(Circular) (Six-base) MAPSORT of: pFN21AE0691.seq Check: 9123 from: 1 to: 5548 pFN21AE0691 5548 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2659 3358 278 Size: 53 83 186 936 1123 699 2468 Fragments arranged by size: 2468 1123 936 699 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4995 5263 1632 Size: 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 2616 4379 2616 Size: 1763 3785 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 2093 3291 4863 2093 Size: 1198 1572 2778 Fragments arranged by size: 2778 1572 1198 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2033 2398 2873 1870 Size: 73 82 8 365 475 4545 Fragments arranged by size: 4545 475 365 82 73 8 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2425 2869 4324 618 Size: 325 217 627 230 408 444 1455 1842 Fragments arranged by size: 1842 1455 627 444 408 325 230 217 BanII G_rGCy'C Cuts at: 1391 2505 2867 1391 Size: 1114 362 4072 Fragments arranged by size: 4072 1114 362 BbeI G_GCGC'C Cuts at: 2021 2021 Size: 5548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 928 Size: 971 359 4218 Fragments arranged by size: 4218 971 359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2059 2093 3739 3773 1903 Size: 34 122 34 1646 34 3678 Fragments arranged by size: 3678 1646 122 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2700 3440 4650 2700 Size: 740 1210 3598 Fragments arranged by size: 3598 1210 740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2156 4159 Size: 107 122 71 631 605 343 141 2003 Cuts at: 4159 136 Size: 1525 Fragments arranged by size: 2003 1525 631 605 343 141 122 107 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2430 2675 3609 4765 1790 Size: 640 245 934 1156 2573 Fragments arranged by size: 2573 1156 934 640 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2119 2119 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2300 2363 2489 2504 2813 4208 1239 Size: 986 75 63 126 15 309 1395 2579 Fragments arranged by size: 2579 1395 986 309 126 75 63 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3673 4538 4836 3673 Size: 865 298 4385 Fragments arranged by size: 4385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2548 4211 882 Size: 617 1049 1663 2219 Fragments arranged by size: 2219 1663 1049 617 BsaAI yAC'GTr Cuts at: 493 1204 2515 3339 493 Size: 711 1311 824 2702 Fragments arranged by size: 2702 1311 824 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2656 3355 3737 Size: 53 83 186 936 485 638 699 382 Cuts at: 3737 4372 275 Size: 635 1451 Fragments arranged by size: 1451 936 699 638 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2616 3981 4379 4653 4800 5211 2616 Size: 1365 398 274 147 411 2953 Fragments arranged by size: 2953 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 2273 2303 1178 Size: 30 165 30 696 30 144 30 4423 Fragments arranged by size: 4423 696 165 144 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 2744 1186 Size: 1429 129 3990 Fragments arranged by size: 3990 1429 129 BseYI C'CCAG_C Cuts at: 2082 2299 2788 4751 5247 2082 Size: 217 489 1963 496 2383 Fragments arranged by size: 2383 1963 496 489 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4975 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3759 4787 1540 Size: 275 186 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2505 2700 3609 3694 4765 2505 Size: 195 909 85 1071 3288 Fragments arranged by size: 3288 1071 909 195 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 BsmBI CGTCTCn'nnnn_ Cuts at: 2528 2528 Size: 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2505 2675 2700 2867 3609 3694 Size: 399 640 75 170 25 167 742 85 Cuts at: 3694 4765 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 640 399 170 167 85 75 25 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2813 4043 4217 65 Size: 1183 1565 1230 174 1396 Fragments arranged by size: 1565 1396 1230 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2616 4192 4379 1700 Size: 110 806 1576 187 2869 Fragments arranged by size: 2869 1576 806 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2382 2878 3245 3630 3647 5099 5185 5197 Size: 410 496 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 496 410 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 513 Size: 552 939 114 3943 Fragments arranged by size: 3943 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DraIII CAC_nnn'GTG Cuts at: 2133 2133 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2145 2238 Size: 54 1425 50 275 18 177 138 93 Cuts at: 2238 2448 2995 3884 5513 8 Size: 210 547 889 1629 43 Fragments arranged by size: 1629 1425 889 547 275 210 177 138 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 1353 Size: 2125 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2503 2503 Size: 5548 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2300 2363 2489 2504 2813 3611 Size: 393 593 75 63 126 15 309 798 Cuts at: 3611 4208 4995 5263 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 798 787 597 593 393 309 268 126 75 63 15 EcoO109I rG'GnC_Cy Cuts at: 2029 2304 2416 2749 2029 Size: 275 112 333 4828 Fragments arranged by size: 4828 333 275 112 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FspI TGC'GCA Cuts at: 2366 4054 2366 Size: 1688 3860 HaeII r_GCGC'y Cuts at: 1678 2021 4695 1678 Size: 343 2674 2531 Fragments arranged by size: 2674 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2017 2017 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2090 2258 2278 2964 3641 4789 5034 1606 Size: 484 168 20 686 677 1148 245 2120 Fragments arranged by size: 2120 1148 686 677 484 245 168 20 NarI GG'CG_CC Cuts at: 2018 2018 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4057 Fragments arranged by size: 4057 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PmlI CAC'GTG Cuts at: 2515 2515 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2029 2304 2029 Size: 275 5273 PshAI GACnn'nnGTC Cuts at: 1613 2061 2540 1613 Size: 448 479 4621 Fragments arranged by size: 4621 479 448 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2316 2334 2900 830 Size: 1486 18 566 3478 Fragments arranged by size: 3478 1486 566 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2090 2258 2278 2090 Size: 168 20 5360 Fragments arranged by size: 5360 168 20 SacI G_AGCT'C Cuts at: 2505 2505 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SanDI GG'GwC_CC Cuts at: 2029 2029 Size: 5548 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2312 2330 2896 4031 4712 4903 826 Size: 219 1267 18 566 1135 681 191 1471 Fragments arranged by size: 1471 1267 1135 681 566 219 191 18 SfoI GGC'GCC Cuts at: 2019 2019 Size: 5548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2027 2400 2875 1872 Size: 155 373 475 4545 Fragments arranged by size: 4545 475 373 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 3652 4553 4815 820 Size: 197 926 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 2159 1517 Size: 642 4906 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 2421 2660 2987 513 Size: 552 939 156 261 239 327 3074 Fragments arranged by size: 3074 939 552 327 261 239 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3575 3592 3745 3930 4375 325 Size: 1255 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3794 5506 96 Size: 274 80 33 51 1443 1817 1712 138 Fragments arranged by size: 1817 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 5508 5540 2273 Size: 32 3203 32 2281 Fragments arranged by size: 3203 2281 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2158 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2025 2398 2873 1870 Size: 155 373 475 4545 Fragments arranged by size: 4545 475 373 155 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2657 3356 276 Size: 53 83 186 936 1123 699 2468 Fragments arranged by size: 2468 1123 936 699 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI