(Linear) (Six-base) MAPSORT of: KIBB0424.seq Check: 3723 from: 1 to: 2517 KIBB0424.orf With 182 enzymes: SgfI * March 22, 2012 15:30 .. AclI AA'CG_TT Cuts at: 0 133 2517 Size: 133 2384 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 167 329 607 1341 1824 2371 2517 Size: 141 26 162 278 734 483 547 146 Fragments arranged by size: 734 547 483 278 162 146 141 26 AfeI AGC'GCT Cuts at: 0 154 2517 Size: 154 2363 AflII C'TTAA_G Cuts at: 0 2493 2517 Size: 2493 24 AflIII A'CryG_T Cuts at: 0 1764 2517 Size: 1764 753 AleI CACnn'nnGTG Cuts at: 0 1262 1799 2517 Size: 1262 537 718 Fragments arranged by size: 1262 718 537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 127 434 2517 Size: 127 307 2083 Fragments arranged by size: 2083 307 127 AlwNI CAG_nnn'CTG Cuts at: 0 267 294 603 2517 Size: 267 27 309 1914 Fragments arranged by size: 1914 309 267 27 ApaLI G'TGCA_C Cuts at: 0 1159 1682 1768 1876 2517 Size: 1159 523 86 108 641 Fragments arranged by size: 1159 641 523 108 86 ApoI r'AATT_y Cuts at: 0 1057 1424 1544 2424 2517 Size: 1057 367 120 880 93 Fragments arranged by size: 1057 880 367 120 93 AvaI C'yCGr_G Cuts at: 0 17 191 241 1967 2517 Size: 17 174 50 1726 550 Fragments arranged by size: 1726 550 174 50 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 907 940 2517 Size: 907 33 1577 Fragments arranged by size: 1577 907 33 BamHI G'GATC_C Cuts at: 0 876 2517 Size: 876 1641 BanI G'GyrC_C Cuts at: 0 843 2517 Size: 843 1674 BanII G_rGCy'C Cuts at: 0 800 2517 Size: 800 1717 BbeI G_GCGC'C Cuts at: 0 847 2517 Size: 847 1670 BbsI GAAGACnn'nnnn_ Cuts at: 0 74 104 2123 2517 Size: 74 30 2019 394 Fragments arranged by size: 2019 394 74 30 BbvCI CC'TCA_GC Cuts at: 0 235 2517 Size: 235 2282 BciVI GTATCCnnnnn_n' Cuts at: 0 684 2517 Size: 684 1833 Bme1580I G_kGCm'C Cuts at: 0 1037 1163 1686 1772 1880 2517 Size: 1037 126 523 86 108 637 Fragments arranged by size: 1037 637 523 126 108 86 BmrI ACTGGGnnnn_n' Cuts at: 0 635 846 1724 2517 Size: 635 211 878 793 Fragments arranged by size: 878 793 635 211 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 887 2517 Size: 887 1630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 304 479 562 706 1127 1652 2286 Size: 289 15 175 83 144 421 525 634 Cuts at: 2286 2454 2517 Size: 168 63 Fragments arranged by size: 634 525 421 289 175 168 144 83 63 15 Bpu10I CC'TnA_GC Cuts at: 0 235 512 1007 2517 Size: 235 277 495 1510 Fragments arranged by size: 1510 495 277 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1469 2517 Size: 415 1054 1048 Fragments arranged by size: 1054 1048 415 BsaI GGTCTCn'nnnn_ Cuts at: 0 1632 1884 2513 2517 Size: 1632 252 629 4 Fragments arranged by size: 1632 629 252 4 BsaHI Gr'CG_yC Cuts at: 0 844 2517 Size: 844 1673 BseYI C'CCAG_C Cuts at: 0 42 506 1169 2517 Size: 42 464 663 1348 Fragments arranged by size: 1348 663 464 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 87 232 487 2517 Size: 87 145 255 2030 Fragments arranged by size: 2030 255 145 87 BsiEI CG_ry'CG Cuts at: 0 1320 2517 Size: 1320 1197 BsiHKAI G_wGCw'C Cuts at: 0 221 800 1163 1686 1772 1880 2045 2517 Size: 221 579 363 523 86 108 165 472 Fragments arranged by size: 579 523 472 363 221 165 108 86 BsmBI CGTCTCn'nnnn_ Cuts at: 0 541 2517 Size: 541 1976 Bsp1286I G_dGCh'C Cuts at: 0 221 800 1037 1163 1686 1772 1880 2045 Size: 221 579 237 126 523 86 108 165 Cuts at: 2045 2517 Size: 472 Fragments arranged by size: 579 523 472 237 221 165 126 108 86 BspHI T'CATG_A Cuts at: 0 2325 2517 Size: 2325 192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 918 2517 Size: 918 1599 BsrDI GCAATG_nn' Cuts at: 0 1302 2517 Size: 1302 1215 BstXI CCAn_nnnn'nTGG Cuts at: 0 1945 2517 Size: 1945 572 BstYI r'GATC_y Cuts at: 0 263 876 978 2478 2517 Size: 263 613 102 1500 39 Fragments arranged by size: 1500 613 263 102 39 Bsu36I CC'TnA_GG Cuts at: 0 872 2517 Size: 872 1645 BtgI C'CryG_G Cuts at: 0 497 767 2517 Size: 497 270 1750 Fragments arranged by size: 1750 497 270 BtsI GCAGTG_nn' Cuts at: 0 470 1386 2517 Size: 470 916 1131 Fragments arranged by size: 1131 916 470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1284 1319 2517 Size: 1284 35 1198 Fragments arranged by size: 1284 