Restriction map for
pFN21AE0680
(Circular) (Six-base) MAPSORT of: pFN21AE0680.seq Check: 5066 from: 1 to: 5902
pFN21AE0680 5902 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 3965 5349 5617 1632
Size: 1193 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1193 1140 268
AfeI AGC'GCT
Cuts at: 2639 2639
Size: 5902
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2553 4801 5778 1203
Size: 1350 2248 977 1327
Fragments arranged by size:
2248 1350 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2964 2996 2964
Size: 32 5870
AlwNI CAG_nnn'CTG
Cuts at: 2089 2110 3645 5217 2089
Size: 21 1535 1572 2774
Fragments arranged by size:
2774 1572 1535 21
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3211 3437 3589 5698 1493
Size: 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2901 3227 1870
Size: 73 339 619 326 4545
Fragments arranged by size:
4545 619 339 326 73
BamHI G'GATC_C
Cuts at: 2241 3232 3599 2241
Size: 991 367 4544
Fragments arranged by size:
4544 991 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2730 2901 3221 1391
Size: 733 606 171 320 4072
Fragments arranged by size:
4072 733 606 320 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2831 928
Size: 971 932 3999
Fragments arranged by size:
3999 971 932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 2829 1318
Size: 1511 4391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2174 2654 2939 4562 1239
Size: 842 93 480 285 1623 2579
Fragments arranged by size:
2579 1623 842 480 285 93
Bpu10I CC'TnA_GC
Cuts at: 2696 2696
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2556 3693 493
Size: 711 1352 1137 2702
Fragments arranged by size:
2702 1352 1137 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 3709 4091 4726
Size: 53 83 186 936 483 1693 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1693 1451 936 635 483 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2041 2190 2487 3063 4335 4733 5007 5154 5565
Size: 149 297 576 1272 398 274 147 411
Cuts at: 5565 2041
Size: 2378
Fragments arranged by size:
2378 1272 576 411 398 297 274 149
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2132 2300 2702 3101 1186
Size: 874 72 168 402 399 3987
Fragments arranged by size:
3987 874 402 399 168 72
BseYI C'CCAG_C
Cuts at: 2508 2809 5105 5601 2508
Size: 301 2296 496 2809
Fragments arranged by size:
2809 2296 496 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2447 1163
Size: 573 711 4618
Fragments arranged by size:
4618 711 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3352 4113 5141 1540
Size: 275 186 1033 318 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 318 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2382 3963 4048 5119 2124
Size: 258 1581 85 1071 2907
Fragments arranged by size:
2907 1581 1071 258 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2382 2730 2901 3221 3963 4048
Size: 399 334 258 348 171 320 742 85
Cuts at: 4048 5119 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 334 320 258
171 85
BspEI T'CCGG_A
Cuts at: 2041 5565 2041
Size: 3524 2378
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3257 844
Size: 1844 569 3489
Fragments arranged by size:
3489 1844 569
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 3789 1394
Size: 429 227 1739 3507
Fragments arranged by size:
3507 1739 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 2208 4397 4571 65
Size: 1183 960 2189 174 1396
Fragments arranged by size:
2189 1396 1183 960 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 2304 96
Size: 2208 3694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4607
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2241 2395 3066 3232 3599 3984 4001 5453
Size: 269 154 671 166 367 385 17 1452
Cuts at: 5453 5539 5551 5897 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 671 385 367 346 269 166
154 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 2685 2742 2435
Size: 250 57 5595
Fragments arranged by size:
5595 250 57
BtgI C'CryG_G
Cuts at: 513 1065 2004 2886 513
Size: 552 939 882 3529
Fragments arranged by size:
3529 939 882 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 3418 4212 4232 2382
Size: 1036 794 20 4052
Fragments arranged by size:
4052 1036 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 3058 3058
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3031 3349
Size: 54 1425 50 275 18 816 385 318
Cuts at: 3349 4238 5867 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 816 385 318 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 3349 1537
Size: 275 1219 318 4090
Fragments arranged by size:
4090 1219 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3832 1353
Size: 2479 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 3041 4489 4863 5009 387
Size: 1064 138 503 949 1448 374 146 1280
Fragments arranged by size:
1448 1280 1064 949 503 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2174 2654 2825 2939 3965 4562
Size: 393 449 93 480 171 114 1026 597
Cuts at: 4562 5349 5617 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1026 787 597 480 449 393 268
171 114 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2306 2684 2114
Size: 192 378 5332
Fragments arranged by size:
5332 378 192
EcoO109I rG'GnC_Cy
Cuts at: 2431 2677 2431
Size: 246 5656
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2110 2641 2842 5049 1678
Size: 432 531 201 2207 2531
Fragments arranged by size:
2531 2207 531 432 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2895 2927 4543 4575 4617
Size: 32 135 32 1323 32 1616 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 1616 1323 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 10
Size: 54 1425 343 816 3264
Fragments arranged by size:
3264 1425 816 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3318 3995 5143 5388 1606
Size: 1712 677 1148 245 2120
Fragments arranged by size:
2120 1712 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4411
Fragments arranged by size:
4411 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2531 1348
Size: 1183 4719
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 4759
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PmlI CAC'GTG
Cuts at: 2556 2556
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2677 2677
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2274 3254 830
Size: 1444 980 3478
Fragments arranged by size:
3478 1444 980
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
RsrII CG'GwC_CG
Cuts at: 2785 2785
Size: 5902
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2270 3250 4385 5066 5257 826
Size: 219 1225 980 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 980 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 1517
Size: 984 4918
StyI C'CwwG_G
Cuts at: 513 1065 2004 3341 513
Size: 552 939 1337 3074
Fragments arranged by size:
3074 1337 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 3946 4099 4284 4729 325
Size: 1255 2349 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2880 4148
Size: 274 80 33 51 1443 327 576 1268
Cuts at: 4148 5860 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1268 576 327 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 2015 1316
Size: 699 5203
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2140 2140
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2216 4031 2216
Size: 1815 4087
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI PvuII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI