5470 Size: 1712 5510 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 7222 SfcI C'TryA_G Cuts at: 826 1045 2482 3598 4570 5705 6386 6577 826 Size: 219 1437 1116 972 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 1116 972 681 219 191 SfoI GGC'GCC Cuts at: 2850 2850 Size: 7222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7222 SmaI CCC'GGG Cuts at: 1872 2198 4549 1872 Size: 326 2351 4545 Fragments arranged by size: 4545 2351 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3489 4498 5326 6227 6489 Size: 197 926 456 1090 1009 828 901 262 Cuts at: 6489 820 Size: 1553 Fragments arranged by size: 1553 1090 1009 926 901 828 456 262 197 SnaBI TAC'GTA Cuts at: 493 5013 493 Size: 4520 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7222 SphI G_CATG'C Cuts at: 1132 4580 1132 Size: 3448 3774 SspI AAT'ATT Cuts at: 5 52 3440 5146 5 Size: 47 3388 1706 2081 Fragments arranged by size: 3388 2081 1706 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7222 StyI C'CwwG_G Cuts at: 513 1065 2772 4661 513 Size: 552 1707 1889 3074 Fragments arranged by size: 3074 1889 1707 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2320 2525 2833 3188 3311 5249 Size: 1255 450 290 205 308 355 123 1938 Cuts at: 5249 5266 5419 5604 6049 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1938 1498 1255 450 445 355 308 290 205 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 5468 7180 Size: 274 80 33 51 1443 147 3344 1712 Cuts at: 7180 96 Size: 138 Fragments arranged by size: 3344 1712 1443 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3611 3643 7182 7214 3611 Size: 32 3539 32 3619 Fragments arranged by size: 3619 3539 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7222 XbaI T'CTAG_A Cuts at: 4558 4558 Size: 7222 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 2794 Size: 7222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7222 XmaI C'CCGG_G Cuts at: 1870 2196 4547 1870 Size: 326 2351 4545 Fragments arranged by size: 4545 2351 326 XmnI GAAnn'nnTTC Cuts at: 3317 3484 3674 3731 3842 5351 3317 Size: 167 190 57 111 1509 5188 Fragments arranged by size: 5188 1509 190 167 111 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5030 276 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI SwaI