1198 35 DraI TTT'AAA Cuts at: 0 2451 2517 Size: 2451 66 DraIII CAC_nnn'GTG Cuts at: 0 840 2517 Size: 840 1677 EaeI y'GGCC_r Cuts at: 0 365 2262 2517 Size: 365 1897 255 Fragments arranged by size: 1897 365 255 EarI CTCTTCn'nnn_ Cuts at: 0 305 350 647 1135 2517 Size: 305 45 297 488 1382 Fragments arranged by size: 1382 488 305 297 45 EcoICRI GAG'CTC Cuts at: 0 798 2517 Size: 798 1719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 167 289 304 329 479 562 607 Size: 141 26 122 15 25 150 83 45 Cuts at: 607 706 1127 1341 1652 1824 2286 2371 2454 Size: 99 421 214 311 172 462 85 83 Cuts at: 2454 2517 Size: 63 Fragments arranged by size: 462 421 311 214 172 150 141 122 99 85 83 83 63 45 26 25 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 327 2517 Size: 327 2190 EcoO109I rG'GnC_Cy Cuts at: 0 187 629 1283 1850 2517 Size: 187 442 654 567 667 Fragments arranged by size: 667 654 567 442 187 EcoRI G'AATT_C Cuts at: 0 1544 2517 Size: 1544 973 EcoRV GAT'ATC Cuts at: 0 2160 2517 Size: 2160 357 HaeII r_GCGC'y Cuts at: 0 156 523 847 2517 Size: 156 367 324 1670 Fragments arranged by size: 1670 367 324 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 321 353 814 846 1134 1166 1671 1703 Size: 321 32 461 32 288 32 505 32 Cuts at: 1703 2463 2495 2517 Size: 760 32 22 Fragments arranged by size: 760 505 461 321 288 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 933 2517 Size: 933 1584 HindIII A'AGCT_T Cuts at: 0 1668 2517 Size: 1668 849 KasI G'GCGC_C Cuts at: 0 843 2517 Size: 843 1674 MluI A'CGCG_T Cuts at: 0 1764 2517 Size: 1764 753 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2077 2517 Size: 2077 440 MscI TGG'CCA Cuts at: 0 2264 2517 Size: 2264 253 MslI CAynn'nnrTG Cuts at: 0 109 1262 1647 1731 1799 2330 2387 2517 Size: 109 1153 385 84 68 531 57 130 Fragments arranged by size: 1153 531 385 130 109 84 68 57 MspA1I CmG'CkG Cuts at: 0 46 1155 1274 2432 2517 Size: 46 1109 119 1158 85 Fragments arranged by size: 1158 1109 119 85 46 NarI GG'CG_CC Cuts at: 0 844 2517 Size: 844 1673 NcoI C'CATG_G Cuts at: 0 767 2517 Size: 767 1750 NdeI CA'TA_TG Cuts at: 0 1608 2517 Size: 1608 909 PasI CC'CwG_GG Cuts at: 0 323 2517 Size: 323 2194 PflMI CCAn_nnn'nTGG Cuts at: 0 639 2517 Size: 639 1878 PfoI T'CCnGG_A Cuts at: 0 266 539 683 2517 Size: 266 273 144 1834 Fragments arranged by size: 1834 273 266 144 PpuMI rG'GwC_Cy Cuts at: 0 187 629 1850 2517 Size: 187 442 1221 667 Fragments arranged by size: 1221 667 442 187 PstI C_TGCA'G Cuts at: 0 481 2517 Size: 481 2036 PvuII CAG'CTG Cuts at: 0 46 2432 2517 Size: 46 2386 85 Fragments arranged by size: 2386 85 46 SacI G_AGCT'C Cuts at: 0 800 2517 Size: 800 1717 SanDI GG'GwC_CC Cuts at: 0 629 2517 Size: 629 1888 SapI GCTCTTCn'nnn_ Cuts at: 0 1135 2517 Size: 1135 1382 SexAI A'CCwGG_T Cuts at: 0 1247 2517 Size: 1247 1270 SfcI C'TryA_G Cuts at: 0 477 1593 2517 Size: 477 1116 924 Fragments arranged by size: 1116 924 477 SfoI GGC'GCC Cuts at: 0 845 2517 Size: 845 1672 SmaI CCC'GGG Cuts at: 0 193 2517 Size: 193 2324 SmlI C'TyrA_G Cuts at: 0 394 1484 2493 2517 Size: 394 1090 1009 24 Fragments arranged by size: 1090 1009 394 24 SspI AAT'ATT Cuts at: 0 1435 2517 Size: 1435 1082 StyI C'CwwG_G Cuts at: 0 767 2517 Size: 767 1750 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 315 520 828 1183 1306 2517 Size: 25 290 205 308 355 123 1211 Fragments arranged by size: 1211 355 308 290 205 123 25 TatI w'GTAC_w Cuts at: 0 119 2517 Size: 119 2398 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1606 1638 2517 Size: 1606 32 879 Fragments arranged by size: 1606 879 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 789 2517 Size: 789 1728 XmaI C'CCGG_G Cuts at: 0 191 2517 Size: 191 2326 XmnI GAAnn'nnTTC Cuts at: 0 1312 1479 1669 1726 1837 2517 Size: 1312 167 190 57 111 680 Fragments arranged by size: 1312 680 190 167 111 57 Enzymes that do cut: AclI AcuI AfeI AflII AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI PasI PflMI PfoI PpuMI PstI PvuII SacI SanDI SapI SexAI SfcI SfoI SmaI SmlI SspI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DrdI EagI EciI FalI FseI FspI FspAI HpaI KpnI MfeI NaeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI ZraI